Embedded Files
[281] “Genome-Wide CRISPR Screening Identifies Genes in Recombinant Human Embryonic Kidney 293 Cells for Increased Ammonia Resistance”, S. Y. Lee, H. S. Kim, Y. G. Kim, S. Shin, S. Kweon , J. J. Lee, G. M. Lee, Metabolic Eng. (2026).
[280]“Product-Stabilized Filamentation by Human Glutamine Synthetase Allosterically Tunes Metabolic Activity”, E. Greene, R. Muniz, H.Yamamura, S. Hoff, P. Bajaj, D. J. Lee, E. M.Thompson, A. Arada, G. M. Lee, M. Bonomi, J. M. Kollman, J. S. Fraser, eLife 14:RP108336 (2025).
[279] “Recombinase-Mediated Cassette Exchange-Based CRISPR Activation Screening Identifies Hyperosmotic Stress-Resistant Genes in Chinese Hamster Ovary Cells”, M. Baek, S. Kweon, Y. Kim, N. E. Lewis, J. S. Lee, G. M. Lee, ACS Synth. Biol. (2025).
[278] “Improving Recombinant Antibody Production Using FcBAR: An In Situ Approach to Detect and Amplify Protein-Protein Interactions”, M. Y. M. Wu, F. Rocamora, M. Samoudi, C. M. Robinson, C.-C. Kuo, N. Pristovšek, L. M. Grav, H. F. Kildegaard, G. M. Lee, A. R. Campos, N. E. Lewis, Metabolic Eng. (2025).
[277] “Multiplex Genome Editing Eliminates the Warburg Effect without Impacting Growth Rate in Mammalian Cells”, H. Hefzi, I. Martinez-Monge, I. M. de Mas, N. L. Cowie, A. G. Toledo, S. M. Noh, K. J. la Cour Karottki, M. Decker, J. Arnsdorf, J. M. Camacho-Zaragoza, S. Kol, S. Schoffelen, N. Pristovšek., A. H. Hansen, A. A. Miguez, S. P. Bjorn, K. K. Brøndum, E. M. Javidi, K. L. Jensen, L. Stangl, E. Kreidl, T. B. Kallehauge, D. Ley, P. Ménard, H. M. Petersen, Z. Sukhova, A. Bauer, E. Casanova, N. Barron, J. Malmström, L. K. Nielsen, G. M. Lee, H. F. Kildegaard, B. G. Voldborg, N. E. Lewis., Nat. Metab. (2025).
[276] “Genome-Scale CRISPR Screenings for Insights into Gene Essentiality and Functionality in CHO Cells”, S. W. Shin, S. H. Kim, A. Gasselin, G. M. Lee, J. S. Lee”, Scientific Data (2025).
[275] “Knockout of Proapoptotic BAX and BAK1 Genes in HEK293T Cells Enhances Adeno-Associated Virus (AAV) Production Regardless of AAV Serotype”, S. Park, S. Shin, G. Han, G. M. Lee, Biotechnol. J. (2025).
[274] “Unraveling Productivity-Enhancing Genes in Chinese Hamster Ovary Cells via CRISPR Activation Screening Using Recombinase-Mediated Cassette Exchange System”, M. Baek, C. L. Kim, S. H. Kim, K. J. la Cour Karottki, H. Hefzi, L. M. Grav, L. E. Pedersen, N. E. Lewis, J. S. Lee, G. M. Lee, Metabolic Eng. (2025).
[273] "Development, Physico-Chemical Characterization, and in Vivo Stability of a Novel Aglycosylated Monoclonal Antibody Targeting FAM19A5”, W. K. Kim, J. Y. Seong, G. M. Lee, Biotechnol. Bioproc. Eng. (2024).
[272]“SiMPl-GS: Advancing Cell Line Development via Synthetic Selection Marker for Next-Generation Biopharmaceutical Production”, C. Yoon, E. J. Lee, D. Kim, S. Joung, Y. Kim, H. Jung, Y. G. Kim, G. M. Lee, Adv. Sci. (2024).
[271]“Sialyllactose Supplementation Enhances Sialylation of Fc-Fusion Glycoprotein in Recombinant Chinese Hamster Ovary Cell Culture”, H. M. Lee, T. H. Kim, J. H. Park, N. Y. Heo, H. S. Kim, D. E. Kim; M. K. Kim, G. M. Lee, J. You, Y. G. Kim, J. Biotechnol. (2024).
[270]“Enhancing the Production of Adeno-Associated Virus (AAV)2 and AAV9 with High Full Capsid Ratio in HEK293 Cells through Design-of-Experiment Optimization of Triple Plasmid Ratio”, S. Park, S. Shin, H. Lee, J. H. Jang, G. M. Lee, Biotechnol. J. (2024).
[269]"Effects of Autophagy-Inhibiting Chemicals on Sialylation of Fc-Fusion Glycoprotein in Recombinant Chinese Hamster Ovary Cell Culture", H. M. Lee, J. H. Park, T. H. Kim, H. S. Kim, D. E. Kim; M. K. Lee, J. You, G. M. Lee, Y. G. Kim, Appl. Microbiol. Biotechnol. (2024).
[268] “Extraction of an Outer Membrane Protein Pertactin from Bordetella pertussis with Urea for the Production of Acellular Pertussis Vaccine”, J. H. Moon, J. K. Park, B. Y. Park, H. J. Jeon, G. S. Choi, G. M. Lee, Biotechnol. Bioproc. Eng. (2024).
[267]“Genome-Wide CRISPR/Cas9 Screening Unveils a Novel Target ATF7IP-SETDB1 Complex for Enhancing Difficult-to-Express Protein Production”, S. H. Kim, J. Park, S. Shin, S. Shin, D. Chun, Y. G. Kim, J. Yoo, W. K. You, J. S. Lee, G. M. Lee, ACS Synth. Biol. (2024).
[266]“Genome-Wide CRISPR/Cas9 Knockout Screening to Mitigate Cell Growth Inhibition Induced by Histone Deacetylase Inhibitors in Recombinant CHO Cells”, D. Kim, S. H. Kim, C. Yoon, G. M. Lee, Biotechnol. Bioeng. (2023).
[265] “In-Vitro Induction of In-Vivo-Relevant Stellate Astrocytes in 3D Brain-Derived, Decellularized Extracellular Matrices”, S. Han, J. Kim, S. H. Kim, W. Youn, J. Kim, G. Y. Ji, S. Yang, J. Park, G. M. Lee, Y. Kim, I. S. Choi, Acta Biomater. (2023).
[264] “Enhancing the Production of Recombinant Human TGF-β1 Through an Understanding of TGF-β1 Synthesis, Signaling, and Endocytosis in CHO Cells” K. Kim, Y. S. Kim, J. W. Jang, G. M. Lee, Biotechnol. J. (2023).
[263] “Identification of Hyperosmotic Stress-Responsive Genes in Chinese Hamster Ovary Cells via Genome-Wide Virus-Free CRISPR/Cas9 Screening”, S. H. Kim, S. Shin, M. Baek, K. Xiong, K. J. la C. Karottki, H. Hefzi, L. M. Grav, L. E. Pedersen, H. F. Kildegaard, N. E. Lewis, J. S. Lee, G. M. Lee, Metabolic Eng. (2023).
[262] “Mitigating Transcriptional Bottleneck Using a Constitutively Active Transcription Factor, VP16-CREB, in Mammalian Cells”, C. Yoon, K. E. Baek, D. Kim, G. M. Lee, Metabolic Eng. (2023).
[261] "Streamlined In Vitro Screening System of Synthetic Signal Peptides in Chinese Hamster Ovary Cells for Therapeutic Protein Production", J. H. Park, N. Y. Heo, H. M. Lee, E. J. Lee, S. Park, G. M. Lee, Y. G. Kim, J. Biotechnol. (2023).
[260] "Enhanced Cell Growth, Production, and mAb Quality in CHO-K1 Cells by Supplementing Polyamines in the Media", D. E. Kang, Y. B. An, Y. Kim, S. Ahn, Y. J. Kim, J. S. Lim, S. H. Ryu, H. Kim, J. Yoo, W.-K. You, D.-Y. Lee, J. Park, M. Hong, G. M. Lee, J. Y. Baik, J. K. Hong, Appl. Microbiol. Biotechnol. 10:2855 (2023).
[259] ”Enhancing CHO Cell Productivity Through a Dual Selection System Using Aspg and Gs in Glutamine Free Medium”, T. K. Ha, A. Òdena, K. J. la C. Karottki, C. L. Kim, H. Hefzi, G. M. Lee, H. F. Kildegaard, L. K. Nielsen, L. M. Grav, N. E. Lewis, Biotechnol. Bioeng. (2023)
[258] “Improving Bone Morphogenetic Protein (BMP) Production in CHO Cells through Understanding of BMP Synthesis, Signaling and Endocytosis”, K. Kim, M. G. Kim, G. M. Lee, Biotechnol. Adv. (2023).
[257] “Development of an in Vitro Screening System for Synthetic Signal Peptide in Mammalian Cell-Based Protein Production", J. H. Park, H. M. Lee, E. J. Jin, E. J Lee, Y. J. Kang, S. Kim, S. S. Yoo, G. M. Lee, Y. G. Kim, Appl. Microbiol. Biotechnol. (2022).
[256] “Recombinase-Mediated Cassette Exchange-Based Screening of a CRISPR/Cas9 Library for Enhanced Recombinant Protein Production in Human Embryonic Kidney Cells: Improving Resistance to Hyperosmotic Stress”, S. Shin, S. H. Kim, J. H. Park, J. S. Lee, G. M. Lee, Metabolic Eng. (2022).
[255] “Small Molecule Epigenetic Modulators for Enhancing Recombinant Protein Expression in CHO Cell Cultures”, D. Kim, C. Yoon, and G. M. Lee, Biotechnol. Bioeng. (2022).
[254] “Improving the Secretory Capacity of CHO Producer Cells: the Effect of Controlled Blimp1 Expression, a Master Transcription Factor for Plasma Cells”, S. H. Kim, M. Baek, S. Park, S. Shin, J. S. Lee, G. M. Lee, Metabolic Eng. (2022).
[253] “Factors Affecting the Quality of Therapeutic Proteins in Recombinant Chinese Hamster Ovary Cell Culture”, T. K. Ha, D. Kim, C. L. Kim, L. M. Grav, and G. M. Lee, Biotechnol. Adv. (2021).
[252] “A Chinese Hamster Transcription Start Site Atlas That Enables Targeted Editing of CHO Cells” I. Shamie, S. Duttke, K. Karottki, C. Han, A. Hansen, H. Hefzi, K. Xiong, S. Li, S. Roth, J. Tao, G. M. Lee, Glass, Christopher; H. Kildegaard, C. Benner, and N.E. Lewis, NAR Genom. Bioinform. 3:11 (2021).
[251] “An Optimized Genome-Wide, Virus-Free CRISPR Screen for Mammalian Cells", K. Xiong, K. J. la Cour Karottki, H. Hefzi, Li S, L. M. Grav, S. Li, P. Spahn, J. S. Lee, G. M. Lee, H. F. Kildegaard, N. E. Lewis, and L. E. Pedersen, Cell Rep. Methods (2021).
[250] ”Blockage of Undesirable Endocytosis of Recombinant Human Growth/Differentiation Factor-5 in Chinese Hamster Ovary Cell Cultures Requires Heparin Analogs with Specific Chain Lengths”, M. G. Kim and G. M. Lee, Biotechnol. J. (2021).
[249] “Streamlined Human Cell-Based Recombinase-Mediated Cassette Exchange Platform Enables Multigene Expression for the Production of Therapeutic Proteins”, S. Shin, S. H. Kim, J. S. Lee, and G. M. Lee, ACS Synth. Biol. (2021).
[248] “A Metabolic CRISPR-Cas9 Screen in Chinese Hamster Ovary Cells Identifies Glutamine-Sensitive Genes”, K. J. la Cour Karottki, H. Hefzi, S. Li, L. E. Pedersen, P. N. Spahn, C. Joshi, D. Ruckerbauer, J. A. H. Bort, A. Thomas, J. S. Lee, N. Borth, G. M. Lee, H. F. Kildegaard, N. E. Lewis, Metabolic Eng. 66:114 (2021).
[247] "Selective Endocytosis Recombinant Human BMPs Through Cell Surface Heparan Sulfate Proteoglycans in CHO Cells: BMP-2 and BMP-7", M. G. Kim, C. L. Kim, Y. S. Kim, J. W. Jang, and G. M. Lee, Sci. Rep. 11:3378 (2021).
[246] “Comprehensive Characterization of Dihydrofolate Reductase-Mediated Gene Amplification for the Establishment of Recombinant Human Embryonic Kidney 293 Cells Producing Monoclonal Antibodies”, S. Y. Lee, M. Baek, and G. M. Lee, Biotechnol. J. (2021).
[245] "Amplification of EBNA-1 through a single-plasmid vector-based gene amplification system in HEK293 cells as an efficient transient gene expression system", S. H. Park, J. H. Park, J. H. Lee, H. M. Lee, Y. J. Kang, E. J. Lee, S. Shin, G. M. Lee and Y. G. Kim, Appl. Microbiol. Biotechnol. (2020).
[244] “Multi-Copy Targeted Integration for Accelerated Development of High-Producing CHO Cells”, D. Sergeeva, L. K. Nielsen, G. M. Lee, and L. M. Grav, ACS Synth. Biol. (2020).
[243] “Forskolin Increases cAMP Levels and Enhances Recombinant Antibody Production in CHO Cell Cultures”, C. Yoon, D. Kim, J. H. Lim, and G. M. Lee, Biotechnol. J. (2020).
[242] “Comprehensive Analysis of Genomic Safe Harbors as a Target Site for Stable Expression of Heterologous Gene in HEK293 Cells”, S. Shin, S. H. Kim, S. W. Shin, L. M. Grav, L. E. Pedersen, J. S. Lee, and G. M. Lee, ACS Synth. Biol. (2020).
[241] “Multiplex Secretome Engineering Enhances Recombinant Protein Production and Purity”, S. Kol, D. Ley, T. Wulff, M. Decker, J. Arnsdorf, S. Schoffelen, A. H. Hansen, T. L. Jensen, J. M. Gutierrez, A. W. T. Chiang, H. O. Masson, B. O. Palsson, B. G. Voldborg, L. E. Pedersen, H. F. Kildegaard, G. M. Lee, and N. E. Lewis, Nat Commun. 11:1908. (2020).
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