Overview of AIF1 Antibody

Overview of AIF1 Antibody

AIF1 antibody is a rabbit polyclonal antibody raised against AIF1. It has been selected for its ability to recognize AIF1 in immunohistochemical staining and western blotting. Allograft-inflammatory factor 1 (AIF-1) is a 17-kDa cytoplasmic protein with two calcium-binding EF-hand motifs. It is also known as daintain and Iba1 (aa 45-80 and aa 81-115). The proinflammatory response to ischemia, tissue injury, and atherosclerosis is mediated by AIF-1, which is expressed in activated macrophages. In these locations, it is also expressed in vascular smooth muscle and endothelial cells, where it promotes vascular remodeling and the development of new vessels. During transplant rejection, AIF-1 is increased in allografted tissue. AIF1 antibody recognizes epitopes at the C-terminal region of AIF1 and is expected to recognize AIF1 isoform 1 and isoform 3 formed by alternative splicing. AIF1 was first identified in rat cardiac allografts with chronic rejection.

AlF1 Functions

Neuronal function, inflammatory responses, allograft rejection, autoimmunity, and macrophage activation and function have all been linked to AlF1. In autoimmune diseases such as experimental autoimmune encephalomyelitis, neuritis, and uveitis models, it has also been found to be expressed in macrophages and microglial cells. It can also be utilized to distinguish malignant reticulosis (AIF1 positive) from astrocytomas in malignancy (negative). AIF1 displays cytoplasmic and membranous positivity in immune cells in normal tissues, with notably high expression in lung macrophages.