Exercise 8

Using BLAST to identify a gene

(follow up of exercise 7)

Using BLAST to identify a gene

Select the BLAST program that is required protein / DNA
Copy and paste your longest translated protein sequence /DNA into the search box.
Select the required Database
Select the standard Protein-Protein BLAST (BLASTP) while searching protein or Megablast for DNA
For the first time, leave the Options section settings on the Default values
Click on the Blast button at the bottom or top of the screen.
A new window will appear gives an estimate of how long the search will take and which lists conserved domains in your query sequence.
You may want to copy your request id number, but usually this isn't necessary. After the indicated time has passed, press the Format button to see your results
Check your result - check graphic - red, green, blue, black lines and their meaning
Identify the sequence and analyse
Check E value, coverage, identity with other sequences
Check the ID of the result sequences
Open related literature
Save the results
You can download multiple sequences in a single file
Save file, give it a name, you will use it for multiple sequence alignment

Sequences for analysis by BLAST

>Example

CGAGGGACCTTTACGGCGTAATCCTGGAAACCATGACAAATCCAGAACCCCAAGGCTCCCCTCTT

CAGCTGATGTAGAATTTTGCCTGAGTTTGACCCATGGAAGGATTTGCTAGTCCACTTACTGGGAT

AGCGGATGCCTCTCAAAGAGCATGCACAATGCCTTGCACATCTATATGAATGGAACAATGTCCCA

GGCAGGGATCTGCCAACGATCCTATCTTCCTTCTTCACCATGCATTTGTTGACAGTATTTTTGAG

CAGTGGCTCCGAAGGCACCGTCCTCTTCAAGAAGTTTATCCAGAAGCCAATGCACCCATTGGACA

TAACCGGGAATCCTACATGGTTCTTATACCACTGTACAGAAATGGTGATTTCTTTATTTCATCCA

AAGATCTGGGCTATGACTATAGCTATCTACAAGATTCAGACCCAGACTCTTTTCAAGACTACATT

AAGTCCTATTTGGAACAAGCGAGTCGGATCTGGTCATGGCTCCTTGGGGCGGCGATGGTAGGGGC

CGTCCTCACTGCCCTGCTGGCGGGCTTGTGAGCTTGCTGTGTCGTCACAAGAGAAAGCAGCTTCC

TGAAGAAAAGCAGCCACTCCTCATGGAGAAAGAGGATTACCACAGCTTGTATCAGAGCCATTTAT

AAAAGGCTTAGGCAATAGAGTAGGGCCAAAAAGCCTGACCTCACTCTAACTCAAAGTAATGTCCA

GGTTCCCAGAGAATATCTGCTGGTATTTTTCTGTAAAGACCATTTGCAAAATTGTAACCTAATAC

AAAGTGTAGCCTTCTTCCAACTCAGGTAGAACACACCTGTCTTTGTCTTGCTGTTTTCACTCAGC

CCTTTTAACATTTTCCCCTAAGCCCATATGTCTAAGGAAAGGATGCTATTTGGTAATGAGGAACT

GTTATTTGTATGTGAATTAAAGTGCTCTTATTTTAAAAAATTGAAATAATTTTGATTTTTGCCTT

CTGATTATTTAAAGATCTATATATGTTTTATTGGCCCCTTCTTTATTTTAATAAAACAGTGAGAA

ATCT

>Sequence 1

TCGAAATAACGCGTGTTCTCAACGCGGTCGCGCAGATGCCTTTGCTCATCAGATGCGACCGCAAC

CACGTCCGCCGCCTTGTTCGCCGTCCCCGTGCCTCAACCACCACCACGGTGTCGTCTTCCCCGAA

CGCGTCCCGGTCAGCCAGCCTCCACGCGCCGCGCGCGCGGAGTGCCCATTCGGGCCGCAGCTGCG

ACGGTGCCGCTCAGATTCTGTGTGGCAGGCGCGTGTTGGAGTCTAAA

>Sequence 2

GTTTATTAGTGATCATGGCTAAGTTTGCGTCCATCATCGCACTTCTTTTTGCTGCTCTTGTTCTTT

TTGCTGCTTTCGAAGCACCAACAATGGTGGAAGCACAGAAGTTGTGCGAAAGGCCAAGTGGGACAT

GGTCAGGAGTCTGTGGAAACAATAACGCATGCAAGAATCAGTGCATTAACCTTGAGAAAGCACGAC

ATGGATCTTGCAACTATGTCTTCCCAGCTCACAAGTGTATCTGCTACTTTCCTTGTTAATTTATCG

CAAACTCTTTGGTGAATAGTTTTTATGTAATTTACACAAAATAAGTCAGTGTCACTATCCATGAGT

GATTTTAAGACATGTACCAGATATGTTATGTTGGTTCGGTTATACAAATAAAGTTTTATTCACCA

>Sequence 3

CTCGAGACTAGTTCTCTCTCTCTCTCTCTCGTGCCGCATCTCACACCTGTGGATGGACGGCAGCTG

AACCGCGGGAAACTTTCGTTCTCACTCTACCTAGATGAACTTTAGTTTATATTAAACACGCGTCGA

CTCCCACACAAACCGTGCTCGTTTTACATCTTTGTCTCCGCTTTTGAAAACGAGAAGTTGAATTCG

CAAGACGCAACTTTCCAGCCCCTCACTGAGCGGGCAGAGTCCGTGAAGCGATGGAGCCGTCCGTCA

TTCCCGGTGCTGACATACCCGACCTTTACTCCATTAACCCGTTTAATGTCACTTTTCCCGACGACG

TTTTGAGTTTCGTTCCTGATGGGAGGAACTACACCGAACCTAACCCGGTAAAGAGCCGCGGAATCA

TCATCGCCATTTCCATCACCGCTC

>Sequence 4

GACATTACGGCGACCCAGTCTCCCCCGGTGTTGTCAGTGGGACTGGGCCAGACCGCAACCATCACTT

GTACGGCCAGTCAAAGCATCTACAGTAACCTTGCTTGGTACCAGCAGAGAGAAGGACAGAAGCCCTC

TCTCCTGATCTATGCTGCGACAACGCGATACGAAGGAGTCTCCGAGCGATTCAGCGGCAGTGGATCA

GGGACCAGTTTCACCCTGACAATCAGCAACGTTCAGAATGAGGATGTCGCTGACTATTACTGTCAGA

TCGCATATTCGATCTACTCCGGTTCCGTTGTTTTCGGTGAAGGAACCAAGCTCAGACTGAGCCGT

>Sequence 5

GAATTCGCGGCCGCATGGGGGAGAAGCTGCCGGTTGTGTATAAACGCTTCATCTGCTCGTTCCCGGA

TTGTAATGCCACGTATAACAAGAACCGGAAGCTGCAGGCCCATCTGTGCAAGCACACGGGGGAGAGA

CCGTTTCCTTGCACATATGAAGGCTGTGAGAAAGGCTTTGTGACGCTGCATCACCTGAATCGTCATG

TGCTCTCCCACACCGGGGAGAAACCCTGCAAATGCGAAACGGAAAATTGCAATTTGGCGTTCACCAC

AGCATCCAACATGAGGTTGCACTTCAAAAGGGCTCATTCTTCTCCGGCGCAGGTCTACGTGTGTTAT

TTCGCAGACTGTGGCCAGCAGTTCAGGAAACATAACCAGCTAAAAATTCACCAGTATATCCATACAA

ACCAGCAACCCTTCAAAT

>Sequence 6

GCCCAGCGTCTCTCGGAGGAAGCTAATTCTCAGGTTATCGCAGAGGAATCTCTTGTAGCTCGTGCTGA

GGCTACCGTTGTCCAAGCCGCCGCTCCAACCAAATCCCTTGATCTGACAACATGGAAGTATGCTGATC

TCAGAGACACTATCAACACCTCAATCGATATTGCGCTCCTGTCAGCCTGCAAGGAGGAGTTCCATCGT

CGTCTCAAGGTCTACCACGCCTGGAAGATGAAGAATAAGAAGGTTGCCGCCGGCGACAAGGGCGGACC

AGAGAGGGCTCCACAATCCATCTTTGAAAGTGCCCAACAATACAACCAGCTGGCACCCCCTCCGAAAG

CCACCAAGGCTGCCCCAGCCAATCAGAACATCCAACGCTTCTTCAGGGTGCCTTTCTCCGTGACTGGG

TCCACCGCTCAGGGTCAGATGCCCGAGAGGGGTTGGTGGTACGCCCACTTTGACGGTCAGTGGATCGC

CCGCCAGATGGAGGTACACCCCACCAAGGTCCCCGTTCTTCTGGTTGCAGGTAAAGATGATGAGAACA

TGTGTGAGATGAGTTTGGAGGAGACTGGGTTGACACGACGTCCCAACGCCGAGATCGTCGAGCGGGAG

TTTGAGGAGCCCTGGAAGCGTAGCGGCGGTCAGCAGTACCACATGGCTGCAGTACGCAACAAGCAGGC

TAGACCAACGTGGGCCACGCAGAGCTTGAA

>Sequence 7

AACAATTCATTTTTCCTGCTTTCCTAGAAAATTCTATAAAAGCTTCAAAATGAATTACTTGGTGATGA

TTAGTTTGGCACTTCTCTTCGTGACAGGTGTAGAGAGTGTAAAAGACGGTTATATTGTCGACGATGTA

AACTGCACATACTTTTGTGGTAGAAATGCATACTGCAACGAGGAATGTACCAAGTTGAAAGGTGAGAG

TGGTTATTGCCAATGGGCAAGTCCATATGGAAACGCCTGTTATTGCTATAAATTGCCCGATCATGTAC

GTACTAAAGGACCAGGAAGATGCCATGGCCGATAAATTATAAGATGGAATGTATCCTAAGTATCAATG

TTAAATAAATATAATCAAAAAATT

>Sequence 8

ACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAAC

TGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGA

GGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTA

TTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAG

GGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCG

CGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGA

AGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCC

>Sequence 9

AACCAACCCAACTCGCCGCCGCATCGCCCTCGTTAGAACGATGGCCGCGTCGGCGCTGCACCAGACCAC

CAGCTTCCTCGGCACCGCCCCTCGCCGGGATGAGCTCGTCCGCCGCGTCGGCGACTCCGGTGGCCGCAT

CACCATGCGCCGCACCGTCAAGAGCGCGCCCCAGAGCATCTGGTATGGACCTGACCGTCCCAAGTACCT

GGGCCCGTTCTCGGAGCAGACGCCATCGTACCTGACCGGAGAGTTCCCGGGAGACTACGGGTGGGACAC

GGCGGGGCTATCGGCCGACCCGGAGACGTTCGCGAGGAACAGGGAGCTGGAGGTGATCCACTCGCGGTG

GGCGATGCTGGGGGCGCTGGGCTGCGTCTTCCCGGAGATCCTGTCCAAGAACGGGGTGAAGTTCGGGGA

GGCGGTGTGGTTCAAGGCCGGCGCGCAGATCTTCTCCGAGGGGGGGCTCGACTACCTGGGGAACCCCAA

CCTGGTGCACGCGCAGAGCATCCTCGCCATCTGGGCGGTCCAGGTGGTGCTCATGGGATTCGTCGAGGG

CTACCGCGTCGGCGGCGGCCCGCTCGGCGAGGGCCTCGACAAGGTGTACCCAAGCGGCGCCTTCGACCC

GCTCGGCCTCGCCGACGACCCTGACACCTTCGCCGAGCTCAAGGTGAAGGAGCTCAAGAACGGCCGCCT

CGCCATGTTCTCCATGTTCGGCTTCTTCGTCCAGGCCATCGTCACCGGCAAGGGCCCCATCGAGAACCT

CTTCGACCACGTCGCCGACCCCGTCGCCAACAACGCCTGGGCATACGCCACCAACTTCGTCCCCGGCAA

GTGAGCACAACGACACGATCGAGATGGTGCCAACCAACGTACCATGTGTACACTTGTAGTAGCCACGCA

CCGACCCTGCAGTTGCAGTTGCAGCAGTGCATGTATGTATGTACCTTAATTGTGTGTGTGTGTGTGATC

GATCGAGGAGATTTCTAGCTTAATTAATTTCTGTC

>Sequence 10

ACTCAACGTAAATCAGAAGATTCCAACTGCAGTGGAAGGCAGCACATGAAATAAATTACTTGTTAGAAAG

AATACTGCCAACAGCATAGCAAAATGAAATTCTTCCTGCTGCTTTCCCTCATTGGATTCTGCTGGGCCCA

ATATGACCCACATACTCAATATGGACGAACTGCTATTATCCACCTGTTTGAGTGGCGCTGGGTTGATATT

GCTAAGGAATGTGAGAGATACTTAGCTCCTAATGGATTTGCAGGTGTGCAGGTCTCTCCACCCAATGAAA

ACATCGTAGTCCACAGCCCTTCAAGACCATGGTGGGAAAGATATCAACCAATTAGCTACAAAATATGTTC

CAGGTCTGGAAATGAAGATGAATTCAGGGACATGGTGAACAGGTGCAACAATGTTGGTGTCCGTATTTAT

GTGGATGCTGTCATTAACCACATGTGTGGAGTGGGGGCTCAAGCTGGACAAAGCAGTACATGTGGAAGTT

ATTTCAACCCAAATAACAGGGACTTTCCTGGAGTTCCCTATTCTGGTTTTGACTTTAATGATGGAAAATG

TAGAACTGCAAGTGGAGGTATCGAGAACTACCAAGATGCTGCTCAGGTCAGAGATTGTCGTCTGTCTGGC

CTTCTGGATCTTGCACTTGAGAAAGATTATGTTCGAACCAAGGTGGCTGACTATATGAACCATCTCATTG

ACATTGGCGTAGCAGGGTTCAGACTTGATGCTTCTAAGCACATGTGGCCTGGAGACATAAAGGCAATTTT

GGACAAACTGCATAATCTCAATACAAAATGGTTCTCCCAAGGAAGCAGACCTTTCATTTTCCAAGAGGTG

ATTGATCTGGGTGGTGAGGCAGTGTCAAGTAATGAGTATTTTGGAAATGGCCGTGTGACAGAATTCAAAT

ATGGAGCAAAATTGGGCAAAGTTATGCGCAAGTGGGATGGAGAAAAGATGTCCTACTTAAAGAACTGGGG

AGAAGGTTGGGGTTTGATGCCTTCTGACAGAGCCCTTGTGTTTGTGGACAACCATGACAATCAGCGAGGA

CATGGTGCTGGGGGAGCATCCATCTTGACATTCTGGGATGCTAGACTCTATAAAATGGCTGTTGGCTTTA

TGTTGGCTCATCCTTATGGTTTCACACGGGTGATGTCAAGTTACTATTGGCCAAGAAATTTCCAGAATGG

AAAAGATGTCAATGACTGGGTTGGACCACCAAATAACAATGGAAAAACCAAAGAAGTGAGCATTAACCCA

GACAGCACTTGTGGCAATGACTGGATCTGTGAACATCGATGGCGTCAAATAAGGAACATGGTTGCCTTCA

GAAATGTCGTCAATGGTCAGCCTTTTGCAAACTGGTGGGATAATGACAGCAACCAGGTAGCTTTTGGCAG

AGGAAACAAAGGACTCATTGTCTTTAACAATGATGACTGGGCTTTGTCAGAAACTTTACAGACTGGTCTT

CCTGCTGGCACATACTGTGATGTCATTTCTGGAGATAAAGTCGATGGCAATTGCACTGGAATAAAAGTCT

ATGTTGGCAATGATGGCAAAGCTCACTTTTCTATTAGTAACTCTGCCGAAGACCCATTTATTGCAATCCA

TGCAGAGTCAAAAATATAAAATTTAAAATAAACACATATTGAGAGCATCA

>Sequence 11

TGCATCACAAGGTTAATGTGAAAACACAGCGAGAAGTCCATTTCCCAATGGACCTCTTGCAAGCCTGTGGT

GCATCTGCCCCTAGGCCAGTTGCCCGTGTTTCACGTGCAACCGACCTAGACCGACGCTACAGGTGCGTCCT

CAGTTTACCTGAGGAGCGTGCTCGCAGTGTTGGGTGTAAATGGTCGTCGACCCGAGCGGCGTTACGACGTG

GACTCGAGGAGCTTGGCTCCCGCGAGTTCCGCCGTCGTCTCCGTTTGGCGGACGATTGCTGGCGCGCGATC

TGCGCGGCCGTCTGCACGGGTCGGAAGTTTCCTTCCTTCTCGGTGACAGATCGGCCGGCAAGAGCTCGCCT

TGCAAAAGTCTACCGTATGGGTCGTCGACTGCTAGTAGGTGTGGTCTGCCGAGGCGAATCGGTCG

>Sequence 12

ATAACCACACGCCTTTGGCGTGATTATCAGCTTTCAAGTTTCAGTTACTAAAACTAATACTGACTATAAAA

CAGAAGCAAAAAAATTTTCGATTTTTATGAAAACGGTCGCAAAGAAGTTAGCAAAAATATATAATTTCTTT

TGAAATTGTTCACTTGGCCAAGCTGCAGTTTCAATATTTTAATAAAGGGGGCAGTAAAAAGTGAAAAAAAA

GAAAAGTTTCTGGCTTGTTTCTTTTTTAGTTATAGTAGCTAGTGTTTTCTTTATATCTTTTGGATTTAGCA

ATCATTCTAAACAAGTTGCTCAAGCGGCTAGTGATACGACATCAACTGATCACTCAAGCAATGATACAGCT

GATTCTGTTAGCGACGGTGTTATTTTGCATGCATGGTGCTGGTCGTTCAACACGATTAAAAACAACTTGAA

ACAGATTCATGACGCCGGCTACACAGCGGTTCAAACTTCACCTGTTAATGAAGTTAAAGTTGGAAATAGCG

GGTCTAAGTCATTAAATAACTGGTATTGGCTATATCAGCCAAC

>Sequence 13

GTGCGCGTTAGACCATATAAAGAAAAACCAATACAAACTCCAGCAAAATCTGTTGATATAAGATATACTGTA

CAGTTTACTCCTTTAAACCCTGATGATGATTTCAAGCCAGTTCTCAAAGATACTAAACTATTGAAAACATTA

GCTATCGGCGACACCATCACATCCCAAGAATTACTAGCTCAAGCACAAAGCATTTTAATCGAAAGCCATCCA

GATTATACGATTTATGAACGTGATTCCTCAATCGTCACTCATGACAATGACATTTTCCGTACGATTTTACCA

ACGGATCAAGAGTTTACTTACCATGTCAAAAATCGGGAACAAGCTTATAAGGCCAATTCTAAAACAGATATT

AAAGAAAAAACGAACAACACCGAC

>Sequence 14

CTAATAATCCTTGGAATACTCCTATATTTTGTATAAAGAAGAAATCAGGGAAATGGAGAATGCTAATTGATT

TTAGAGAACTTAATGCAAAAACAGAAAAAGGAGCAGAAGTCCAATTAGGATTACCTCACCCATCTGGATTAC

AGAAGAGAAAGAATGTAACAGTTTTAGATATAGGAGATGCTTATTTTACCATCCCTTTAGATCCTGATTATC

AGCCCTATACTGCATTTACTTTACCATCTAAGAATAATCAAAGTCCAGGAAAAAGGTATATTTGGAAATCTC

TTCCACAGGGGTGGGTCTTGAGTCCCTTAATATACCAGAGCACTCTAGATAATATTCTACAACCATTTAGAA

>Sequence 15

ATGTTTTCCGGTGGCGGCGGCCCGCTGTCCCCCGGAGGAAAGTCGGCGGCCAGGGCGGCGTCCGGGTTTTTT

GCGCCCGCCGGCCCTCGCGGAGCCGGCCGGGGACCCCCGCCTTGCTTGAGGCAAAACTTTTACAACCCCTAC

CTCGCCCCAGTCGGGACGCAACAGAAGCCGACCGGGCCAACCCAGCGCCATACGTACTATAGCGAATGCGAT

GAATTTCGATTCATCGCCCCGCGGGTGCTGGACGAGGATGCCCCCCCGGAGAAGCGCGCCGGGGTGCACGAC

GGTCACCTCAAGCGCGCCCCCAAGGTGTACTGCGGGGGGGACGAGCGCGACGTCCTCCGCGTCGGGTCGGGC

GGCTTCTGGCCGCGGCGCTCGCGCCTGTGGGGCGGCGTGGACCACGCCCCGGCGGGGTTCAACCCCACCGTC

ACCGTCTTTCACGTGTACGACATCCTGGAGAACGTGGAGCACGCGTAC

>Sequence 16

CTCGGGTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGGATCTGCCCGATAGAGGGGGATAACCACTGGAA

ACGGTGGCTAATACCGTATAACGTCGCRAGACCAAAGAGGGGGACCTTCGGGCCTCTCACTATCGGATGAAC

CCAKATGGGATTAGCTAGTRSGCGGGGTMACGGGCCCACCTAGGCGACKATCCCTAGCTGGTCTGAGAGGAT

GACCAGCCACACTGGAACTGASACACGGYCCASACTCCTACGGGRGGCAGCAGKGGGGAATATTGCACARTG

GGCGCAMGCCTGATGCASCCATGCCGYGTGTATGAAGARGGCCTTCGGGTTGTAAAGTWCTTTCAGCGGGGA

GGAAGGCKATGTGGTTAATAACCGCVTYGATTGACGTTACCCGCAGAAGAAGCACCGKCTAACTCCGTGCCA

GCAGCCGCGGTWATACGGAGGG

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