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Enzyme-catalyzed reactions often display a hyperbolic relationship between the reaction rate and substrate.
Enzyme-catalyzed reactions often display a hyperbolic relationship between the reaction rate and substrate.
At low substrate concentration there is a steep increase in the rate of reaction with increasing substrate concentration. At higher concentrations, the enzyme becomes saturated with substrate. Adding more substrate at this point will not increase the rate of reaction. The reaction rate when the enzyme is saturated represents Vmax.
At low substrate concentration there is a steep increase in the rate of reaction with increasing substrate concentration. At higher concentrations, the enzyme becomes saturated with substrate. Adding more substrate at this point will not increase the rate of reaction. The reaction rate when the enzyme is saturated represents Vmax.
The data provided for each enzyme shown below were used to generate graphs showing substrate concentration vs. reaction rate. The optimal substrate amount will fall within the region showing maximum activity (Vmax).
The data provided for each enzyme shown below were used to generate graphs showing substrate concentration vs. reaction rate. The optimal substrate amount will fall within the region showing maximum activity (Vmax).
Reaction Conditions
Reaction Conditions
The following amounts of temperatures were tested for each set of experiments
The following amounts of temperatures were tested for each set of experiments
0 mM
25 mM
50 mM
75 mM
100 mM
125 mM
150 mM
175 mM
200 mM
225 mM
250 mM
Shown below are the Incubation Conditions that were used
Shown below are the Incubation Conditions that were used
pH Optimal pH for each enzyme
Amount of Enzyme 100 ul (unless otherwise noted)
Incubation Time 10 minutes (unless otherwise noted)
Temperature Optimal Temperature for each enzyme
Results - Substrate Amount
Results - Substrate Amount
Select an enzyme to see the results of varying the Substrate Amount on enzyme activity. These results are also shown in graphical form at the bottom of the page.
Select an enzyme to see the results of varying the Substrate Amount on enzyme activity. These results are also shown in graphical form at the bottom of the page.
Enzyme 1
Enzyme 1
substrate (mM) product (µmol)
0 0.01
25 22.62
50 40.25
75 50
100 56.49
125 59.2
150 62.16
175 63.84
200 64.66
225 64.92
250 66.49
Enzyme 2
Enzyme 2
substrate (mM) product (µmol)
0 0.01
25 23.73
50 40.37
75 51.2
100 55.93
125 59.58
150 59.82
175 61.49
200 62.4
225 63.56
250 64.99
Enzyme 3
Enzyme 3
substrate (mM) product (µmol)
0 0.01
25 13.42
50 20.32
75 23.92
100 26.81
125 27.32
150 29.02
175 30.17
200 30.09
225 31
250 31.55
Enzyme 4
Enzyme 4
substrate (mM) product (µmol)
0 0.04
25 22.88
50 41.53
75 53.73
100 61.76
125 66.2
150 70.14
175 71.55
200 73.32
225 75.1
250 76.27
Enzyme 5
Enzyme 5
substrate (mM) product (µmol)
0 0.04
25 24.73
50 46.9
75 68.12
100 82.02
125 93.08
150 102.43
175 107
200 109.67
225 113.63
250 113.51
Graphs showing the results of varying pH on enzyme activity are shown below for each enzyme.
Graphs showing the results of varying pH on enzyme activity are shown below for each enzyme.
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