Week 10
Major concept goals
-The stages of the nuclear cycle
-MPF is sufficient for promoting M-phase
-MPF is cyclin bound to a cyclin dependent kinase
-How cyclin level oscillate and the role of protein degradation
-There is a negative feedback loop during M-phase so that active MPF leads to the degradation of cyclin and inactivation of MPF via APC
-How Wee1 and Cdc25 regulate MPF activity during interphase
Overview of content
I. Cell division
A. What happens during cell division
B. What needs to happen for a successful cell division
1. Duplication
2. Segregation
C. The nuclear cycle (often called the cell cycle)
1. DNA content during the nuclear cycle
II. Regulation of the nuclear cycle
A. What needs to be regulated?
B. Identification of MPF using cell-cell fusion experiments
C. How to identify MPF?
D. MFP is a Ser/Thr protein kinase that is present throught the nuclear cycle
E. How is MPF activity regulated so that it is only "on" (kinase activity on) during M-phase?
F. Identification and function of cyclin in regulating MPF activity.
G. Cyclin levels controlled by APC
H. Model for inactivating MPF after it becomes activated in M-phase
I. How MPF activity is regulated prior to M-phase by Wee1 and Cdc25