Tests-Haematology

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TESTS (REF)

BCR-ABL quantitative assay

Specimen

Fresh venous blood

Volume

10.0 mL (4 tubes)

Container

EDTA (K2)

LTAT

7 days

Note

Monday-Tuesday.

Sample not more than 24 hours from collection time.

Guide

Bone marrow aspirate

- PBF sample is required to be sent together.
- Fill in the request forms with relevant CLINICAL INFORMATION for immunophenotyping, cytogenetic and molecular study for all cases.
- For BMA cases from OUTSTATION, please include
  1. Minimum 5 slides
  2. Recent FBC and PBF slide
  3. Immunophenotyping analysis sample (if required)

Specimen

Bone marrow

Volume

Slides

Container

Slides

LTAT

5 days

Note

Please call ext: 7329 to arrange for an appointment (working days only).

CD4 CD8 enumeration

Specimen

Fresh venous blood (not more than 24 hours from collection time)

Volume

2.5-3.0 mL

Container

EDTA (K2)

LTAT

7 days

Note

By appointment Ext: 7329

Monday-Wednesday

Last sample must reach non-routine hematology lab by 3.30pm.

D-Dimer

Specimen

Fresh venous blood

Volume

2.7 mL

*To fill up to marked level on tube

Container

3.2% Trisodium citrate tube

LTAT

90 mins

Note

To send immediately or within 30 minutes after blood collection.

Erythrocytes sedimentation rate

Specimen

Fresh venous blood

Volume

Minimum 1.0 mL

Container

EDTA (K2)

LTAT

24 hours

Note

To send immediately after blood collection.

Sample stability: 4 hours after collection.

DO NOT share form with other test.

Office hours only.

Factor assay (VIII and IX)

Specimen

Fresh venous blood

Volume

5 tubes

2.7 mL

*To fill up to marked level on tube

Container

3.2% Trisodium citrate tube

LTAT

14 days

Note

By appointment Ext: 7329.

To send immediately or within 30 minutes after blood collection.

Must have a complete and relevant clinical summary.

Office hours only.

FORM

Factor inhibitor assay (VIII and IX)

Specimen

Fresh venous blood

Volume

5 tubes

2.7 mL

*To fill up to marked level on tube

Container

3.2% Trisodium citrate tube

LTAT

14 days

Note

By appointment Ext: 7329.

To send immediately or within 30 minutes after blood collection.

Must have a complete and relevant clinical summary.

Office hours only.

FORM

Full Blood Count

Specimen

Fresh venous blood

Volume

2.5 – 3.0 mL

(minimum 1mL)

Container

EDTA (K2)

LTAT

Urgent 45 min

Non urgent 3 hours

Note

To send immediately or within 30 minutes after blood collection.

Glucose-6- phosphate Dehydrogenase (G6PD) fluorescent spot test.

Specimen

Fresh venous blood (not more than 24 hours from collection time)

Volume

2.5 – 3.0 mL or spot blood on filter paper

Container

EDTA (K2) or filter paper

LTAT

24 hours

Note

Please provide relevant clinical summary.

For urgent requests after office hours, please send to Integrated Laboratory.

Hb analysis

- Hb Electrophoresis is NOT indicated as first-line test for investigation of anaemia or hypochromic microcytic anaemia in adults, except in cases with hepatosplenomegaly to suggest thalassemia intermedia.
- Please exclude iron deficiency first either biochemically or by trial of iron therapy in younger patients.
- In accordance to National screening policy, Hb electrophoresis will only be performed in cases with MCV <80 fl or MCH <27. Hence, rare milder cases of silent or MCH or MCV normal Thalassemia will not be detected.
- Post transfusion sample will not give accurate result. Kindly send Hb analysis 3 months post-transfusion.
- Neonatal less than 1 year of age has physiologically high HbF. Kindly discuss with pathologist if Hb analysis is required.
- For sample from OUTSTATION, laboratory personnel must attach
  1. Blood film smear.
  2. Recent FBC result.
- All Hb analysis request must be registered in iThal system.

Specimen

Fresh venous blood

Volume

2.5 – 3.0 mL

Container

EDTA (K2)

LTAT

4 weeks

Note

FORM

Immature platelet fraction (IPF)

Specimen

Fresh venous blood

Volume

2.5 – 3.0 mL

(minimum 1mL)

Container

EDTA (K2)

LTAT

3 hours

Note

Guide

Leukaemia immunophenotyping analysis

Specimen

Bone marrow or fresh venous blood

Volume

2 tubes

2.5-3.0 mL

Container

EDTA (K2)

LTAT

3 days

Note

By appointment Ext: 7329.

To send immediately.

Please include bone marrow or peripheral blood smear.

Lupus anticoagulant

- It is MANDATORY to fill in the form with relevant clinical history and drug history (i.e. anticoagulant usage / factor replacement therapy including date and time of last dose given).
- Patient on anticoagulant should be discontinued as below:
  1. Warfarin 2 weeks
  2. Un-fractionated heparin (UFH) 24 hours
  3. Low molecular weight heparin (e.g; clexane) 12-24 hours
  4. Direct oral anticoagulant (e.g; dabigatran, apixaban) 72 hours
- For thrombophilia testing, please use this guideline
- Testing should be delayed until at least 6 weeks after the acute event.
- Lupus anticoagulant testing during pregnancy does not exclude or confirm a diagnosis of APS. Further read.

Specimen

Fresh venous blood

Volume

5 tubes

2.7 mL

*To fill up to marked level on tube

Container

3.2% Trisodium citrate tube

LTAT

14 days

Note

By appointment Ext: 7329.

To send immediately or within 30 minutes after blood collection.

Must have complete and relevant clinical summary.

Office hours only.

FORM

Anti cardiolopin and anti B2GP1 antibodies send separately to Serology HQE using plain tube.

Mixing test

Specimen

Fresh venous blood

Volume

5 tubes

2.7 mL

*To fill up to marked level on tube

Container

3.2% Trisodium citrate tube

LTAT

14 days

Note

By appointment Ext: 7329.

To send immediately or within 30 minutes after blood collection.

Must have complete and relevant clinical summary.

Office hours only.

FORM

Peripheral blood film

Specimen

Fresh venous blood

Volume

2.5 – 3.0 mL

Container

EDTA (K2)

LTAT

24 hours (urgent)

5 working days

Note

To send immediately or within 30 minutes after blood collection.

MUST provide complete clinical summary.

Do not share form with other tests.

For URGENT cases/ after office hours, please discuss with MO/ Pathologist on-call.

Platelet aggregation test

Specimen

Fresh venous blood

Volume

4 tubes

2.7 mL

*To fill up to marked level on tube

Container

FOUR 3.2% Trisodium citrate tube and ONE EDTA (K2)

LTAT

21 days

Note

The test can only be requested by a Clinical Haematologist or Paediatric Haemato-oncologist.

The appointment date can be taken from Haematology Laboratory Ext: 7329 (to consult a haematopathologist).

Guide

FORM

PT APTT Fibrinogen

Specimen

Fresh venous blood

Volume

2.7 mL

*To fill up to marked level on tube

Container

3.2% Trisodium citrate tube

LTAT

3 hours

Note

To send immediately or within 30 minutes after blood collection.

Reticulocytes

Specimen

Fresh venous blood

Volume

2.5 – 3.0 mL

(minimum 1mL)

Container

EDTA (K2)

LTAT

3 hours

Note

Test criteria:

1. Hb< 7 g/dL for routine FBC (first time) and PBF

2. Significant down going Hb level/ suspected hemolysis.

Request for retic count through MO/pathologist.

Urine Hemosiderin

Specimen

Fresh urine *(Not more than 8 hours)

Volume

10.0 mL

Container

Sterile container

LTAT

24 hours

Note

Preferably first urine of the day.

Von Willebrand factor antigen and activity

Specimen

Fresh venous blood

Volume

5 tubes

2.7 mL

*To fill up to marked level on tube

Container

3.2% Trisodium citrate tube

LTAT

14 days

Note

By appointment Ext: 7329.

To send immediately or within 30 minutes after blood collection.

Must have a complete and relevant clinical summary.

Office hours only.

FORM

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TESTS (REF)

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