Dr. Mrs. S.S. Khandare

Unit I:

Links for Video lecture:

1. Concept of gene: Split gene & overlapping gene

2. Gene Mutation Part -1

3. Gene mutation Part-2

4. Genetic Suppression part1

5. Genetic Suppression part2

Links for Study Material:

1. Definition, Basic concept & General types of recombination.

2. Transformation.

3. Conjugation

4. Transduction

5. Transposable genetic elements (Prokaryotic)

Unit II: Genetic code and protein synthesis

Links for Study Material:

1. Characteristics of genetic code

2. Deciphering of code

3. Wobble hypothesis

4. Colinearity of gene structure & its polypeptide products

5. Transcription (Prokaryotes)

6. Protein synthesis: Outline, process of translation (Prokaryotes)

Mr. M.G. Ingale

Unit III: Tools & Techniques of Genetic engineering

Links for Ebook:

1. Biotechnology by David Clark

Links for study material:

1. Study Material: Definition and Scope of Biotechnology & rDNA technology

2. Study material: Preparation of pure sample of DNA

3. Study material on analysis of DNA fragments size & Joining of DNA

4. Study material on vector & its types

5. Study material: Introduction of rDNA into host cell

6. Study material: Identification of transformed cells, selection of clones

7. Study material: Polymerase chain reaction & its application

8. Study material: DNA Fingerprinting

Links for video lecture

1. Video lecture on Definition & scope of biotechnology & rDNA technology Part 1

2. Video lecture on definition & scope of Biotechnology & rDNA technology part 2

3. Video lecture on preparation of pure DNA from bacteria part 1

4. Video lecture on preparation of pure DNA from bacteria part 2

5. Video lecture: Preparation of pure DNA from plant & preparation of plasmid DNA from bacteria

6. Video lecture: Preparation of pure DNA from bacteriophage

7. Video lecture: Enzymes used in DNA manipulation part 1

8. Video lecture: Enzymes used in DNA manipulation Part 2

9. Video lecture: Enzymes used in DNA manipulation Part 3

10. Video lecture: analysis of DNA fragment size & joining of DNA fragments part 1

11. Video lecture: analysis of DNA fragment size & joining of DNA fragments part 2

12. Video lecture: Vectors & their types part 1

13. Video lecture: Vectors & their types part 2

14. Video lecture: Vectors & their types part 3

15. Video lecture: Introduction of rDNA into host cell

16. Video lecture: identification of transformed cells, selection of clones – part 1

17. Video lecture: identification of transformed cells, selection of clones – part 2

18. Video lecture: identification of transformed cells, selection of clones – part 3

19. Video lecture: PCR & its application

20. Video lecture: DNA finger printing