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Enzymes will lose activity over time. The data provided for each enzyme were used to plot graphs of product vs time, which can be found at the bottom of this page.
Enzymes will lose activity over time. The data provided for each enzyme were used to plot graphs of product vs time, which can be found at the bottom of this page.
Find the linear range of the graph, this indicates that the enzyme has been active at more or less constant rate.
Find the linear range of the graph, this indicates that the enzyme has been active at more or less constant rate.
Use a time that falls within this linear region for all subsequent reactions.
Use a time that falls within this linear region for all subsequent reactions.
Reaction Conditions
Reaction Conditions
The following incubation times were tested for each enzyme:
The following incubation times were tested for each enzyme:
0.0 min
6.0 min
12.0 min
18.0 min
24.0 min
30.0 min
36.0 min
42.0 min
48.0 min
54.0 min
60.0 min
Shown below are the incubation conditions that were used:
Shown below are the incubation conditions that were used:
pH Optimal pH for each enzyme
Amount of Enzyme 100 ul (Unless otherwise noted)
Temperature 30 degrees Celsius
Substrate Concentration 100 mM
Results - Incubation Time
Results - Incubation Time
Select an enzyme to see the results of varying incubation time on enzyme activity. These results are also shown in graphical form at the bottom of the page.
Select an enzyme to see the results of varying incubation time on enzyme activity. These results are also shown in graphical form at the bottom of the page.
Enzyme 1
Enzyme 1
min product (µmol)
0 0.26
6 14.97
12 29.31
18 40.65
24 51.49
30 61.9
36 69.65
42 76.84
48 82.45
54 86.78
60 90.17
Enzyme 2
Enzyme 2
min product (µmol)
0 0.27
6 15.7
12 29.95
18 42.62
24 53.98
30 64.09
36 73.89
42 78.69
48 84.98
54 88.63
60 92.7
Enzyme 3
Enzyme 3
min product (µmol)
0 0.23
6 13.51
12 25.26
18 36.48
24 45.9
30 55.18
36 61.73
42 69.89
48 74.33
54 78.38
60 82.75
Enzyme 4
Enzyme 4
min product (µmol)
0 0.25
6 14.54
12 28.25
18 43.35
24 51.57
30 62.35
36 71.86
42 79.24
48 87.22
54 89.66
60 93.8
Enzyme 5
Enzyme 5
min product (µmol)
0 0.26
6 15.34
12 30.17
18 43.67
24 53.78
30 65.32
36 74.34
42 80.58
48 87.65
54 90.05
60 95.65
Graphs showing the results of varying incubation time on enzyme activity are shown below for each enzyme.
Graphs showing the results of varying incubation time on enzyme activity are shown below for each enzyme.
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