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In general, enzyme activity increases with increasing temperature until it reaches maximum activity. The temperature at which the enzyme is the most active is the optimal temperature. Increasing the temperature beyond the optimum denatures the enzyme, making it inactive.
In general, enzyme activity increases with increasing temperature until it reaches maximum activity. The temperature at which the enzyme is the most active is the optimal temperature. Increasing the temperature beyond the optimum denatures the enzyme, making it inactive.
Use data provided for each enzyme shown below to prepare a graph of product vs. temperature. Use the graph to determine the temperature at which the enzyme is the most active.
Use data provided for each enzyme shown below to prepare a graph of product vs. temperature. Use the graph to determine the temperature at which the enzyme is the most active.
Reaction Conditions
Reaction Conditions
The following amounts of temperatures were tested for each set of experiments
The following amounts of temperatures were tested for each set of experiments
0 °C
10 °C
20 °C
30 °C
40 °C
50 °C
60 °C
70 °C
80 °C
90 °C
100 °C
Shown below are the Incubation Conditions that were used
Shown below are the Incubation Conditions that were used
pH Optimal pH for each enzyme
Amount of Enzyme 100 ul (unless otherwise noted)
Incubation Time 10 minutes (unless otherwise noted)
Substrate Concentration 100 mM
Results - Temperature
Results - Temperature
Select an enzyme to see the results of varying the Temperature on enzyme activity. These results are also shown in graphical form at the bottom of the page.
Select an enzyme to see the results of varying the Temperature on enzyme activity. These results are also shown in graphical form at the bottom of the page.
Enzyme 1
Enzyme 1
Temp (˚C) product (µmol)
0 5.99
10 11.18
20 19.18
30 32.21
40 48.74
50 66.01
60 65.57
70 0.04
80 0.03
90 0.05
100 0
Enzyme 2
Enzyme 2
Temp (˚C) product (µmol)
0 6.18
10 11.57
20 20.15
30 32.23
40 47.47
50 55.89
60 30.65
70 0.04
80 0.03
90 0.02
100 0.01
Enzyme 3
Enzyme 3
Temp (˚C) product (µmol)
0 2.45
10 4.44
20 7.79
30 12.84
40 19.75
50 26.15
60 16.15
70 0.03
80 0.04
90 0.01
100 0.03
Enzyme 4
Enzyme 4
Temp (˚C) product (µmol)
0 5.37
10 9.71
20 17.04
30 28.12
40 43.88
50 61.51
60 76.2
70 71.3
80 0.02
90 0.02
100 0.04
Enzyme 5
Enzyme 5
Temp (˚C) product (µmol)
0 21.35
10 39.34
20 70.46
30 113.57
40 169.93
50 214.3
60 221.56
70 152.35
80 0.01
90 0.03
100 0.03
Graphs showing the results of varying pH on enzyme activity are shown below for each enzyme.
Graphs showing the results of varying pH on enzyme activity are shown below for each enzyme.
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