1/14/21

Removed Group B PET (Samples 31-60) from UVC light

Using forceps, dipped each PET strip into beakers with distilled water then ethanol, dried with Kimwipe, recorded mass in Excel

Placed PET strip to corresponding sample vial

Calibrated turbidimeter with distilled water, shows 0.226 NTU error so subtracted 0.226 NTU from raw data

Put 3 mL Luria broth into turbidimeter cuvette, inoculated with bacteria, recorded 3 turbidity measurements and took the average, transfer solution to corresponding sample vial

• • Repeat for Samples 6-10, 16-20, 26-43

  • Will be ready to remove from incubator on 2/12/21

  • Samples 31 and 32 have extreme turbidity readings because it was a different bottle of Luria broth and it might be expired

  • Only got to Sample 43 because agar plate of mother colony was pierced and couldn’t inoculate vials with enough bacteria for remaining 17 vials

Incubated vials at corresponding temperature

Inoculated new agar plate with original rehydrated culture vial in liquid suspension

• • Incubated at 30℃ and will remove on 1/19/21 for 4.5 day incubation period

  • Will inoculate remaining vials and measure turbidity on 1/19/21, don’t need to re mass PET strips because they are already in corresponding sterile vials