Coral and Sea Anemone Studies (2010-present)

Chuang PS, Kao WC, Yu ZR, Lin TC, Shih KN, Yang WY, Leung YY, Hsu MT, Chen TY, Shikina S*, Tang SL*, Cheng JY*. Innovative chips for coral cultivation: Enhanced flexibility and experimental applications. Methods in Ecology and Evolution, 16, 707–714. 2025. *Corresponding authors

Shikina S*, Tsai PH, Chiu YL, Chang CF. The stony coral Fimbriaphyllia (Euphyllia) ancora's reproductive strategy involves a sex change every year. Communications Biology, 7. 2024 *Corresponding author

Shikina S*, Yoshioka Y, Chiu YL, Uchida T, Chen E, Cheng YC, Lin TC, Chu YL, Kanda M, Kawamitsu M, Fujie M, Takeuchi T, Zayasu Y, Satoh N, Shinzato C*. Genome and tissue-specific transcriptomes of the large-polyp coral, Fimbriaphyllia (Euphyllia) ancora: a recipe for a coral polyp. Communications Biology, 7,899. 2024. *Corresponding authors

Shikina S*, Cheng YC, Lin TC, Chang YE, Tsai PH, Chen E, Chang YC, Leung YY, Yu YC, Chiu YL. Coral mariculture using abandoned abalone farming ponds in northeastern Taiwan. Aquaculture, 592, 740872. 2024. *Corresponding author

Chuang PS, Wang TH, Lu CY, Tandon K, Shikina S, Tang SL*. Microbiome heterogeneity in tissues of the coral, Fimbriaphyllia (Euphyllia) ancora. Environ Microbiol Rep. 16, e13310, 2024.  

Hsieh YE, Lu CY, Liu PY, Kao JM, Yang SY, Wu CY, Wong JWM, Shikina S, Fan TY, Yang SH. Successive responses of three coral holobiont components (coral hosts, symbiotic algae, and bacteria) to daily temperature fluctuations. Ecological Indicators, 158, 111515. 2024

Shao PJ, Chiu YL, Tsai PH, Shikina S*. Possible germline progenitor cells in extra-gonadal tissues of the sea anemone, Exaiptasia diaphana. Frontiers in Marine Science, 10, 1278022. 2023. *Corresponding author.

Shikina S*, Lin TC, Chu YL, Cheng YC, Chang YE, Wada N, Tang SL, Iizuka Y, Chiu YL. Culturing reef-building corals on a laboratory dish: a simple experimental platform for stony corals. Frontiers in Marine Science, 10, 1149495. 2023. *Corresponding author.

Shikina S*, Chiu YL, Chung YJ, Yao YC, Liu TY, Lee YH, Mita M, Chang CF. Cellular and Biochemical Changes in Early Embryonic Development of a Scleractinian Coral, Fimbriaphyllia (Euphyllia) ancora. Zoological Studies, 62: 38. 2023. *Corresponding author.

Chan YF, Chen CY, Lu CY, Tu Y, Tandon K, Shikina S, Tang SL. First insight into the heat-induced changes in proteomic profiles of the coral symbiotic bacterium Endozoicomonas montiporae. Frontiers in Marine Science, 9: 808132. 2022.

Chiu YL, Chang CF, Shikina S*. Development of an in vitro tissue culture system for hammer coral (Fimbriaphyllia ancora) ovaries. Scientific Reports,11: 24338. 2021. *Corresponding author.

Zhang Y, Shikina S*, Ho Y, Chiu YL, Yao YC, Zatylny-Gaudin C, Dufor S, Chang CF. Involvement of RFamide neuropeptides in polyp contraction of the adult scleractinian corals Euphyllia ancora and Stylophora pistillata. General and Comparative Endocrinology, 285: 113270. 2021. *Corresponding author.

Chiu YL, Shikina S*, Yoshioka Y, Shinzato C*, Chang CF*. De novo transcriptome assembly from the gonads of a scleractinian coral, Euphyllia ancora: molecular mechanisms underlying scleractinian gametogenesis. BMC Genomics, 21: 732. 2020. *Co-corresponding author.

Shikina S, Chen CC, Chiu YL, Tsai PH, Chang CF. Apoptosis in gonadal somatic cells of scleractinian corals: Implications of structural adjustments for gamete production and release. Proceedings of the Royal Society B: Biological Sciences, 287: 2020578. 2020.

Shikina S*, Chiu YL, Zhang Y, Yao YC, Liu TY, Tsai PH, Zatylny-Gaudin C, Chang CF. Involvement of GLWamide neuropeptides in polyp contraction of the adult stony coral Euphyllia ancora. Scientific Reports, 10: 9427. 2020. *Co-corresponding author.

Chen CJ, Chen WJ, Shikina S, Denis V, Chang CF. The plasticity of gonad development of sexual reproduction in a scleractinian coral, Porites lichen. General and Comparative Endocrinology, 285: 113270. 2020.

 Zhang Y, Chiu YL, Chen CJ, Ho YY, Shinzato C, Shikina S*, Chang CF*. Discovery of a receptor guanylate cyclase expressed in the sperm flagella of stony corals. Scientific Reports, 9: 14652. 2019. *Co-corresponding author.

Chiu YL, Shikina S*, Chang CF*. Testicular somatic cells in the stony coral Euphyllia ancora express an endogenous green fluorescent protein. Molecular Reproduction and Development, 86: 798-811. 2019 *Co-corresponding author.

Shikina S, Chiu YL, Ye MR, Yao IC, Chung YJ, Chen CJ, Chang CF*. Molecular cloning and characterization of proliferating cell nuclear antigen (PCNA); a potential involvement of PCNA in germ cell development of stony corals. Fisheries Science, 84: 765-775. 2018. *Corresponding author.

Shikina S*, Chiu YL, Chen CJ, Yang SH, Yao JI, Chen CC, Wei JD, Shao ZF, Chang CF. Immunodetection of acetylated alpha-tubulin in stony corals: Evidence for the existence of flagella in coral male germ cells. Molecular Reproduction and Development, 84: 1285-1295. 2017. *Co-corresponding author.

Shikina S*, Chung YJ, Chiu YL, Huang YJ, Lee YH, Chang CF*. Molecular cloning and characterization of a steroidogenic enzyme, 17b-hydroxysteroid dehydrogenase type 14, from the stony coral Euphyllia ancora (Cnidaria, Anthozoa). General and Comparative Endocrinology, 228: 95-104. 2016. *Co-corresponding author.

Shikina S*, Chiu YL, Chung YJ, Chen CJ, Lee YH, Chang CF*. Oocytes express an endogenous red fluorescent protein in a stony coral, Euphyllia ancora: a potential involvement in coral oogenesis. Scientific Reports, 6: 25868. 2016.*Co-corresponding author.

Chen CJ, Shikina S*, Chen WJ*, Chung YJ, Chiu YL, Bertrand JAM, Lee YH, Chang CF*. A novel female-specific and sexual reproduction associated Dmrt gene discovered from a stony coral, Euphyllia ancora (Cnidaria, Anthozoa). Biology of Reproduction, 94 :40, 1-13. 2016. *Co-corresponding author.

Shikina S*, Chiu YL, Lee YH, Chang CF*. From somatic cells to oocytes: a novel yolk protein produced by ovarian somatic cells in a stony coral, Euphyllia ancora. Biology of Reproduction, 93: 57, 1-10. 2015. *Co-corresponding author.

Shikina S*, Chung YJ, Wang HM, Chiu YL, Shao ZF, Lee YH, Chang CF*. Immunohistochemical localization of early germ cells in a stony coral, Euphyllia ancora: potential implications for a germline stem cell system in coral gametogenesis. Coral Reefs, 34: 639-653. 2015. *Co-corresponding author.

Shikina S, Chen CJ, Chung YJ, Shao ZF, Liou JY, Tseng HP, Lee YH, Chang CF*. Yolk formation in a stony coral Euphyllia ancora (Cnidaria, Anthozoa): insight into the evolution of vitellogenesis in non-bilaterian animals. Endocrinology, 154: 3447-3459. 2013. *Corresponding author.

Shikina S, Chen CJ, Liou JY, Shao ZF, Chung YJ, Lee YH, Chang CF*. Germ cell development in the scleractinian coral Euphyllia ancora (Cnidaria, Anthozoa). PLoS ONE, 7: e41569. 2012. *Corresponding author.

Book Chapters (Invited)

Shikina S*, Chang CF. Neuropeptides as potentiators of coral polyp contraction. In: Saleuddin S, Leys SP, Roer R, Wilkie L (eds.), Frontiers in Invertebrate Physiology: A Collection of Reviews, Volume 1, Non-Bilaterian Phyla. 2024. *Corresponding author.

Shikina S*, Chang CF*. Cnidaria. A chapter in Encyclopedia of Reproduction. Second edition. Skinner M (ed). Elsevier. 2018. *Co-corresponding author.

Shikina S*, Chang CF*. Sexual reproduction in stony corals and insight into the evolution of oogenesis in Cnidaria. A chapter in The Cnidaria, past, present and future: The world of Medusa and her sisters. Stefano Goffredo S, Dubinsky Z (eds). Chapter 16. pp249-268. Springer. 2016. *Co-corresponding author.

Articles in Japanese (Invited) 

識名信也*. 生態学の知見を取り入れたサンゴ礁保全再生のアプローチ. Ocean Newsletter: No.576. 4-5. 2024. (笹川平和財団　海洋政策研究所)

識名信也*. イシサンゴの配偶子形成に関する研究: Study on gametogenesis in stony corals. Nippon Suisan Gakkaishi,87: 327-330. 2021. *Corresponding author.

識名信也*、邱顗陵、張燕、姚毅前、柳泰宇、蔡品萱、Céline Zatylny-Gaudin、張清風. 神経ペプチド GLWamide はイシサンゴのポリプ収縮に関与する. 比較內分泌學. 46: 122-125. 2020. *Corresponding author.

識名信也*、張清風. 造礁サンゴの配偶子形成. 生物科学 (Biological Science).  67: 216-227. 2016. *Corresponding author.

識名信也*、張清風. サンゴの有性生殖：ナガレハナサンゴの配偶子形成と一斉産卵. 海洋と生物（Aquabiology）219: 419-425. 2015. *Co-corresponding author.

Articles in Chinese

識名信也, 謝東廷, 鄭尹筑, 楊仲家 探討電弧爐碴試體在半開放式的天然海域內對珊瑚之影響以及評估製成人工珊瑚魚礁的可行性. 

           混凝土科技, 17: 103-114. 2023.

識名信也, 謝東廷, 鄭尹筑, 楊仲家.以珊瑚養殖評估氧化碴及還原碴 再利用於海域環境之可行性研究. 混凝土科技, 16: 71-74. 2022.

黃康訓, 邱顗陵, 識名信也, 楊仲家. 水泥基質材料與珊瑚養殖的結合：建立具有珊瑚的混凝土. 混凝土科技, 14: 71-74. 2020.

Fish Germ Cell Studies at the Tokyo University of Marine Science and Technology (2006-present)

Iwasaki-Takahashi Y＃, Shikina S＃, Watanabe M, Bamba A, Yagisawa M, Takahashi K, Fujihara R, Okabe T, Valdez Jr DM, Yamauchi A, Yoshizaki G. Production of functional eggs and sperm from in vitro-expanded type A spermatogonia in rainbow trout. Communications Biology, 3: 308. 2020. ＃Co-first author.

Okutsu T, Shikina S, Sakamoto T, Mochizuki M, Yoshizaki G*. Successful production of functional Y eggs derived from spermatogonia  

        transplanted into female recipients and subsequent production of YY supermales in rainbow trout, Oncorhynchus mykiss. Aquaculture,   

        446: 298-302. 2015. 

Shikina S, Nagasawa K, Hayashi M, Furuya M, Iwasaki Y, Yoshizaki G*. Short-term in vitro culturing improves transplantability of type A spermatogonia in rainbow trout (Oncorhynchus mykiss). Molecular Reproduction and Development, 80: 763-773. 2013. 

Lee S, Iwasaki Y, Shikina S, Yoshizaki G*. Generation of functional eggs and sperm from cryopreserved whole testes. Proceedings of the National Academy of Sciences of the United States of America,110: 1640-1645. 2013. 

Yoshizaki G*, Fujinuma K, Iwasaki Y, Okutsu T, Shikina S, Yazawa R, Takeuchi Y. Spermatogonial transplantation in fish: A novel method for the preservation of genetic resources. Comparative Biochemistry and Physiology-Part D: Genomics and Proteomics, 6: 55-61. 2011. 

 Shikina S, Yoshizaki G*. Improved in vitro culture conditions to enhance the survival, mitotic activity, and transplantability of rainbow trout type A spermatogonia. Biology of Reproduction, 83: 268-276. 2010. 

Nagasawa K, Shikina S, Takeuchi Y, Yoshizaki G*. Lymphocyte antigen 75 (Ly75/CD205) is a surface marker on mitotic germ cells in rainbow trout. Biology of Reproduction, 83: 597-606. 2010.

Yoshizaki G*, Ichikawa M, Hayashi M, Iwasaki Y, Miwa M, Shikina S, Okutsu T. Sexual plasticity of ovarian germ cells. Development, 137: 1277-1230. 2010.

Shikina S, Ihara S, Yoshizaki G*. Culture conditions for maintaining the survival and mitotic activity of rainbow trout transplantable type A spermatogonia. Molecular Reproduction and Development, 75: 529-537. 2008. 

Okutsu T, Shikina S, Kanno M, Takeuchi Y, Yoshizaki G*. Production of trout offspring from triploid salmon parents. Science, 317: 1517. 2007. 

Sawatari E, Shikina S, Takeuchi T, Yoshizaki G*. A novel transforming growth factor-β superfamily member expressed in gonadal somatic cells enhances primordial germ cell and spermatogonial proliferation in rainbow trout. Developmental Biology, 301: 266-275. 2007. 

Okutsu T, Yano A, Nagasawa K, Shikina S, Kobayashi T, Takeuchi T, Yoshizaki G*. Manipulation of fish germ-cells: visualization, cryopreservation, and transplantation. Journal of Reproduction and Development, 52: 685-693. 2006.