Exercise 6
Exercise 6
Exercise 6
Restriction Digestion
>Example gene
atgacagggtgggggatccagagcttatataaggatgcaatcgagggatccaaggcaataaaatcagttccaaaggttaaaggagttctcctaggctataacacaaacatagatgccataaaatacctagactctaaggatctcgaagggatccagagaatagagaaagtcggtaaggaggaagtaggatccttaaagtactccgaagaggatccgcttccagaaaaaatcacggatccttcaatcccgcagcttctcggttcaattctctggagcattaggagggatccgggcaaagctgcggagctatttgttgaaagttgtcctgtcagggatccattctatatgaagagatggggctggaatgggatccagctcaggaggatcctgggaggtcaggtagggataggatccatggccaacctcttaggtggagtttacggcgtgcctgttatagctcatgtcccccagatttcaaggttacaggcgagcctggatcccttcctcgatgggccaataggatcctacgtccccaaagttggatccgaagatggcaaggttaag- Open DNA cutter using following link
http://heimanlab.com/cut2.html
- Analyze restriction sites
- How many restriction sites are present
- Which are signal cutter (unique cutter) ?
- Which are multi cutter enzymes ?
- Search a gene of your choice and digest it with BamHI and EcoRI ? show results