BSc I Sem II
B.Sc. Semester 2 Microbiology
Microbial techniques
Name of the Teacher: Dr. B.V. Mohite
Zoom Link
Join Zoom Meeting
https://us04web.zoom.us/j/73124595081?pwd=WHNXbGpjRGo5VTZCRkxmUG92SjQ1UT09
Meeting ID: 731 2459 5081
Passcode: 8BGd0M
Unit I: Microscopy: Principle and Applications
Zoom Online Lectures
Day 1: Introduction to the syllabus (09/03/2021, 12.15 pm)
Day 2: Introduction and Parts of Microscope (15/03/2021, 12.15 pm)
Day 3: Principles of Microscopy (16/03/2021, 12.15 pm)
Day 4: Bright field Microscopy (22/03/2021, 12.15 pm)
Day 5: Dark Field Microscopy (23/03/2021,12.15 pm)
Day 6: Phase Contrast Microscopy (30/03/2021, 12.15 pm)
Day 7: Fluorescence Microscopy (05/04/2021, 12.15 pm)
Assignment 1 : Principles of Microscopy (Last Date of Submission : 10th April 2021)
Day 8: Confocal Microscopy (06/04/2021, 12.15 pm)
Day 9: Electron Microscopy (12/04/2021, 12.15 pm)
Day 10: Transmission Electron Microscopy Part 1 (19/04/2021, 12.15 pm)
Day 11: TEM Part 2 and Scanning Electron Microscopy (20/04/2021, 12.15 pm)
Assignment 2: Types of Microscopy (Last Date of Submission : 5th May 2021)
Unit Test I: Microscopy Techniques (2nd May 2021, 5.00 pm)
Unit I: Staining Techniques
Day 12: Dyes and stain, Concept and components, Types of stain (26/04/2021, 12.15 pm)
Day 13: Simple Staining (27/04/2021, 12.15 pm)
Day 14: Differential Staining: Gram Staining (03/05/2021, 12.15 pm)
Day 15: Differential Staining: Acid Fast Staining (04/05/2021, 12.15 pm)
Day 16: Endospore staining: Dorner's Method (10/05/2021, 12.15 pm)
Day 17: Endospore staining: Schaffer and Fulton's Method (10/05/2021, 12.15 pm)
Day 18: Capsule Staining (Positive staining method) (17/05/2021, 12.15 pm)
Day 19: Capsule staining (Negative staining method) (18/05/2021, 12.15 pm)
Day 20: Flagella structure and method of smear preparation for its staining (24/05/2021, 12.15 pm)
Day 21: Flagella staining by Leifson's method (25/05/2021, 12.15 pm)
Day 22: Metachromatic granule staining Alberts methiod (31/05/2021, 12.15 pm)
Day 23: Metachromatic granule staining Neissers method (01/06/2021, 12.15 pm)
Day 24: Revision of syllabus (07/06/2021, 12.15 pm)
Assignment 3: Staining Techniques (Last date of submission: 4th June 2021)
Unit Test II : Staining Techniques (8th June 2021, 5.00 pm)
Study Material on Introduction to Microscope, Parts and Types
Study Material on Principles of Microscopy
Study Material on Bright Field Microscopy
Study Material on Dark Field Microscopy
Study Material on Phase Contrast Microscopy
Study Material on Fluorescence Microscopy
Study Material on Confocal Microscopy
Online Study Material on Electron Microscopy
Study Material on Scanning Electron Microscopy
Study Material on Transmission Electron Microscopy
Unit II
Study Material on Dyes and Stain, chromophore, auxochrome and chromogen and Types of Stain
Study Material on Simple Staining
Study Material on Differential : Gram Staining
Study Material on Differential: Acid Fast Staining
Study Material on Endospore staining
Study Material on Capsule staining
Study Material on Flagella Staining
Study Material on Metachromatic granule staining
e books
Microbiology by Pelczar, McGraw-Hill Education
Basics of Microscopy by Martin Spitaler , Imperial College, London
Dr. Mrs. S.S. Khandare
Unit III: Microbial Reproduction and Growth
Study Material:
Growth ,Generation Time
drive.google.com/file/d/1YyD1o-FIANZ1imYlyzGIGI5FUCielptg/view?usp=sharing
Mathematical Expression for Growth
drive.google.com/file/d/1JjUS-Uw5lHuOeAcxqEK8I_tqO-uAOcta/view?usp=sharing
Growth Curve of Bacteria
drive.google.com/file/d/1KRZyRaEmAVrjoRNtZPevPy5bFqPMAzhm/view?usp=sharing
Continious Culture of Microorganisms
drive.google.com/file/d/1bSZsAy9QsEyvrvkb9s0F9S3JiEhqRneg/view?usp=sharing
Factors affecting Microbial growth
drive.google.com/file/d/1fZsDByvaALPnvbCPAVYlWMH5zcLLRtXb/view?usp=sharing
Unit-IV: Microbial Control.
Study Material:
Mr. M.G. Ingale
Unit V: Chemical Control Agents
Links for EBook:
Links for online Study Material:
Links for Video lectures:
Video lecture on Alcohol Part 1
Unit VI: Microbial interaction
Links for online Study Material:
Links for Video lectures: