Publication

[260] "Enhanced Cell Growth, Production, and mAb Quality in CHO-K1 Cells by Supplementing Polyamines in the Media", D. E. Kang, Y. B. An, Y. Kim, S. Ahn, Y. J. Kim, J. S. Lim, S. H. Ryu, H. Kim, J. Yoo, W.-K. You, D.-Y. Lee, J. Park, M. Hong, G. M. Lee, J. Y. Baik, J. K. Hong, Appl. Microbiol. Biotechnol. 10:2855 (2023).

[259] ”Enhancing CHO Cell Productivity Through a Dual Selection System Using Aspg and Gs in Glutamine Free Medium”, T. K. Ha, A. Òdena, K. J. la C. Karottki, C. L. Kim, H. Hefzi, G. M. Lee, H. F. Kildegaard, L. K. Nielsen, L. M. Grav, N. E. Lewis, Biotechnol. Bioeng. (2023) 

[258] “Improving Bone Morphogenetic Protein (BMP) Production in CHO Cells through Understanding of BMP Synthesis, Signaling and Endocytosis”, K. Kim, M. G. Kim, G. M. Lee, Biotechnol. Adv. (2023). 

[257] “Development of an in Vitro Screening System for Synthetic Signal Peptide in Mammalian Cell-Based Protein Production", J. H. Park, H. M. Lee, E. J. Jin, E. J Lee, Y. J. Kang, S. Kim, S. S. Yoo, G. M. Lee, Y. G. Kim, Appl. Microbiol. Biotechnol. (2022).

[256] “Recombinase-Mediated Cassette Exchange-Based Screening of a CRISPR/Cas9 Library for Enhanced Recombinant Protein Production in Human Embryonic Kidney Cells: Improving Resistance to Hyperosmotic Stress”, S. Shin, S. H. Kim, J. H. Park, J. S. Lee, G. M. Lee, Metabolic Eng. (2022).

[255] “Small Molecule Epigenetic Modulators for Enhancing Recombinant Protein Expression in CHO Cell Cultures”,  D. Kim, C. Yoon, and G. M. Lee, Biotechnol. Bioeng. (2022). 

[254] “Improving  the Secretory Capacity of CHO Producer Cells: the Effect of Controlled Blimp1 Expression, a Master Transcription Factor for Plasma Cells”, S. H. Kim, M. Baek, S. Park, S. Shin, J. S. Lee, G. M. Lee, Metabolic Eng. (2022).

[253] “Factors Affecting the Quality of Therapeutic Proteins in Recombinant Chinese Hamster Ovary Cell Culture”, T. K. Ha, D. Kim, C. L. Kim, L. M. Grav, and G. M. Lee, Biotechnol. Adv. (2021). 

[252] “A Chinese Hamster Transcription Start Site Atlas That Enables Targeted Editing of CHO Cells” I. Shamie, S. Duttke, K. Karottki, C. Han, A. Hansen, H. Hefzi, K. Xiong, S. Li, S. Roth, J. Tao, G. M. Lee, Glass, Christopher; H. Kildegaard, C. Benner, and N.E. Lewis, NAR Genom. Bioinform. 3:11 (2021).  

[251] “An Optimized Genome-Wide, Virus-Free CRISPR Screen for Mammalian Cells", K. Xiong, K. J. la Cour Karottki, H. Hefzi, Li S, L. M. Grav, S. Li, P. Spahn, J. S. Lee, G. M. Lee, H. F. Kildegaard, N. E. Lewis, and L. E. Pedersen, Cell Rep. Methods (2021). 

[250] ”Blockage of Undesirable Endocytosis of Recombinant Human Growth/Differentiation Factor-5 in Chinese Hamster Ovary Cell Cultures Requires Heparin Analogs with Specific Chain Lengths”, M. G. Kim and G. M. Lee, Biotechnol. J. (2021).

[249] “Streamlined Human Cell-Based Recombinase-Mediated Cassette Exchange Platform Enables Multigene Expression for the Production of Therapeutic Proteins”, S. Shin, S. H. Kim, J. S. Lee, and G. M. Lee, ACS Synth. Biol. (2021). 

[248] “A Metabolic CRISPR-Cas9 Screen in Chinese Hamster Ovary Cells Identifies Glutamine-Sensitive Genes”,   K. J. la Cour Karottki, H. Hefzi, S. Li, L. E. Pedersen, P. N. Spahn, C. Joshi, D. Ruckerbauer, J. A. H. Bort, A. Thomas, J. S. Lee, N. Borth, G. M. Lee, H. F. Kildegaard, N. E. Lewis, Metabolic Eng. 66:114 (2021).

[247] "Selective Endocytosis Recombinant Human BMPs Through Cell Surface Heparan Sulfate Proteoglycans in CHO Cells: BMP-2 and BMP-7", M. G. Kim, C. L. Kim, Y. S. Kim, J. W. Jang, and G. M. Lee, Sci. Rep. 11:3378 (2021).  

[246]  “Comprehensive Characterization of Dihydrofolate Reductase-Mediated Gene Amplification for the Establishment of Recombinant Human Embryonic Kidney 293 Cells Producing Monoclonal Antibodies”, S. Y. Lee, M. Baek, and G. M. Lee, Biotechnol. J. (2021). 

[245] "Amplification of EBNA-1 through a single-plasmid vector-based gene amplification system in HEK293 cells as an efficient transient gene expression system", S. H. Park, J. H. Park, J. H. Lee, H. M. Lee, Y. J. Kang, E. J. Lee, S. Shin, G. M. Lee and Y. G. Kim, Appl. Microbiol. Biotechnol. (2020).

[244] “Multi-Copy Targeted Integration for Accelerated Development of High-Producing CHO Cells”, D. Sergeeva, L. K. Nielsen, G. M. Lee, and L. M. Grav, ACS Synth. Biol. (2020). 

[243] “Forskolin Increases cAMP Levels and Enhances Recombinant Antibody Production in CHO Cell Cultures”, C. Yoon, D. Kim, J. H. Lim, and G. M. Lee, Biotechnol. J. (2020). 

[242] “Comprehensive Analysis of Genomic Safe Harbors as a Target Site for Stable Expression of Heterologous Gene in HEK293 Cells”, S. Shin, S. H. Kim, S. W. Shin, L. M. Grav, L. E. Pedersen, J. S. Lee, and G. M. Lee, ACS Synth. Biol. (2020). 

[241] “Multiplex Secretome Engineering Enhances Recombinant Protein Production and Purity”, S. Kol, D. Ley, T. Wulff, M. Decker, J. Arnsdorf, S. Schoffelen, A. H. Hansen, T. L. Jensen, J. M. Gutierrez, A. W. T. Chiang, H. O. Masson, B. O. Palsson, B. G. Voldborg, L. E. Pedersen, H. F. Kildegaard, G. M. Lee, and N. E. Lewis, Nat Commun. 11:1908. (2020).

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