Paper : XIV

Microbial Fermenation Technology (MFT) PG- MB (08)- S4-T2

Name of the Teacher : Dr. Bhavana V. Mohite

Zoom online lecture:

Join Zoom Meeting, Recurring Meeting

https://us04web.zoom.us/j/74145329697?pwd=T2pPbHh3S2NNWjhVckZGWWp5Wm1IQT09

Meeting ID: 741 4532 9697

Passcode: 7uBsxV

Day 1: 24 th March 2021, 3.00 pm : Introduction to Syllabus

Unit I

Day 2: 25th March 2021, 3.00 pm : Types of Bioreactor 1

Day 3: 26th March 2021, 3.00 pm : Types of Bioreactor 2

Day 4:27th March 2021, 11.00 am :Immobilized Reactor & Types of Fermentation

Types of Fermentation 2

Day 5: 30th March 2021, 3.00 pm : Microbial Cell Growth

Day 6: 31st March 2021, 12.00 pm: Microbial Growth Kinetics 1

Day 7: 1st April 2021,12.00 pm: Monod Model 1

Day 8: 3rd April 2021, 11.00 am Monod Model 2

Day 9: 5th April 2021, 3.00pm Stringent Response, Ntr and Pho , Substrate accelerated Death

Day 10: 6th April 2021, 2.00 pm Growth Yield, Product formation

Day 11: 7th April 2021, 11.00 am Maintenance Energy and Yield coefficient

Day 12: 8th April 2021, 1.00 pm Process of Optimization

Day 13: 9th April 2021, 12.00 pm Statistical designs for optimization

Day 14: 10th April 2021, 10.00 am Rheology of fermentation Fluid

Day 15: 12th April 2021, 11.00 am Oxygen transfer and uptake

Day 16: 15th April 2021, 11.00 am Oxygen transfer kinetics

Day 17: 16th April 2021, 12.00 pm Factors affecting oxygen transfer

Day 18: 17th April 2021, 10.30 am Determination of OTR and OUR

Assignment 1: Bioreactor (Last Date 21th April 2021)

Day 19: 19th April 2021, 2.00 pm (Chemostat and Turbidostat)

Unit Test 1: 5th May 2021, 4.30 pm

Unit II

Day 20: 20th April 2021, 11.00 am (Downstream processes)

Day 21: 21st April 2021, 3.00 pm (Methods of recovery)

Day 22: 22nd April 2021, 1.00 pm (Storage and Packaging)

Day 23: 24th April 20201, 10.00 am (Scale Up Process)

Day 24: 26th April 2021, 11.00 am (Criteria for scale up, purpose, need)

Day 25: 27th April 2021, 11.00 am (Scale down Process)

Assignment 2 : Downstream Processing (Last Date: 5th May 2021)

Day 26: 28th April 2021, Productivity Power requirement Basic control theory 1

Day 27: 29th April 2021 Productivity Power requirement Basic control theory 2

Assignment 2: Downstream Processing and scale up (Last date 5th May 2021)

Unit Test 2: 22nd May 2021, 10.00 am

Unit III

Day 28: 30th April 2021, 2.00 pm Bioethanol as biofuel

Day 29: 3rd May 202, 3.00 pm Production of bioethanol

Day 30: 4th May 2021, 11.00 am Biohydrogen as Biofuel

Day 31: 6th May 2021, 11.00 am Biomethane production

Day 32: 7th May 2021, 11.30 am Penicillin structure, derivatives, mode of action

Day 33: 8th May 2021, 10.30 am Penicillin production and downstream processing

Day 34: 10th May 2021, 11.15 am Streptomycin Structure, phases of synthesis

Day 35: 11th May 2021, 1.30 pm Production and recovery of streptomycin

Day 36: 12th May 2021, 11.00 am Cephalosporin Structure, mode of action and fermentative production

Day 37: 13th May 2021, 11.00 am Lactobacillus sakei as biopreservative

Day 38: 15th May 2021, 10.00 am ; Genetics of L. sakei and Bacteriocin production

Day 39: 17th May 2021, 11.15 am Xanthun gum production

Day 40: 18th May 2021, 11.00 am Xanthun gum structure, properties and applications

Day 41: 19th May 2021, 3.00 pm PHA History Structure & properties

Day 42: 20th May 2021, 12.00 pm PHA biosynthesis , production

Day 43: 21st May 2021, 11.00 am PHA: fermentation , recovery and applications

Day 44: 24th May 2021, 1.30 pm Protease structure classification and screening

Day 45: 25th May 2021, 11.00 am Thermostable proteases Fermentation recovery and applications

Day 46: 28th May 2021, 2.00 pm Biosurfactant Types, properties, production and applications

Day 47: 29th May 2021, 10.00 am Biosurfactant Comparative account

Assignment 3: Industrial Fermentation Product (Last Date 5th June 2021)

Unit IV

Day 48: 31st May 2021, 11.00 am SCP Introduction and Types microorganisms involved

Day 49: 1st June 2021, 11.00 am SCP Nutritional value and types

Day 50: 2nd June 2021, 2.00 pm SCP Types and Process

Day 51:3rd June 2021, 3.00 pm Carotenoid structure, properties and Types

Day 52: 5th June 2021, 11.00 am Carotenoid production

Day 53: 8th June 2021, 11.00 am Lysine production

Day 54:9th June 2021, 11.00 am Glutamic acid production

Day 55:11th June 2021, 11.00 am Vit B2 Production process

Day 56:9th June 2021, 9.30 am Vit B12 production , Palmetate and Olieate production

e books

Principles of fermentation technology by Stanbury and Whitekar

Industrial Microbiology: An introduction by Michael J Waites et al

Study Material

Unit I

Study Material on Growth Kinetics and Growth Phases

Study Material on Growth Kinetics 1

Study Material on Process Kinetics

Study Material on Substrate Accelerated Death

Study Material on Stringent Response: Ntr and Pho s

Study Material on Ntr and Pho System 2

Study Material on Rheology of Fermentation Fluid

Study Material on Oxygen Transfer Kinetics

Study Material on Oxygen Transfer Kinetics part 2

Study Material on Monod Kinetics

Study Material on Optimization Process

Study Material on Chemostat and Turbidostat

Unit 2

Study Material on Packaging and storage

Study Material on Packaging and storage methods 2

Study material on downstream processes

Recovery and purification of fermentation products

Scale up process, Criteria for scale up

Scale up and Scale down process

Difference between scale up and Scale down

Study Material on Productivity Power requirement Basic control theory

Unit 3

Online Study Material on Biofuel

Study Material on Biofuel: Bioethanol, Biohydrogen, Methane

Study Material on Penicillin

Online study material on Penicillin structure, mode of action and synthesis

Online study material on Penicillin production and recovery

Study Material on Streptomycin production

Study Material on Streptomycin production part 2

Study Material on Cephalosporin

Study Material on Fermentation of cephalosporin

Study Material on Cephalosporin structure, mode of action and biosynthesis

Online study material on Lactobacillus sakei as biopreservative

Study Material on Genetics of L. sakei and bacteriocin production

Online study Material on Xanthan gum production

Online study material on Xanthan gum structure, properties

Study Material on Xanthan Gum Applications

Study Material on PHA structure and biosynthesis

Study Material on PHA production

Study Material on PHA fermentation, recovery and applications

Online Study Material on Proteases structure, classification and screening

Online Study Material on Biotechnological aspects of microbial Proteases

Study Material on Thermostable Proteases fermentation and recovery and applications

Online Study material on Biosurfactant Types, properties, production and applications

Online Study material on Biosurfactant Production and applications

Study material on Biosurfactant Comparative account

Unit 4

Study Material on SCP Types, microorganisms involved

Study Material on SCP types and process

Study Material on Carotenoid Properties and Types

Study Material on Carotenoid production Study Material on Vit B2

Review on Carotenoid production

Study Material on Production of Lysisne 1

Study Material on Production of Lysine 2

Study Material on Production of Glutamic acid

Study Material on Fermentation process of Vit B2

Study Material on Vit B12

Study Material on VIt B12 production

Study Material on Palmetate and Oleate

VACCINES AND DELIVERY SYSTEM (VD)

Nmae of the teacher:Mr. M.G. Ingale

Links for Ebook & Study materials:

1. Vaccines by Plotkin

2. Immunology by Kuby

3. Molecular biology & biotechnology by John M. walker

4. Microbial Biotechnology - Fundamentals of Applied Microbiology (Glazer & Nikaido)

Links for study materials:

UNIT-I: - Vaccines

1. Definition and discovery of vaccines

2. Active and passive prophylactic measures

3. Exaltation & attenuation

4. Subunit vaccines & DNA vaccines

5. Vaccines additives and adjuvants

UNIT-II: - Conventional vaccines

1. Study material: BCG Vaccine

2. Study material: DPT vaccine

3. Study material: Polio vaccine (Inactivated, live attenuated)

4. Study material: Hepatitis vaccine

5. Study material: Influenza vaccine

6. Study material: MMR vaccine

UNIT-III: - Vaccines in development

1. Study material: Malaria vaccine

2. Study material: Epstain Barr virus vaccines

3. Study material: Cytomegalo virus vaccines

4. Study material: HIV vaccines

5. Study material: Herpes simplex viral vaccines

UNIT-IV: - Designing & delivery system.

1. Drug designing

2. Non-automated in vitro drug susceptibility testing

3. Rapid tests for susceptibility testing

4. Antibiotic assay in body fluid

5. Drugs delivery system.

6. Vaccines delivery system.

Links for video lecture:

UNIT-I: - Vaccines

1. Video lecture: Vaccines Part 1

2. Video lecture: Vaccine Part 2

3. Video lecture: Discovery of vaccines

4. Video lecture: DNA vaccine & Subunit vaccine

5. Video lecture: Exaltation & attenuation

6. Video lecture: Vaccines additives and adjuvants

UNIT-II: - Conventional vaccines

1. Video lecture: BCG Vaccine

2. Video lecture: Polio vaccine

UNIT-IV: - Designing & delivery system

1. Video lecture: Drug designing part 1

2. Video lecture: Drug designing part 2

3. Video lecture: Non-automated in vitro drug susceptibility testing part 1

4. Video lectures: Non-automated in vitro drug susceptibility testing part 2

5. Video lectures: Rapid tests for susceptibility testing

6. Video lecture: antibiotic assay in body fluid

7. Video lectures: Drugs delivery system part 1

8. Video lecture: Drugs delivery system part 2

9. Video lecture: Drugs delivery system part 3

10. Video lecture: Drugs & vaccines delivery system part 4

11. Video lecture: Vaccines delivery system part 1

12. Video lecture: Vaccines delivery system part 2

VIROLOGY

Unit 3 Animal and Plant Viruses

Dr. B. V. Mohite

Video lecture on Tobacco Mosaic Virus

Video lecture on Tobacco Mosaic Virus 2

Study Material on TMV 1

Study Material on TMV 2

Study Material on TMV Symptoms, Multiplication and control

Video lecture on Cauliflower Mosaic Virus

Study Material on CMV

Study Material on CMV 2

Study Material on CMV Genome, replication and Application

Online Mini review on CMV

Video lecture on Potato virus

Study Material on Potato virus Types, Symptoms and Control

Study Material on Potato virus structure and genome

Study Material on Potato virus

Study Material on Potato leaf roll over virus

Dr. S .S. Khandare:

Link for Video lectures:

1. Pox virus part 1

2. Pox virus part 2

3. Herpes virus

drive.google.com/file/d/1vVwIbQ8LycKwt2SoiLDkf1znzvDpTf4Z/view?usp=sharing

4.Adenovirus part 1.

drive.google.com/file/d/1dqP8s99kyOtRlrzDWMLbYZq5WPXHXJsO/view?usp=sharing

5. Adenovirus part 2.

drive.google.com/file/d/15qu5cFNwKl1L9Z_ZA2FkEPUZn5XN2c0j/view?usp=sharing

Mr. M. G. Ingale

Unit IV: General methods of Diagnosis and antiviral drugs

Ebook: Virology by John Carter

Study material:

1. Antiviral agents (Amantadine, Rimantidine,Vidarabine, Acyclovir,Ganciclovir,Ribavirin, Foscarnet)

2. Nucleoside RT inhibitors, non-nucleoside RT inhibitors (NNRTIS) & Protease inhibitors

3. Types of IFN and Molecular basis of antiviral effect of interferon

PRACTICAL-VII

MB4-LAB 7

LABORATORY EXERCISE 7

1. Isolation of viruses (bacteriophage) from water sources

2. Microbiological examination of food

3. Immobilization of cells/Enzymes

Practical Video links:

1. Isolation of viruses from water sources.

2. Video: Immobilization of yeast

3. Video: Microbiological examination of foods