Metabarcoding and molecular taxonomy

Reliable and comprehensive, but rapid and cost-effective methods for monitoring freshwater ecosystems are encouraged due to the increasing threats of biological degradation faced by freshwaters worldwide. We studied metabarcoding comparison of stream macroinvertebrates for biodiversity and monitoring assessment, and compared diversity metrics and assessment by metabarcoding and morphological analysis. Potential next steps will be the improvements in the detection of rare samples by increasing the sensitivity of the methods. 


Publications

Kolcsar L.P., Paramond N, Kato D, Gamboa M, Imada Y, Kato M, Watanabe K. 2022. Notes on the taxonomic status and distribution of some Cylindrotomidae (Tipuloidea, Diptera), with emphasis on Japanese species. Zookeys. 1083: 13-88.

Muranyi D,  Kovacs T, Gamboa M, Watanabe K.  2021. Loosing the  generic character: description of the larva of Isoperla vevcianensis Ikonomow, 1980 (Plecoptera: Perlodidae).  Zootaxa. 5082: 541-552.

Levante-Peter K, Kato D, Gamboa M, Watanabe K. Revision of Japanese species of Nipponomyia Alexander, 1924 (Diptera, Pediciidae).  Zookeys. 1000: 71-105.

Mo RR, Gamboa M, Watanabe K, Wang GQ, Li WH, Yang D, Muranyi D. 2020. A remarkable new genus and species of Nemourinae (Plecoptera, Nemouridae) from Sichuan, China, with systematic notes on the related genera. Plos one, 15(3): e0229120.

Muranyi D, Gamboa M, Watanabe K. 2020. A new species of Protonemoura Kempny, 1898 (Plecoptera: Nemouridae) from the Ishizuchi range, Shikoku, Japan. Zootaxa. 4718: 57-66.

Serrana J, Miyake Y, Gamboa M, Watanabe K. 2019. Comparison of DNA metabarcoding and morphological identification for stream macroinvertebrate biodiversity assessment and monitoring. Ecological indicators. 101: 963-972.

Li W, Muranyi D, Gamboa M, Yang D and Watanabe K. 2017. New species and records of Leuctridae (Plecoptera) from Guangxim China, on the basis of morphological and molecular data, with emphasis on Rhopalopsole. Zootaxa 4243(1): 165-176.

Muranyi D, Gamboa M, Vera A. 2016. Lost and found: the Plecoptera types of Blanchard and Mabile, with further contributions to Chilean stoneflies. Zootaxa 4200(4): 544-560.

Gamboa M, MT Monaghan. 2015. Association of adult female and male stoneflies (Plecoptera) from an Alpine river using wing morphometrics and mitochondrial DNA. Aquatic insects 36(1):1-8.

Murányi D, M Gamboa, KM Orci. 2014. Zwicknia gen. n. for the Capnia bifrons species group, with the description of three new species and a synopsis of the West Palaearctic and Nearctic genera of Capniidae (Plecoptera). Zotaxa 3812(1):001-082.

Graf W, S Popijač, A Previšić, M Gamboa, M Kučinić. 2012. Contribution to the knowledge of Siphonoperla in Europe (Plecoptera: Chloroperlidae): Siphonoperla korab nov. sp. Zootaxa 3164:41-48.