AIF1 Structure

AIF1 Structure

AIF1 (AIF1 antibody) is a 147-amino-acid 17-kDa actin-binding protein that plays a role in macrophage activation, vascular smooth muscle cell proliferation, and T-lymphocyte proliferation. Anti-AIF1 antibody from goats detects epitopes in AIF1's C-terminal domain and is likely to recognize AIF1 isoforms 1 and 3, which are produced through alternative splicing.

The molecular mass of recombinant human AIF1 is predicted to be 18.5 KDa, with 162 amino acids. Under decreasing circumstances, it migrates as an approximately 18 KDa band on SDS-PAGE.

AIF1 and AIF1-like are two paralogs of the allograft inflammatory factor (AIF) gene family that has been found (AIF1L). AIF1 and AIF1L, the encoded proteins, are 80 percent identical in sequence and have a conserved tertiary structure. While research in human populations has found linkages between AIF1 and metabolic illnesses like obesity and diabetes, no such links have been found with AIF1L. We hypothesized that AIF1L may play a role in metabolic diseases based on structural similarities and investigated it using animal models.

The human AIF1 protein is 98 percent identical to that of the rat. Macrophages and neutrophils both express AIF1. In rat macrophages, IFN-g increases the expression of AIF1 transcripts. AIF1 is expressed in a subpopulation of CD68(+) macrophages in the interstitial and perivascular regions of human heart allografts, as well as in human macrophage-like cell lines. IFN-g, IL1b, and T-cell conditioned media to stimulate AIF1 production in quiescent human vascular smooth muscle cells. Overexpression of AIF1 in human VSMCs causes these cells to develop faster. AIF1 is expressed in the rat mammary gland and ventral prostate tissues during apoptosis. In rat and human gliomas, some tumor-associated activated macrophages and microglial cells express Allograft Inflammatory Factor 1.