M.Sc II Sem-III
Paper : X
Recombinant DNA technology and Nano Biotechnology (RDTN) MB3-T010
UNIT-I : - Molecular Cloning Methods (Miss. Pooja D. Bhoge)
Link for e-book (Click on Name of the book)
Day-1 : (21 September 2020) Monday
Topic : Restriction enzymes
-Video lecture by Miss. P. D. Bhoge : Click here
-Study material : Restriction enzymes
Day-2 : (22 September 2020) Tuesday
Topic: Cloning Vectors
-Study material : Cloning vector part-1
-Online Animation on Restriction enzyme : EcoR1
Day-3 : (23 September 2020) Wednesday
Topic: Types of Cloning Vectors
-Video lecture by Miss. P. D. Bhoge : Cloning vector part-1
-Online Animation on : Recombinant DNA technology Process
Day-4 : (24 September 2020) Thursday
-Study material : Cloning Vector part-2
-Online Animation on : Cloning vector Plasmid
Day-5 : (25 September 2020) Friday
Topic: Examples of Cloning Vectors
-Video lecture by Miss. P. D. Bhoge : Cloning vector part-2
-Online Animation on : M13 Phage vector
Day-6 : (29 September 2020) Tuesday
Topic: Genomic library
-Online Animation on : DNA Libraries
-Study material : Genomic library
Day-7 : (30 September 2020) Wednesday
-Video lecture by Miss. P. D. Bhoge : Genomic library
Day-8 : (1 October 2020) Thursday
Topic: cDNA library
-Study material : cDNA library
-Video lecture by Miss. P. D. Bhoge : cDNA Library_Part-1
Day-9 : (3 October 2020) Saturday
-Video lecture by Miss. P. D. Bhoge : cDNA Library_Part-2
-Online Animation on : cDNA Library
Day-10 : (5 October 2020) Monday
Topic: Screening and Identification of genes
-Video lecture by Miss. P. D. Bhoge : Screening & Identification of genes
-Online Animation on : Blue White Screening
Day-11 : (6 October 2020) Tuesday
-Online Animation on : Colony hybridization Screening method
Day-12 : (7 October 2020) Wednesday
-Study material : Screening & Identification of genes
Day-13: (8 October 2020) Thursday
-Study material : Heterologous probes and Oligonucleotide probes
Day-14: (13 October 2020) Tuesday
Topic: Microarrays
-Microarray and its application
Day-15: ( 14 October 2020) Wednesday
- Microarrays and Comparative Genomic Hybridization Microarrays work
-Study material : Microarray
Day-16: ( 15 October 2020) Thursday
-Video lecture by Miss. P. D. Bhoge : Microarrays_Part-1
-Online Animation on : DNA Microarray
Day-17: ( 16 October 2020) Friday
-Video lecture by Miss. P. D. Bhoge : Microarrays_Part-2
- e-notes on: DNA Microarrays
Day-18: ( 17 October 2020) Saturday
-Study material: Polymerase Chain Reaction (PCR)
Day-19: ( 19 October 2020) Monday
-Online Animation on: PCR
-Video lecture by Miss. P. D. Bhoge : PCR_Part-1
Day-20: ( 20 October 2020) Tuesday
-Study material: RT-PCR
-Online Animation on: RT-PCR
Day-21: ( 21 October 2020) Wednesday
-Video lecture by Miss. P. D. Bhoge : PCR_Part-2
UNIT II: Other molecular tools for studying genes
Name of the teacher: Dr. Abhinav Raut
Name of the Teacher : Dr. Bhavana V. Mohite
Zoom online lecture:
Join Zoom Meeting, Recurring Meeting
https://us04web.zoom.us/j/74145329697?pwd=T2pPbHh3S2NNWjhVckZGWWp5Wm1IQT09
Meeting ID: 741 4532 9697
Passcode: 7uBsxV
Unit II Other molecular tools for studying Gene Expression
Day 1: 27th February 2021, 11.00 am: Restriction mapping: DNA sequencing dideoxy
Day 2: 1st March 2021, 11.00 am: Pyrosequencing, DNA fingerprinting.
1st March 2021, 3.00 pm: S1 Mapping, primer extention
Day 3: 2nd March 2021, 3.00 pm: Dnase footprinting, DMS footprinting.
Day 4: 3rd March 2021, 4.30 pm: Nuclear run on transcription,
Day 5: 4th March 2021, 3.00 pm: reporter gene transcription.
Unit II Other molecular tools for studying Gene Expression
Day 6: 5h March 2021, 12.00 pm: Tissue plasminogen activator [TPA].
5h March 2021, 3.00 pm: Tissue growth factor B. Dnase; PDGF
Day 7: 6h March 2021, 9.00 am: Transgenic plants and plant products, Comparative account
Study Material
Unit II
Online Study Material on Restriction Mapping
Online Study Material on Restriction enzyme action and Mapping
Online Study Material on DNA sequencing
Online Study Material on DNA Sequencing and its types
Study Material on Dideoxy Sequencing method
Online Study Material on Pyrosequencing
Study Material on Pyro -Sequencing
Online study Material on DNA Fingerprinting
Online Study Material on DNA Footprinting
Study Material on DNAse Footrprinting
Study Material on DNA and DMS Footprinting
Online Study Material on DMS and DNAse Footprinting
Online Study Material on Primer extension and Nuclease S1 Mapping
Study Material on Primer extension
Online Study Material on Nuclease S1 Mapping of RNA
Online study material on Nuclease S1 Mapping
Online Study Material on Nuclear run on Assay
Study Material on Nuclear run on assay
Study Material on reporter gene transcription
Unit IV
Online Study Material on GM crops
Study Material on Transgenic plant and plant products
Online Study Material on Transgenic plant Development
Study Material on Recombinant Product TPA
Online study Material on recombinant TPA
Online Study Material on Recombinant Growth Factors
Link for Reference e- books
Principles of Gene Manipulation by Primrose et al 6th edition
Paper : XI
Microbial Diversity, Evolution and Ecology (MDEE)-1 MB3-T011
Day 6 : 28 September 2020
Topic : Primitive Life forms
Video lecture by Miss. Nisha T. Satone : click here
Study material : click here
UNIT-I : - Microbial Evolution and Systematics (Miss. Pooja D. Bhoge)
Link for e-book (Click on Name of the book)
Day-22: ( 22 October 2020) Thursday
-Topic : Endosymbiosis
-Study material : Endosymbiosis
-Online animation on: Click here
Day-23: ( 23 October 2020) Friday
-Topic : Methods for determining evolutionary relationships
-Study material : Methods for determining evolutionary relationships
Day-24: ( 24 October 2020) Saturday
-Video lecture by Miss. P. D. Bhoge : Evolutionary chronometers
UNIT-II: -Microbial Diversity: Archaea
Study material:
Paper : XII
Drugs and Disease Management (DDM) MB3-T012
Name of the teacher: Mr. M.G. Ingale
UNIT -I: Drug latentiation and Prodrug:
Lecture 1:
Mayur Ingale is inviting you to a scheduled Zoom meeting.
Topic: Concept of Drug latentiation and Prodrug
Time: Oct 17, 2020 11:00 AM India
Join Zoom Meeting
https://us04web.zoom.us/j/74033579058?pwd=Tkx1akNoSjgweDFMZmRjT1dTNmVIZz09
Lecture 2:
Mayur Ingale is inviting you to a scheduled Zoom meeting.
Topic: Approaches in Prodrug
Time: Oct 20, 2020 12:00 PM India
Join Zoom Meeting
https://us04web.zoom.us/j/74990761871?pwd=YTYyN3FnYzB3T1lIaVZwcmp1L3VWUT09
Lecture 3:
Mayur Ingale is inviting you to a scheduled Zoom meeting.
Topic: History of Prodrugs
Time: Oct 23, 2020 12:00 PM India
Join Zoom Meeting
https://us04web.zoom.us/j/73829564863?pwd=SXBoTEs4cVpSSHU5aWx6Q1ZHSWg3UT09
Lecture 4:
Mayur Ingale is inviting you to a scheduled Zoom meeting.
Topic: Types of Prodrug
Time: Oct 29, 2020 04:00 PM India
Join Zoom Meeting
https://us04web.zoom.us/j/75964396669?pwd=MVpmTmJoN1BVZnBNNldpMS9WN0RGdz09
Lecture 5:
Mayur Ingale is inviting you to a scheduled Zoom meeting.
Topic: Types of Prodrug
Time: Oct 31, 2020 11:30 AM India
Join Zoom Meeting
https://us04web.zoom.us/j/75964396669?pwd=MVpmTmJoN1BVZnBNNldpMS9WN0RGdz09
Lecture 6:
Mayur Ingale is inviting you to a scheduled Zoom meeting.
Topic: Prodrugs of amines & carboxyl compounds
Time: Nov 6, 2020 02:30 PM India
Join Zoom Meeting
https://us04web.zoom.us/j/76350687156?pwd=c09peTZLaURIelBxNDF2Q0pNYmdLdz09
Lecture 7:
Mayur Ingale is inviting you to a scheduled Zoom meeting.
Topic: Mechanism of drug action
Time: Nov 21, 2020 09:00 AM India
Join Zoom Meeting
https://us04web.zoom.us/j/75573860679?pwd=Nm5DcGxrU29yZUExZXdLVWk5MjRMUT09
Lecture 8:
Mayur Ingale is inviting you to a scheduled Zoom meeting.
Topic: Mechanism of drug action
Time: Nov 23, 2020 01:00 PM India
Join Zoom Meeting
https://us04web.zoom.us/j/76304301237?pwd=Vkl5YW41VXh2enh3RERJdjFLZjBZdz09
Lecture 9:
Mayur Ingale is inviting you to a scheduled Zoom meeting.
Topic: Mechanism of drug resistance
Time: Nov 24, 2020 11:30 AM India
Join Zoom Meeting
https://us04web.zoom.us/j/74052146891?pwd=MVJyc2tqNmNxZ0w3eEVEOTcwZGpsdz09
Lecture 10:
Mayur Ingale is inviting you to a scheduled Zoom meeting.
Topic: Mechanism of Multidrug resistance)
Time: Nov 25, 2020 02:30 PM India
Join Zoom Meeting
https://us04web.zoom.us/j/73499995419?pwd=blhvOVFkUkFTQ3p6UGhNVXhiT1NNdz09
Links for study material:
Links for Books:
Assignments:
Topic: Explain in detail concept of prodrugs & history of prodrugs (Last date of submission is 8/11/2020)
UNIT-II:
Day 1: 21 September 2020
Topic : Antiinfective agents
Video lecture by Miss. Nisha T. Satone : Click here
Day 2: 22 September 2020
Topic : Antiinfective agents- Iodophore
Study material : Antiinfective agents
DAY 3 : 23 September 2020
Topic: Antiinfective Agents - Benzalkoniun Chloride
Video lecture by Miss. Nisha T. Satone :Click here
Study material : Click here
Day 4 : 24 September 2020
Topic: Antiinfective Agents - Gentian Violet
Video lecture by Miss. Nisha T. Satone : click here
Study material : click here
Day 5 : 25 September 2020
Topic: Antiinfective Agents - Mercury Compound
Video lecture by Miss. Nisha T. Satone :click here
study material : click here
Antifungal agents:
Links for Study material:
Antitubercular agents
UNIT-III:
UNIT-IV: Histamines and Antihistaminic agents:
Study Material
PPT : Histamines and Antihistaminic agents: Cimetidine, Ramitidine, Omeprazole.
Analgesic agents: Morphine and their derivatives
PPT: anti-inflammatory analgesics- Phenylbutazone and oxyphenbutazone, Prostaglandins.
M.Sc sem III Practical soft copies (For practical Examination):
PRACTICAL-V MB3-LAB 5:
PRACTICAL-VI MB3-LAB 6:
Paper : IX
Molecular Biology and Genetics (MBG) MB3-T009
Name of the Teacher : Dr. Bhavana V. Mohite
Paper IX Syllabus Molecular Biology and Genetics (MBG)
Zoom online lecture:
Join Zoom Meeting, Recurring Meeting
https://us04web.zoom.us/j/74145329697?pwd=T2pPbHh3S2NNWjhVckZGWWp5Wm1IQT09
Meeting ID: 741 4532 9697
Passcode: 7uBsxV
Day 1: 14th October 2020, 12.00 pm : Introduction to Syllabus
Unit I: Replication, Repair and Recombination
Day 2: 16th October 2020, 3.00 pm: Replication: Basics and steps of replication
Day 3: 21th October 2020, 12.00 pm: Mechanism and Enzymes involved in replication
Day 4: 26th October 2020, 12.00 pm: Steps of replication and Initiation of replication in Eukaryotes
Day 5: 29th October 2020, 12.00 pm: Eukaryotic DNA replication, elongation and termination
Day 6: 5th November 2020, 12.00 pm DNA Repair Direct repair: Base excision repair, Nucleotide excision reapir
Day 7: 6th November 2020, 12.00 pm Mismatch repair, Transposons
Day 8: 20th November 2020, 12.00 pm RecBCD pathway
Day 9: 21st November 2020, 12.00 pm Gene Conversion
Unit Test on Replication, Recombination and Repair [16th February 2021, 5.00 pm]
Unit III Gene Regulation Expression
Day 10: 27th November 2020, 12.00 pm Lactose Operon
Day 11: 28th November 2020, 12.00 pm Tryptophan Operon
Day 12: 2nd December 2020, 2.00 pm Arabinose Operon
Day 13: 9th December 2020, 2.00 pm Chromatin Remodelling
Day 14: 10th December 2020, 2.00 pm Animations on Different Operon
Lactose Operon
https://www.youtube.com/watch?v=g9GvmofU6jQ
https://www.youtube.com/watch?v=bZNEzeWHakU
Tryptophan Operon
https://www.youtube.com/watch?v=4pSTNYqQvXM
https://www.youtube.com/watch?v=PehrFpmaGKo
Arabinose Operon
https://www.youtube.com/watch?v=l0Vtb350vWA
https://www.youtube.com/watch?v=Djejj8smn3w
Day 15: 17th December 2020, 2.00 pm
Day 16: 18th December 2020, 3.00 pm
Day 17: 21st December 2020, 2.00 pm
Unit Test on Gene Regulation Expression [20th February 2021, 2.15 pm]
Unit IV Genetics of Bacteria and Bacteriophages
Day 18: 18th January 2021, 2.00 pm: Gene mapping by Conjugation
Day 19: 19th January 2021, 2.00 pm: Gene mapping by Transformation and Transduction
Day 20: 22nd January 2021, 11.00 am: Mapping of Bacteriophage gene- Introduction
Day 21: 23nd January 2021, 10.00 am: Mapping of Bacteriophage gene- Deletion Mapping
Day 22: 25th January 2021, 2.00 pm: Mapping of Bacteriophage gene- Recombinational analysis
Day 24: 1st February 2021, 12.00 pm: Mapping of Bacteriophage gene- Complementation
Day 25: 2nd February 2021, 12.00 pm: Transposons: Insertion elements
Day 26: 3rd February 2021, 12.00 pm: : Transposons: Insertion elements
Day 26: 4th February 2021, 3.00 pm: Replicative and Non Replicative Transposon
Day 26: 5th February 2021, 3.00 pm: Composite Transposon
Day 27: 6th February 2021, 9.30 am: P elements
Day 28: 8th February 2021, 12.00 pm: Retrotransposon
Day 29: 10th February 2021, 3.00 pm: Review of the Unit IV
Unit Test on Genetics of Bacteria and Bacteriophages [1st March 2021, 5.00 pm]
Unit II Gene Expression
Day 30: 11th February 2021, 12.00 pm: Transcription : Introduction
Day 31: 12th February 2021, 12.00 pm: Comparative study of prokaryotic and eukaryotic transcription process
Day 32: 13th February 2021, 9.30 am: Class I, II, III RNA Polymerase
Day 33: 15th February 2021, 3.00 pm: Promoters, Enhancers and silencers
Day 34: 16th February 2021, 12.00 pm:rs, General and specific transcription factors
Day 35: 17th February 2021, 3.00 pm:mRNA, rRNA and tRNA processing
Day 36: 18th February 2021, 12.00 pm: Splicing mechanism
Day 37: 20th February 2021, 9.00 am: RNA editing, post transcriptional control of gene expression
Day 37: 23rd February 2021, 3.00 pm: Gene Silencing by RNA interference
Day 38: 24th February 2021, 12.00 pm: Catalytic RNA, Antisense RNA
Day 39: 25th February 2021, 12.00 pm: Translation- Steps
Day 40: 26th February 2021, 12.00 pm: Post Translational Modification
Link for e- books
Molecular biology of the Gene by Watson, Baker et al.
Cell biol 2007 Molecular cell biology 6th ed Harvery lodish et al
Study Material
Unit I
Overview of Replication, enzymes, mechanism and steps
Study Material on DNA Replication
Online E PG Pathshala Eukaryotic DNA Replication
Online Study Material on DNA Replication
Online study Material on DNA Repair 1
Online Study Material on DNA repair 2
Study Material on Excision repair and Double strand break repair
Study Material of e PG Pathshala on DNA repair 1
Study Material of e PG Pathshala on DNA Damage and repair 2
Study Material on Homologous Recombination RecBCD pathway
Study Material on Gene Conversion
Unit II
Study Material on Lactose Operon
Study Material on Tryptophan Operon
Study Material on Arabinose Operon
Study Material on Chromatin Remodelling 1
Online Study Material on Chromatin Remodelling 2
Assignment 1
Assignment 1: DNA Replication [Due Date: 09/11/2020]
Unit III
Study Material on Introduction and summary of Transcription
Study Material on Transcription and Translation
Study Material on Protein Synthesis
Study Material on Prokaryotic Transcription
Study Material on Eukaryotic Transcription
Online Study Material on Eukaryotic and Prokaryotic Transcription
Study Material on Post Transcriptional Processing
Online Study material on Transcription
Online Study Material on Ribozyme
Link for Riobozyme, Catalytic RNA
Online Study Material on Translation I
Online Study Material on Protein Synthesis II
Assignment 2
Assignment on Transcription [Due Date: 01/03/2021]
Unit IV
Study Material on Bacterial Genetic Mapping: Transformation, Conjugation, Transduction
Online Study Material on Gene Mapping in Bacteria
Online Study Material : Review on Bacterial Genome Mapping
Study Material on Deletion Mapping
Online Study Material on Bacteriophage Deletion Mapping
Study Material on Bacteriophage Genome Mapping
Online study Material on Genetic transfer in Bacteria; and Bacteriophage
Online Study Material on Bacteripophage genome Mapping: Recombination analysis and Complementation
Online Study Material on P element
Study Material on Transposition
Online Study Material on Transposable Elements