조한상

약력

    Dec. 2023 – present                                  NeuroLynx Ltd. Republic of Korea                                                                         CEO

Sep. 2019 – present                                   Dep. Biophysics, Sungkyunkwan University                                                       Associate Professor

July 2017 – June 2018                               National University of Singapore                                                                           Visiting Research Scientist

Aug. 2014 – June 2020                             Mechanical Engineering and Engineering Science,                                       Assistant Professor

                                                                       University of North Carolina at Charlotte,                                                          Postdoctoral Research Fellow

Sep. 2010 – Aug. 2014                               Harvard Medical School, Massachusetts General Hospital, 

                                                                       and Shriners Hospital

Jan. 2003 – Aug. 2005                              Korea Institute of Science and Technology                                                       Research Scientist

Mar. 1999 – Jan. 2003                               Biomedlab Co. Republic of Korea                                                                         Team Manager

강의제목

     3D Neurovascular-Glial Micro-Pathophysiological System

강의내용

The choroid plexus, where the blood-cerebrospinal fluid barrier (BCB) is located, plays a crucial role in regulating the entry of immune cells and molecules into the brain as well as removing neurotoxic substances. However, the study of BCB pathologies is challenging in the absence of simple and relevant models. Here, we present a blood-choroid plexus microphysiological system that integrates the choroid plexus, monocytes, macrophages, and blood-brain barrier (BBB) tissue chips to simulate the effects of neurotoxic substances in the BCB region. First, we validated the functional properties of BCB models by showing the restriction of monocytes, the leakage of FICT-dextran dye (M.W. 70 kDa) from blood-to-brain, the localization of ZO-1 and VE-cad in both brain endothelium and choroid plexus epithelium, and cuiboidal structures in the choroid plexus epithelium. Second, we confirmed the dysfunction of the blood-barrier side in the blood-to-CSF entry pathway by demonstrating that direct exposure of the BBB to LPS (10 ng/ml) and PFF (100 ng/ml) results in an increase in blood-barrier permeability, a reduction of ZO-1 and VE-Cad in endothelium, and the deposition of monocytes and macrophages into the CSF region, causing inflammation. Third, we reported that deposition of oligomer amyloid beta (100 ng/ml) in the CSF region induced choroid plexus epithelium dysfunction, as evidenced by a decrease in ZO-1 and TTR expression as well as the infusion of monocytes and macrophages into the CSF. We anticipate that our human blood-choroid plexus model will facilitate the elucidation of blood-cerebrospinal fluid barrier (BCB) pathophysiology and contribute to the development of therapeutic agents for neurodegenerative disease.

관련 논문

1 M. Tran, H.W. Jeong, M. An, C. Been, A. Jamsranjav, S.M. Kwak, L.P. Lee, H. Cho, “A 3D Gut-Brain-Vascular Platform for Bidirectional Crosstalk         in Gut-Neuropathogenesis,” Nature Communications 2026, accepted

2 Y.J. Kang, Y.N. Diep, M. Tran, V.T.A Tran, G. Ambrin, H. Ngo, H. Cho*, “Three-dimensional Human Neural Culture on a Chip Recapitulating                Neuroinflammation and Neurodegeneration,” Nature Protocols 2023, 18;9: 2838 (cover imaged)

3 H. Cho, J.H. Seo, K.H.K. Wong, Y. Terasaki, J. Park, K. Bong, K. Arai, E.H. Lo, D. Irimia, “Three-Dimensional Blood-Brain Barrier Model for in 

        vitro Studies of Neurovascular Pathology,” Scientific Reports 2015, 5:15222