B.Sc. Semester 2 Microbiology

Microbial techniques

Name of the Teacher: Dr. B.V. Mohite

Zoom Link

Join Zoom Meeting

https://us04web.zoom.us/j/73124595081?pwd=WHNXbGpjRGo5VTZCRkxmUG92SjQ1UT09

Meeting ID: 731 2459 5081

Passcode: 8BGd0M

Unit I: Microscopy: Principle and Applications

Zoom Online Lectures

Day 1: Introduction to the syllabus (09/03/2021, 12.15 pm)

Day 2: Introduction and Parts of Microscope (15/03/2021, 12.15 pm)

Day 3: Principles of Microscopy (16/03/2021, 12.15 pm)

Day 4: Bright field Microscopy (22/03/2021, 12.15 pm)

Day 5: Dark Field Microscopy (23/03/2021,12.15 pm)

Day 6: Phase Contrast Microscopy (30/03/2021, 12.15 pm)

Day 7: Fluorescence Microscopy (05/04/2021, 12.15 pm)

Assignment 1 : Principles of Microscopy (Last Date of Submission : 10th April 2021)

Day 8: Confocal Microscopy (06/04/2021, 12.15 pm)

Day 9: Electron Microscopy (12/04/2021, 12.15 pm)

Day 10: Transmission Electron Microscopy Part 1 (19/04/2021, 12.15 pm)

Day 11: TEM Part 2 and Scanning Electron Microscopy (20/04/2021, 12.15 pm)

Assignment 2: Types of Microscopy (Last Date of Submission : 5th May 2021)

Unit Test I: Microscopy Techniques (2nd May 2021, 5.00 pm)

Unit I: Staining Techniques

Day 12: Dyes and stain, Concept and components, Types of stain (26/04/2021, 12.15 pm)

Day 13: Simple Staining (27/04/2021, 12.15 pm)

Day 14: Differential Staining: Gram Staining (03/05/2021, 12.15 pm)

Day 15: Differential Staining: Acid Fast Staining (04/05/2021, 12.15 pm)

Day 16: Endospore staining: Dorner's Method (10/05/2021, 12.15 pm)

Day 17: Endospore staining: Schaffer and Fulton's Method (10/05/2021, 12.15 pm)

Day 18: Capsule Staining (Positive staining method) (17/05/2021, 12.15 pm)

Day 19: Capsule staining (Negative staining method) (18/05/2021, 12.15 pm)

Day 20: Flagella structure and method of smear preparation for its staining (24/05/2021, 12.15 pm)

Day 21: Flagella staining by Leifson's method (25/05/2021, 12.15 pm)

Day 22: Metachromatic granule staining Alberts methiod (31/05/2021, 12.15 pm)

Day 23: Metachromatic granule staining Neissers method (01/06/2021, 12.15 pm)

Day 24: Revision of syllabus (07/06/2021, 12.15 pm)

Assignment 3: Staining Techniques (Last date of submission: 4th June 2021)

Unit Test II : Staining Techniques (8th June 2021, 5.00 pm)

Study Material

Unit I

Study Material on Introduction to Microscope, Parts and Types

Study Material on Principles of Microscopy

Study Material on Bright Field Microscopy

Study Material on Dark Field Microscopy

Study Material on Phase Contrast Microscopy

Study Material on Fluorescence Microscopy

Study Material on Confocal Microscopy

Online Study Material on Electron Microscopy

Study Material on Scanning Electron Microscopy

Study Material on Transmission Electron Microscopy

Unit II

Study Material on Dyes and Stain, chromophore, auxochrome and chromogen and Types of Stain

Study Material on Simple Staining

Study Material on Differential : Gram Staining

Study Material on Differential: Acid Fast Staining

Study Material on Endospore staining

Study Material on Capsule staining

Study Material on Flagella Staining

Study Material on Metachromatic granule staining

e books

Microbiology by Pelczar, McGraw-Hill Education

Online Microscopy Notes

Basics of Microscopy by Martin Spitaler , Imperial College, London

Dr. Mrs. S.S. Khandare

Unit III: Microbial Reproduction and Growth

Study Material:

1. Growth ,Generation Time

drive.google.com/file/d/1YyD1o-FIANZ1imYlyzGIGI5FUCielptg/view?usp=sharing

2. Mathematical Expression for Growth

drive.google.com/file/d/1JjUS-Uw5lHuOeAcxqEK8I_tqO-uAOcta/view?usp=sharing

3. Growth Curve of Bacteria

drive.google.com/file/d/1KRZyRaEmAVrjoRNtZPevPy5bFqPMAzhm/view?usp=sharing

4. Continious Culture of Microorganisms

drive.google.com/file/d/1bSZsAy9QsEyvrvkb9s0F9S3JiEhqRneg/view?usp=sharing

5. Factors affecting Microbial growth

drive.google.com/file/d/1fZsDByvaALPnvbCPAVYlWMH5zcLLRtXb/view?usp=sharing

Unit-IV: Microbial Control.

Study Material:

1. General Terminologies: sterilization, disinfection, disinfectants, sanitizer, antisepsis, microbiostatic, microbiocidal, sanitizer, preservation.

2. Physical methods of control: Heat, moist heat sterilization

3. Dry heat sterilization

4. Physical methods of control: Low temperature

5. Filtration

6. Radiation

7. Osmotic pressure

Mr. M.G. Ingale

Unit V: Chemical Control Agents

Links for EBook:

1. Microbiology by pelczar

Links for online Study Material:

1. Characteristics of ideal disinfectant

2. Study material : Phenol

3. Study material : Alcohols

4. Study material : Heavy metals

5. Study material: Halogens

6. Study material: Surface active agents

7. Study material: Aldehydes

8. Study material: Gaseous sterilization

9. Study material: Chemotherapeutic agents

10. Study material: Mechanism of cell injury

11. Study material: Factors influencing antimicrobial activity

12. Study material: Phenol coefficient

Links for Video lectures:

1. Video lecture on Characteristics of ideal disinfectants

2. Video lecture on: Phenolic compounds part 1

3. Video lecture on phenolic compounds part 2

4. Video lecture on Alcohol Part 1

5. Video lecture on Alcohol Part 2

6. Video lecture: Heavy metals part 1

7. Video lecture: Heavy metals part 2

8. Video lecture on Halogens: Iodine

9. Video lecture: Halogen (Chlorine)

10. Video lecture on Surface active agents

11. Video lecture on Quaternary ammonium compounds

12. Video lecture: Gaseous sterilization

13. Video lecture: Chemotherapeutic agents part 1

14. Video lecture: Chemotherapeutic agents part 2

15. Mechanism of cell injury part 1

16. Mechanism of cell injury part 2

17. Factors influencing antibacterial activity

18. Phenol coefficient

Unit VI: Microbial interaction

Links for online Study Material:

1. Study material on Mutualism & Syntrophism

2. Study material on Commensalism & Synergism

3. Study material on Antagonism

4. Study material on parasitism & predation

5. Study material on Competetion

6. Study material on Protist-Protist Interaction: Bdellovibrio

7. Study material on Protist-Plant interaction: Root nodule bacteria

8. Protist-Animal interaction: Rumen bacteria

9. Study material on Insect midgut bacteria.

10. Study material on luminescent bacteria

Links for Video lectures:

1. Video lecture: Mutualism & Syntrophism part 1

2. Video lecture: Mutualism & Syntrophism part 2

3. Video lecture: Commensalism & synergism

4. Synergism continue..

5. Antagonism & predation part 1

6. Antagonism & predation part 2

7. Paracitism & competetion

8. Luminescent bacteria

9. Insect midgut bacteria

10. Protist-Animal interaction: Rumen bacteria

11. Protist-Protist Interaction: Bdellovibrio

SEM II Practical Course

• Effect of salt on bacterial growth

• Effect of radiation on bacterial growth

• Determination of Phenol coefficient

• Cultivation of anaerobic bacteria

• Antibiotic disc sensitivity test