M.Sc I Sem I
M.Sc. Microbiology Semester 1
Syllabus : M.Sc Microbiology Syllabus
Paper 1:
Microbial Metabolism (MM)
(Name of the Teacher: M. G. Ingale)
Mayur Ingale is inviting you to a scheduled Zoom meeting.
Topic: M.Sc Microbiology (Sem I) Lecture
Time: Feb 1, 2021 12:00 PM India
Join Zoom Meeting
https://us04web.zoom.us/j/79979727431?pwd=WHVrVitkU2JSMDNiTndraUZsZ2p1Zz09
Meeting ID: 799 7972 7431
Passcode: Ln22iv
Links for Ebook:
Links for Study material:
UNIT-I: - Bioenergetics and metabolism
Links for Study material:
UNIT-II: - Proteins and Nucleic acids
UNIT-III: - Photosynthesis
UNIT-IV:-Nitrogen and Sulphur metabolism and methanogenesis.
Links for Video lecture:
UNIT-III: - Photosynthesis
UNIT-IV:-Nitrogen and Sulphur metabolism and methanogenesis.
Paper : II
Enzymology and Techniques (ET)
Name of the Teacher : Dr. Bhavana V. Mohite
Zoom online lecture:
Join Zoom Meeting, Recurring Meeting
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Meeting ID: 78964849901
Passcode: TJbPa2
Day 1: 4th February 2021, 4.00 pm : Introduction to Syllabus
Unit I: Catalytic Mechanisms
Day 2: 5th February 2021, 2.00 pm :
Day 3: 6th February 2021, 12.30 pm :
Day 4: 8th February 2021, 3.00 pm :
Day 5: 9th February 2021, 3.30 pm :
Day 6: 10th February 2021, 12.00 pm :
Day 7: 11th February 2021, 3.00 pm :
Day 8: 12th February 2021, 3.00 pm :
Day 9: 13th February 2021, 12.00 pm :
Day 10: 15th February 2021, 12.00 pm :
Unit Test I : 15th March 2021, 4.00 Pm
Assignment II :Classification and Types of Enzyme (Last Date of Submission 22nd March 20201)
Unit II: Catalytic Mechanisms
Day 11: 16th February 2021, 3.00 pm :
Day 12: 17th February 2021, 12.00 pm :
Day 13: 18th February 2021, 3.00 pm :
Day 14: 20th February 2021, 12.30 pm :
Day 15: 23th February 2021, 12.00 pm :
Day 16: 24th February 2021, 3.00 pm :
Day 17: 25h February 2021, 3.00 pm :
Day 17: 26h February 2021, 3.00 pm :
Day 18: 27h February 2021, 9.00 am :
Day 19: 2 March 2021, 12.00 pm :
Assignment I : Types of Enzyme catalysis Mechanism (Last Date of Submission 8th March 20201)
Unit Test II : 25th March 2021, 4.00 Pm
Unit IV: Techniques
Day 20: 4 March 2021, 1.10 pm :
Day 21: 6 March 2021, 12.30 pm :
Day 22: 8 March 2021, 11.30 am :
Day 23 10 March 2021, 10.30 am :
Day 24: 12 March 2021, 12.00 pm :
Day 25: 15 March 2021, 10.30 am :
Day 26: 16 March 2021, 3.30 pm :
Day 27: 17 March 2021, 02.00 pm :
Day 28: 18 March 2021, 12.00 pm :
Day 29: 19 March 2021, 02.30 pm :
Day 30: 20 March 2021, 12.00 pm :
Day 31: 22 March 2021, 3.00 pm : Ion Exchange Chromatography for protein purification
Day 32: 23 March 2021, 3.00 pm : Gel Electrophoresis for protein Purification
Day 33: 24 March 2021, 11.30 am Isoelectrofocussing for protein purification
Day 35: 31st March 2021, 3.00 pm Enzyme Assays
Day 36: 1st April 2021, 3.00 pm Direct Enzyme Assays
Day 37: 7th April 2021, 1.00 pm Spectrophotometric and flurometric assays
Day 38: 8th April 2021, 11.00 am Calorimetric, Polarometric and discontinuous Assay
Day 39: 15th April 2021, 1.00 pm General Concepts of Biosensor
Day 40: 21st April 2021, 1.00 pm Glucose biosensor (Part 1)
Day 41: 28th April 2021, 12.00 pm Applications of biosensor and challenges
Day 42: 29th April 2021, 3.00 pm Industrial applications of enzymes
Day 43: 5th May 2021, 11.00am Introduction, objectives of Protein engineering
Day 44: 6th May 2021, 12.00 pm Steps of Protein engineering
Assignment III: Unit 4 Techniques (Last date of submission 13th May 2021)
Unit Test III: 12th May 2021 12.00 pm
Day 45: 13th April 2021, 12.00 pm Protein engineering methods
Day 46: 19th April 2021, 12.00 pm Protein engineering methods (Part 2)
Day 47: 20th April 2021, 12.00 pm Discussion on Test
Unit Test IV: 27th May 2021, 3.00 pm
Unit III: Enzyme Kinetics and Regulation
Day 48: 2nd June 2021, 3.00 pm Michaelis Menten Equation
Day 49: 3rd June 2021, 400 pm Michaelis Menten equation and its transformation in LB plot
Day 50: 4th June 2021, Enzyme Inhibition kinetics and its Types
Day 51: 4th June 2021, Enzyme Inhibition kinetics and its Types 2
Day 52: 7th June 2021, Bi substrate kinetics
Day 53: 8th June 2021, Multisubstrate kinetics
Day 54: 10th June 2021 Allosteric regulation
Day 55: 9th June 2021, Feedback Inhibition and Allosteric regulation
Day 56: 11th June 2021 Allosteric kinetics Covalent Modification
Study Material
Unit I: Catalytic Mechanisms
Study Material on Enzyme Introduction, Catalytic Activity, active site models
Study Material on Classification of Enzymes
Online Study Material on Enzyme terminologies, nomenclature and classification
Study Material on memberane bound and marker enzymes
Study Material on Multienzyme Complex
Study Material on Marker Enzymes
Study Material on Enzyme Types and catalysis
Unit II: Catalytic Mechanisms
Online Study Material on Types of Mechanism of Catalysis
Online study Material on Enzyme catalysis Mechanism
Study Material on Serine Protease
Online Study Material on Enzyme Mechanism Serine Protease
Online Study material on Serine ProteaseStudy Material on Serine Protease 1
Online Study Material on Mechanism of Lysozyme
Online Study Material on Lysozyme
Unit IV: Techniques
Study Material on Purification of Proteins
Study Material on General Strategy for Purification of Enzymes
Study Material on enzyme production and Purification m
Study Material on Purification of Enzymes
Study Material on Isolation, Purification and characterization of enzymes
Study Material on Enzyme assays
Study Material on Enzyme assays 1
Study Material on Enzyme assays 2
Study Material on Protein Ligand binding studies
Study Material on Oxygen binding by Myoglobin and Haemoglobin
Study Material on MWC and Sequential model
Study Material on ligand Binding
Online study material on Glucose biosensor
Online study material on Biosensors
Study Material on Biosensors 1
Study Material on Industrial applications of enzyme
Study Material on Industrial applications of enzyme 2
Online study Material on Protein Engineering
Online study material on Protein Engineering 2
Unit III: Enzyme Kinetics and Regulation
Study Material on MM equation derivation and plot, its Transformation to other plots
Study Material on Derivation of Michaelis Menten equation
Study Material on Enzyme Inhibition Types
Study Material on Enzyme Inhibition kinetics
Study Material on Bisubstrate kinetics
Study Material on Regulatory Enzyme
Study Material Allosteric regulation , feedback inhibition, Covalent Modification
Study Material on MWC and Sequential model
Study Material on Kinetics of Allosteric enzyme
e book
Molecular Cell Biology. 4th edition by Lodish H, Berk A, Zipursky SL, et al.
Enzymes: Biochemistry, Biotechnology, Clinical Chemistry By T Palmer and P L Bonner
PRACTICAL-I
PG- MB (08) - S1-P1
LABORATORY EXERCISE 1
Demonstration Videos
Determination of kinetic constant of amylase:-Amylase activity, Vmax, Km.
https://www.youtube.com/watch?v=8NzyAXnNFGo
Effect of pH and temperature on amylase activity.
https://www.youtube.com/watch?v=JUQExVhugFI
https://www.youtube.com/watch?v=CWt7Lg-QhjM
UV absorption of proteins, DNA and RNA.
https://www.youtube.com/watch?v=VGXVbpyWsmY
Estimation of protein by Lowry’smethod.
https://www.youtube.com/watch?v=QN2AfWJjW0s
Effect of inhibitors on amylase activity.
https://www.youtube.com/watch?v=U2It7pEZLo8
Study Material
PRACTICAL-II
PG- MB (08) - S1-P2
LABORATORY EXERCISE 2
Video Links:
Separation of DNA by agarose gel electrophoresis
https://www.youtube.com/watch?v=vq759wKCCUQ
Separation of amino acids by paper chromatography
https://www.youtube.com/watch?v=tDaKxskUwA0
https://www.youtube.com/watch?v=8wmQ_xWqZbo
Separation of serum proteins by paper electrophoresis
https://www.youtube.com/watch?v=FKxQxt2vAK4
https://www.youtube.com/watch?v=jt1a8AUeJcg
Thin layer chromatography.
https://www.youtube.com/watch?v=qdmKGskCyh8
https://www.youtube.com/watch?v=lj5OWzhZSac
SDS-Page of proteins
https://www.youtube.com/watch?v=i_6y6Z5UvwE
https://www.youtube.com/watch?v=iqTHx9aUVcY
Performance of Gel filtration chromatography
https://www.youtube.com/watch?v=qrUaZWUM9uw
Demonstration of blotting technique
https://www.youtube.com/watch?v=VgAuZ6dBOfs
https://www.youtube.com/watch?v=3I9wzwj0b_A
Ion exchange chromatography
https://www.youtube.com/watch?v=lp40a7mtc4E
https://www.youtube.com/watch?v=i4U4ndf2ayg
· Separation of Sub cellular organelles and isolation of Marker enzymes.
https://www.jove.com/v/52076/isolation-functional-analysis-mitochondria-from-cultured-cells-mouse
Study Material: