Removed petri plate of mother colony from incubator and took pictures
Analyzed using ImageJ Software Colony Blob Count Tool
There were 67 colonies and an average area of 21182.97
Calibrated turbidimeter with distilled water, shows 0.357 NTU error so subtracted 0.357 NTU from raw data
Put 3 mL Luria broth into turbidimeter cuvette, inoculated with bacteria, recorded 3 turbidity measurements and took the average, transfer solution to corresponding sample vial
Repeat for Samples 44-60
Incubated vials at corresponding temperature
Will be ready to remove from incubator on 2/18/21