Publication

[240] “Knockout of Sialidase and Pro-apoptotic Genes in Chinese Hamster Ovary Cells Enables the Production of Recombinant Human Erythropoietin in Fed-Batch Cultures”, T. K. Ha, A. H. Hansen, H. F. Kildegaard, and G. M. Lee, Metabolic Eng. 57:182 (2020).

[239] "Genome-Scale Reconstructions of the Mammalian Secretory Pathway Predict Metabolic Costs and Limitations of Protein Secretion", J. M. Gutierrez, A. Feizi, S. Li, T. B. Kallehauge, H. Hefzi, L. M. Grav, D. Ley, D. B. Hizal, M. J. Betenbaugh, B. Voldborg, H. F. Kildegaard, G. M. Lee, B. O. Palsson, J. Nielsen, N. E. Lewis, Nat. Commun. 11:68 (2020).

[238] “Awakening Dormant Glycosyltransferases in CHO Cells with CRISPRa”, K. J. la Cour Karottki, H. Hefzi, K. Xiong, I. Shamie, A. H. Hansen, S. Li, L. E. Pedersen, S. Li, J. S. Lee, G. M. Lee, H. F. Kildegaard, and N. E. Lewis, Biotechnol. Bioeng. (2019).

[237] “Bip Inducer X: an ER Stress Inhibitor for Enhancing Recombinant Antibody Production in CHO Cell Culture”, T. K. Ha, A. H. Hansen, H. F. Kildegaard, and G. M. Lee, Biotechnol. J. (2019). 

[236] “Mitigating Clonal Variation in Recombinant Mammalian Cell Lines”, J. S. Lee, H. F. Kildegaard, N. E. Lewis, G. M. Lee, Trends Biotechnol. (2019).

[235] “Reduced Apoptosis in Chinese Hamster Ovary Cells via Optimized CRISPR Interference”, K. Xiong, K. F. Marquart, K. J. la C. Karottki, S. Li, I. Shamie, J. S. Lee, N. C. Yeo, A. Chavez, G. M. Lee, N. E. Lewis, H. F. Kildegaard, Biotechnol. Bioeng. (2019). 

[234] “Systematic Evaluation of Site-Specific Recombinant Gene Expression for Programmable Mammalian Cell Engineering”, N. Pristovšek, S. Nallapareddy, L. M. Grav, H. G. Hansen, H. Hefzi, N. E. Lewis, P. Rugbjerg, G. M. Lee, M. R. Andersen, H. F. Kildegaard, ACS Synth. Biol. (2019). 

[233] “Analysis of Intracellular Golgi pH in CHO Cells Under Culture Process Conditions Using Ratiometric pH-Sensitive Fluorescent Proteins”, J. H. Lee, J. Kim, J. H. Park, W. D. Heo, G. M. Lee, Biotechnol. Bioeng. (2019).

[232] “Glyco-Engineered CHO Cell Lines Producing Alpha-1-Antitrypsin and C1 Esterase Inhibitor with Fully Humanized N-Glycosylation Profiles”, T. Amann, A. H. Hansen, S. Kol, H. G. Hansen, J. Arnsdorf, S. Nallapareddy, B. Voldborg, G. M. Lee, M. R. Andersen, H. F. Kildegaard, Metabolic Eng. (2019). 

[231] “Improving the Production of Recombinant Bone Morphogenetic Protein-4 (BMP-4) by Removal of Autoregulatory Feedback Loop Using BMP Receptor-Knockout CHO Cell Lines”, C. L. Kim and G. M. Lee, Metabolic Eng. (2019).

[230] “Revealing Key Determinants of Clonal Variation in Transgene Expression in Recombinant CHO Cells Using Targeted Genome Editing”, J. S. Lee, J. H. Park, T. K. Ha, M. Samoudi, N. E. Lewis, B. O. Palsson, H. F. Kildegaard, G. M. Lee, ACS Synth. Biol. (2018). 

[229] "Minimizing Clonal Variation During Mammalian Cell Line Engineering for Improved Systems Biology Data Generation", L. M. Grav, D. Sergeeva, J. S. Lee, I. M. de Mas, N. E. Lewis, M. R. Andersen, L. K. Nielsen, G. M. Lee, and H. F. Kildegaard, ACS Synth. Biol. 7:2148 (2018). 

[228] “Differential Expression of microRNAs in Recombinant Chinese Hamster Ovary Cells Treated with Sodium Butyrate Using Digital RNA Counting”, S. M. Lim, S. H. Park; J. H. Lee, S. H. Kim, J. Y. Kim, J. K. Min, G. M. Lee, and Y. G. Kim, J. Biotechnol. 283:37 (2018).

[227] “Improving the Production of Recombinant Human Bone Morphogenetic Protein-4 in CHO Cell Cultures by Inhibition of Its Undesirable Endocytic Mechanism”, C. L. Kim, M. Y. Jung, Y. S. Kim, J. W. Jang, G. M. Lee, Biotechnol. Bioeng. 115:2565 (2018).

[226] ”Untangling the Mechanism of 3-Methyladenine (3-MA) in enhancing the specific productivity: Transcriptomic analysis of a CHO cell line producing Fc-fusion protein treated with 3-MA”, E. Baek, J. S. Lee, G. M. Lee, Biotechnol. Bioeng. 115:2243 (2018).

[225] “CRISPR/Cas9-Multiplexed Editing of Chinese Hamster Ovary B4Gal-T1, 2, 3 and 4 Tailors N-Glycan Profiles of Therapeutics and Secreted Host Cell Proteins”, T. Amann, A. H. Hansen, S. Ko, G. M. Lee, M. R. Andersen, and H. F. Kildegaard, Biotechnol. J. 10:e1800111 (2018). 

[224] “Baicalein Reduces Oxidative Stress in CHO Cell Cultures and Improves Recombinant Antibody Productivity” T. K. Ha, A. H. Hansen, S. Kol, H. F. Kildegaard, and G. M. Lee, Biotechnol. J. (2018).

[223] “Co-Amplification of EBNA-1 and PyLT through dhfr-Mediated Gene Amplification for Improving Foreign Protein Production in Transient Gene Expression in CHO Cells” J. H. Lee, J. H. Park, S. H. Park, S. H. Kim, J. Y. Kim, J. K. Min, G. M. Lee, and Y. G. Kim, Appl. Microbiol. Biotechnol. (2018).

[222] “Comprehensive Characterization of Glutamine Synthetase-Mediated Selection for the Establishment of Recombinant CHO Cells Producing Monoclonal Antibodies” S. M. Noh, S. Shin, and G. M. Lee, Sci. Rep. (2018).

[221] “Simple and Robust N-Glycan Analysis Based on Improved 2-Aminobenzoic Acid Labeling for Recombinant Therapeutic Glycoproteins” Y. R. Jeong, S. Y. Kim, Y. S. Park, and G. M. Lee, J. Pharm. Sci. (2018).

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