關於我們（About Us）

實驗室簡介（Lab Overview）

Protein Folding and Ligand Binding Laboratory 專注於研究蛋白質摺疊與小分子結合機制，並探索其在食品科學、生物技術與醫學應用的潛力。我們運用蛋白質工程、結構生物學與生物物理技術，以解決與食品安全、藥物開發及功能性成分相關的科學問題。

The Protein Folding and Ligand Binding Laboratory studies the mechanisms of protein folding and small molecule binding, exploring their potential applications in food science, biotechnology, and medicine. We employ protein engineering, structural biology, and biophysical techniques to address scientific challenges related to food safety, drug development, and functional ingredient research.

Biochemical Techniques

Protein Purification

Heterologous expression of recombinant protein

Liquid chromatography

•Ion exchange chromatography

•Size exclusion chromatography

•Affinity chromatography

Protein Chemistry

Protein separation by 2-D gel electrophoresis

In-gel trypsin digestion and protein Identification by mass spectrometry

Protein crosslinking

Polyacrylamide gel electrophoresis

Limited proteolysis

Pulse proteolysis

Immunochemistry

Western blotting

Far western blotting

Immuno-precipitation

ELISA

Biophysical Techniques

Analysis of thermodynamic stability of proteins

Kinetic analysis of protein folding and unfolding

Thermodynamic analysis of complex formation

Isothermal titration calorimetry (ITC)

Spectroscopy

•UV spectroscopy

•NMR spectroscopy

•CD spectroscopy

•Fluorescence spectroscopy

Mass spectrometry

MALDI-TOF-TOF mass spectrometry

Molecular Biology Techniques

Site-directed mutagenesis

Ligation: Cohesive-ended, Blunt-ended, three way ligation

Restriction enzyme screening

Polymerase chain reaction

Electrophoresis of nucleic acids

Heat shock transformation

Electroporation

Competent cell preparation