Week 5
Major concept goals
-Golgi structure and function
-How a pulse-chase analysis of protein secretion informs how proteins might be added to the Golgi
-How yeast mutants identified the machinery that mediates protein delivery to the Golgi and how double mutants can be used to determine the order of steps in the pathway
-How proteins are targeting to and transported into the ER
-How vesicles are made by coat proteins on a donor organelle
-How Arfs recruit coat proteins to a membrane
-The GTPase cycle in regulating protein-protein interactions
-How Rabs target vesicles by interacting with tethers in the target organelle
-How SNAREs catalyze membrane fusion
Overview of content
I. Making the Golgi
A. Golgi structure and function
B. Using a pulse-chase experiment to analyze how proteins are added to the Golgi
C. Using yeast mutants to analyze how proteins are added to the Golgi
D. The model is that proteins are not added directly to the Golgi, but are first added to the ER and then transported to the Golgi
II. Adding proteins to the ER
A. Structure and function of the ER
B. Signal sequence for targeting and transport of proteins to the ER
C. SRP and protein import pore
III. Adding proteins to the Golgi (moving proteins from the ER to the Golgi via vesicles)
A. Making vesicles and the role of Arfs and coats
B. Docking vesicles and the role of Rabs and tethers
C. Fusing vesicles to their target and the role of SNAREs