Week 6
Major concept goals
-Most proteins added to Golgi are not residents, but some are!
-Proteins leave the Golgi from the TGN (trans Golgi network) and can move to the cell surface (secretion) or endosomes via vesicles
-Proteins added to the lysosome come from the cell surface and the TGN
-How vesicle transport, organelle maturation, and organelle fusion mediate the delivery of proteins to lysosomes.
Overview of content
I. Discussion: Does race matter in cell biology?
II. Most proteins in the Golgi are not residents, they are on their way to another place
A. TGN to PM
B. TGN to endosomes
III. How are lysosomes made?
A. Mechanism of endocytosis
1. Recycling to the cell surface vs
2. transport to the lysosome
B. Mechanism of transport from the TGN to the early endosome
1. Creation of the M-6-P signal in the Golgi
2. The receptors for M-6-P in the TGN
3. How the receptors deliver cargo to the EE and how they are recycled back to the TGN
C. Moving proteins from early endosomes to late endosomes
1. Organelle maturation
2. Rabs in organelle maturation (Rab5 and Rab7)
3. How changing Rabs leads to differences between early and late endosomes
D. Moving cargo into lysosomes via fusion between late endosomes and lysosomes
IV. Considering how proteins are added to different organelles: mitochondria, ER, Golgi, early endosomes, late endosomes, lysosomes