Bacteriology tests

Air sampling for culture

Blood and peritoneal fluid for culture and sensitivity

Bronchial alveolar lavage/washing for culture and sensitivity

Bronchial alveolar lavage/washing for microscopy

Cerebrospinal fluid (CSF) for Culture and sensitivity

Cerebrospinal fluid (CSF) for cryptococcal antigen

Cerebrospinal fluid (CSF) for Microscopy (Indian ink/ cell count/ gram stain)

Corneal scraping for culture and sensitivity

Corneal scraping for microscopy (Gram stain/Giemsa stain/KOH)

Ear swab for culture and sensitivity

Endocervical swab for culture and sensitivity

Eye swab for culture and sensitivity

Eye swab for microscopy

Fluids or Biomaterial (Sterility testing)

Gastric lavage for culture and sensitivity

High vaginal swab for culture and sensitivity

Nasal Swab (MRSA Screening)

Pus aspirate for culture and sensitivity

Sputum for culture and sensitivity

Sterile body fluids for culture and sensitivity

Sterile body fluids for microscopy cell count

Stool for culture and sensitivity

Stool for Clostridium difficile toxin A

Rectal swab for Vibrio cholera (RSVC)

Rectal swab for CRE screening

Throat swab (Tonsilitis/Diphtheria)

Tissue for culture and sensitivity

Tracheal aspirate for culture and sensitivity

Tracheal aspirate for microscopy

Urethral swab (MALE) for culture and sensitivity

Urethral swab (MALE) for microscopy

Urine for culture and sensitivity

Urine for microscopy

BACK

TEST (REF)

Air sampling for culture

SPECIMEN

Air sampling

VOLUME

CONTAINER

Blood Culture Agar (BA) and Sabouraud Dextrose Agar (SDA)

LTAT

NOTE

To request agar plates from Bacteriology Laboratory

Blood and peritoneal fluid for culture and sensitivity

SPECIMEN

Blood and Peritoneal Fluid only

VOLUME

Adult:

8-10ml

Pediatic / Neonates:

1-5ml

CONTAINER

Bactec BD (Aerobic/ Anaerobic)

Bactec Peads

LTAT

See note

Note

Positive culture results: 3-7 days, depends of Time of Detection (TTD) by incubation system.

Negative culture results : 5 days

Peritoneal fluid for culture and sensitivity also can be sent in sterile container

Bronchial alveolar lavage/washing for culture and sensitivity

SPECIMEN

Bronchial alveolar lavage / washing

VOLUME

CONTAINER

Sterile container

LTAT

3 days

Note

Bronchial alveolar lavage/washing for microscopy

SPECIMEN

Bronchial alveolar lavage/ washing

VOLUME

CONTAINER

Sterile container

LTAT

24 hours

Note

On request

Cerebrospinal fluid (CSF) for Culture and sensitivity

SPECIMEN

Cerebrospinal fluid (CSF)

VOLUME

1-3 ml

CONTAINER

Sterile Bijou bottle

LTAT

3 days

NOTE

Cerebrospinal fluid (CSF) for cryptococcal antigen

SPECIMEN

Cerebrospinal fluid (CSF)

VOLUME

1-3 ml

CONTAINER

Sterile Bijou bottle

LTAT

1 hour

NOTE

Cerebrospinal fluid (CSF) for Microscopy (Indian ink/ cell count/ gram stain)

SPECIMEN

Cerebrospinal fluid (CSF)

VOLUME

1-3 ml

CONTAINER

Sterile container

( Bijou bottle)

LTAT

1 hour

NOTE

Corneal scraping for culture and sensitivity

SPECIMEN

Corneal scraping

VOLUME

CONTAINER

Culture plate

LTAT

4 days

NOTE

To obtain culture plate from the laboratory (Blood Agar, Chocolate Agar)

Corneal scraping for microscopy (Gram stain/Giemsa stain/KOH)

SPECIMEN

Corneal scraping

VOLUME

CONTAINER

Smear on slide

LTAT

See note

NOTE

Upon request

Gram stain (LTAT 1 hour)

KOH and Giemsa (LTAT 24 hour)

Ear swab for culture and sensitivity

SPECIMEN

Ear swab

VOLUME

CONTAINER

Amies transport media

LTAT

3 days

NOTE

Endocervical swab for culture and sensitivity

SPECIMEN

Endocervical swab

VOLUME

CONTAINER

Amies transport media with charcoal (for suspected Gonorrhea)

LTAT

4 days

NOTE

Eye swab for culture and sensitivity

SPECIMEN

Eye swab

VOLUME

CONTAINER

Amies transport media

LTAT

3 days

NOTE

Eye swab for microscopy

SPECIMEN

Eye swab

VOLUME

CONTAINER

Smear on slide

LTAT

24 hours

NOTE

Microscopy for Gram stain (upon request)

Fluids or Biomaterial (Sterility testing)

SPECIMEN

Fluids or Biomaterial

VOLUME

5-10 ml

CONTAINER

Sterile container

LTAT

3 days

NOTE

Gastric lavage for culture and sensitivity

SPECIMEN

Gastric lavage

VOLUME

5-10 ml

CONTAINER

Sterile container

LTAT

3 days

NOTE

High vaginal swab for culture and sensitivity

SPECIMEN

High Vaginal Swab

VOLUME

CONTAINER

Amies transport media

LTAT

3 days

NOTE

Nasal Swab (MRSA Screening)

SPECIMEN

Nasal Swab

VOLUME

CONTAINER

Amies transport media

LTAT

3 days

NOTE

Pus aspirate for culture and sensitivity

SPECIMEN

Pus aspirates

VOLUME

CONTAINER

Sterile container

LTAT

3 days

NOTE

If anaerobic organism is suspected, to put sample in thioglycollate broth (to obtain from laboratory).

Sputum for culture and sensitivity

SPECIMEN

Sputum

VOLUME

CONTAINER

Sterile container

LTAT

3 days

NOTE

Preferably early morning sputum

Sterile body fluids for culture and sensitivity

SPECIMEN

Pleural fluid
Pericardial fluid
Peritoneal fluid
Synovial fluid
Intraocular fluid

VOLUME

CONTAINER

Sterile container

LTAT

3 days

NOTE

Sterile body fluids for microscopy cell count

SPECIMEN

Pleural fluid
Pericardial fluid
Peritoneal fluid
Synovial fluid
Intraocular fluid

VOLUME

CONTAINER

Sterile container

LTAT

24 hours

NOTE

Upon request

Stool for culture and sensitivity

SPECIMEN

Stool

VOLUME

CONTAINER

Sterile container

LTAT

3 days

NOTE

Stool for Clostridium difficile toxin A

Faeces should be loose/watery to conform to the shape of the container. Formed faeces will be rejected unless the requisition indicates the patient may have pseudomembranous colitis.

Rectal swabs are not an acceptable alternative and will not be tested for toxin.
Repeated test within 7 days will be rejected

SPECIMEN

Stool

VOLUME

CONTAINER

Sterile container

LTAT

24 hours

NOTE

Rectal swab for Vibrio cholera (RSVC)

SPECIMEN

Rectal swab

VOLUME

CONTAINER

Alkaline peptone water

LTAT

3 days

NOTE

Rectal swab for CRE screening

SPECIMEN

Rectal swab

VOLUME

CONTAINER

Amies transport media

LTAT

3 days

NOTE

Throat swab (Tonsilitis/Diphtheria)

SPECIMEN

Throat swab

VOLUME

CONTAINER

Amies transport media

LTAT

3 days

NOTE

Tissue for culture and sensitivity

SPECIMEN

Tissue

VOLUME

CONTAINER

Sterile container

LTAT

3 days

NOTE

Collect sample using aseptic technique.

If anaerobic organism is suspected, to put sample in thioglycollate broth (to obtain from laboratory)

Tracheal aspirate for culture and sensitivity

SPECIMEN

Tracheal aspirate

VOLUME

CONTAINER

Sterile container

LTAT

3 days

NOTE

Tracheal aspirate for microscopy

SPECIMEN

Tracheal aspirate

VOLUME

CONTAINER

Sterile container

LTAT

24 hours

NOTE

Urethral swab (MALE) for culture and sensitivity

SPECIMEN

Urethral swab

VOLUME

CONTAINER

Amies transport media

LTAT

3 days

NOTE

Urethral swab (MALE) for culture and sensitivity

SPECIMEN

Urethral swab

VOLUME

CONTAINER

Amies transport media with charcoal (for suspected Gonorrhea)

LTAT

3 days

NOTE

Urethral swab (MALE) for microscopy

SPECIMEN

Urethral swab

VOLUME

CONTAINER

Amies transport media

LTAT

24 hours

NOTE

Urine for culture and sensitivity

A. Midstream urine

Male
1. 1. Cleanse the glands and penis with soapy and water.
  2. Rinse area with wet gauze pads
  3. While holding the foreskin retracted, begin voiding.
  4. After several milliliters has passed, collect a midstream portion without stopping the flow of urine.
Female
1. 1. Thoroughly cleanse the urethral area with soap and water.
  2. Rinse area with wet gauze.
  3. While holding the labia apart, begin voiding.
  4. After several milliliters has passed, collect a midstream portion without stopping the flow of urine.

B. Suprapubic aspirate (SPA)

Note: SPA is useful in determining urinary infection in adults in which the results from routine procedures are equivocal but the diagnosis is critical. Also, useful for paediatric patients when clean-catch urine is difficult to obtain.

Before SPA, the patient should force fluids until bladder is full.
Shave and disinfect the suprapubic skin overlying the urinary bladder.
Aspirate urine from the bladder by using a needle aspiration technique.

C. Catheter urine

Catheters are placed in patients who are unable to pass urine

Clean the catheter collection port with 70% alcohol wipe
Using sterile technique, puncture the collection port with a needle attached to a syringe. Do not collect the urine from the bag.
Aspirate urine and place it in sterile container.
Send specimen immediately to the laboratory.

SPECIMEN

Urine

VOLUME

5-10 ml

CONTAINER

Sterile container or Boricon bottle

LTAT

3 days

NOTE

Use Boricon bottle (if delivery of more than 2 hours is anticipated)

Urine for microscopy

SPECIMEN

Urine

VOLUME

5-10 ml

CONTAINER

Sterile container or Boricon bottle

LTAT

24 hours

NOTE

Use Boricon bottle (if delivery of more than 2 hours is anticipated)

BACK

TEST (REF)

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