Translate DNA sequence into protein
> Example Sequence
atgacagggtgggagagcttatataaggatgcaatcgagaaggcaataaaatcagttccaaaggttaaaggagttctcctaggctataacacaaacatagatgccataaaatacctagactctaaggatctcgaagagagaatagagaaagtcggtaaggaggaagtattaaagtactccgaagagcttccagaaaaaatcacttcaatcccgcagcttctcggttcaattctctggagcattaggaggggcaaagctgcggagctatttgttgaaagttgtcctgtcagattctatatgaagagatggggctggaatgagctcaggatgggaggtcaggtagggataatggccaacctcttaggtggagtttacggcgtgcctgttatagctcatgtcccccagatttcaaggttacaggcgagcctcttcctcgatgggccaatatacgtccccaaagttgaagatggcaaggttaag
- Search “ExPASy translate tool” in google or click the following link
https://web.expasy.org/translate/
- copy the sequence, and then paste it into the translate sequence window in the ExPasy link.
- Under Output format select "Compact". This gives the amino acid sequence as one letter codes with stop codons indicated by a hyphen.
- Click on Translate Sequence
- Often only one reading frame will give you a translation with no stop codons, but this is not always the case. If you get multiple possible reading frames, one way to determine which is most likely the true frame is to use the BLAST program (you will use it in next exercises) to determine if the sequence corresponds to any known protein sequence.
- Using the "Compact output" to get one letter sequences, copy the one letter sequence of the best reading frame (i.e. one with no stop codons) and paste it into the word file you will make and label it as "Best Guess".
- Copy the longest amino acid sequence (i.e. no hypens) of one of the other reading frames to the file and label it as "Second Best". If you have two reading frames without a stop codon, simply copy each to the boxes below.