Practical Exercises

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Exercise 1

Understand Reverse/forwards strands and reverse complementary strand

Exercise 2

Translate DNA sequence into protein

Exercise 3

Access to Databases

Exercise 4

Access to protein databank

Exercise 5

Drawing DNA Molecule

Exercise 6

Restriction Digestion (combined to restriction enzyme theory lecture)

Exercise 7

Search Gene sequence and understand file formats in databases

Convert Non-FASTA into FASTA format

(follow up of exercise 3 )

Exercise 8

Using BLAST to identify a gene

(follow up of exercise 7)

Exercise 9

Multiple Sequence Alignment (MSA)

(follow up of exercise 8)

Exercise 10

Sequence Alignment and Phylogenetic Analysis using various software

• ClustalX
• Bioedit
• Figtree
• Phylip

Exercise 11

Sequence Alignment and Phylogenetic Analysis using various software/ Web servers

Exercise 12

HIV sequence alignment exercise

Exercise 13

Swiss-prot Structure superpose