1. Surveillance of Antimicrobial Resistance (AMR) in food and environmental samples – isolation and molecular characterization of non-pathogenic microorganisms (from food and water bodies); molecular profiling of AMR genes and virulence and study of their horizontal transfer; metagenomic sequencing (HT-NGS) and analysis.
2. Silver Nanostructures in combination with antibiotics for combating AMR microorganisms – different shapes and sizes of nanoparticles, synthesized by different methods, to be combined with antibiotics against ESKAPE and other resistant microorganisms, study of their antimicrobial action and testing cytotoxicity.
3. Oxidative stress adaptations in Escherichia coli.
4. FEAMR© surveillance database.
5. CRSIPR based studies for AMR.
1. Title of the Project: A study on the prevalence and molecular profiling of virulence genes in multiple antibiotic resistant Escherichia coli isolated from retail food
Name of the Funding Agency: SERB, DST
Duration: May 2019-March 2023
Amount (in Rs.): 42,82,360*
Overheads (in Rs.): 3,89,000
*Includes financial provision for SSR & Lab Visit (details provided at Contact Us Page)
2. Title of the Project: Isolation and molecular characterization of antibiotic resistance in non-pathogenic organisms from food
Name of the Funding Agency: BRNS, DAE
Duration: April 2013-December 2016
Amount (in Rs.): 25,38,350
Overheads (in Rs.): 3,75,000
3. Title of the Project: Isolation and Molecular Characterization of Antibiotic Resistant Microorganisms from Different Water Bodies
Name of the Funding Agency: DST-PURSE Scheme, UoM
Duration: April 2017 onwards
Amount (in Rs.): 7,30,000 (received till date)
Mishra, M., Ballal, A., Rath, D. and Rath, A., 2024. Novel Silver Nanoparticle-Antibiotic Combinations as Promising Antibacterial and Anti-biofilm Candidates against Multiple-Antibiotic Resistant ESKAPE Microorganisms. Colloids and Surfaces B: Biointerfaces, p.113826. https://doi.org/10.1016/j.colsurfb.2024.113826
Ghadigaonkar, D. and Rath, A., 2023. Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes. JoVE (Journal of Visualized Experiments), (193), p.e63934. https://www.jove.com/t/63934/isolation-identification-waterborne-antibiotic-resistant-bacteria
Mishra, M., Kadam, P., Doshi, B., Saravya, J. and Rath, A., 2022. FEAMR: A Database for Surveillance of Food and Environment-Associated Antimicrobial Resistance. Interdisciplinary Sciences: Computational Life Sciences, 14(4), pp.833-840. https://link.springer.com/article/10.1007/s12539-022-00534-y
Dhawale, A., Bindal, G., Rath, D., & Rath, A. (2021). DNA repair pathways important for the survival of Escherichia coli to hydrogen peroxide mediated killing. Gene, 768, 145297. https://www.sciencedirect.com/science/article/abs/pii/S0378111920309665
1. Registered Copyright: Literary work entitled “FEAMR: Food and Environment associated Antimicrobial Resistance Database” (Registration Number: L-96624/2020)
2. Patent award entitled “Biological Synthesis Process of Silver Nanoparticles and Application as Antimicrobial Agent against Multiple Antibiotic Resistant Bacteria” (Patent Number 458867)
The research work appeared as a short story in a televised show, “Science Monitor” on “Rajya Sabha TV”, Vigyan Prasar, an autonomous Institute of the Department of Science & Technology, aired on 5th August 2017. https://sites.google.com/view/amrlabudbtuom/gallery/our-research-on-tv?authuser=0 (full video available at https://www.youtube.com/watch?v=SPnSDrVqACc)
“Hindustan Times”https://www.hindustantimes.com/cities/fish-bacteria-can-resist-drugs-used-to-treat-tb-malaria-reveals-mumbai-university-study/story-RKEezTi65fzchtZA2YQieN.html published on 23rd April 2018.
“The Hindu” http://www.thehindu.com/todays-paper/tp- national/tp-other states/mumbai- study-shows-drug-resistant-bacteria-in-chicken-moong-beans/article19213505.ece published on 5th July, 2017.
“Outlook”https://www.outlookindia.com/website/story/scientists-discover-antibiotic-resistant-bugs-in-raw-chicken-sprouts/299659 published on 20th July, 2017.
“Biotech Times”https://biotechtimes.org/2017/07/20/study-finds-antibiotics-bugs-chicken-sprouts/ published on 20th July, 2017.
“Indian Science Journal” http://www.indiansciencejournal.in/health-news/raw-chicken-and-sprouted-beans-in-mumbai-unhealthy-206806 published on 20th July, 2017.
“India Science Wire” http://vigyanprasar.gov.in/isw/antibiotic_sea_fishes_story.html published on 20th December, 2017.
“India Water Portal” http://hindi.indiawaterportal.org/node/66772 published on 20th December, 2017.
“Mongabay”https://india.mongabay.com/2018/01/11/mumbai-fish-harbour-high-levels-of-antibiotic-resistant-bacteria/ published on 11th January, 2018.
There are a total of 214 submissions from our lab till date.
The NCBI BioProject Accession no: PRJNA639905 contains all submissions done by the AMR Lab to NCBI (https://www.ncbi.nlm.nih.gov/) till date, which can be accessed at https://www.ncbi.nlm.nih.gov/bioproject/PRJNA639905.
A. Gene sequences of Antibiotic Resistant microorganism isolated from food samples:
1. Food Source: Chicken
No. of isolates: 50
Accession Numbers: KX355642 – KX355691
Publication date on NCBI: 26-JUNE-2016
2. Food Source: Mung sprout
No. of isolates: 50
Accession Numbers: KX355692 – KX355741
Publication date on NCBI: 26-JUNE-2016
3. Food Source: Marine fish
No. of isolates: 50
Accession Numbers: KX300038 – KX300086, KY432753
Publication date on NCBI: 26-JUNE-2016, 14-JULY-2017
B. Antibiotic Resistant gene sequences amplified from metagenomic DNA:
No. of genes: 13
Accession Numbers: KY798891-KY798897, KU573047-KU573052
Publication date on NCBI: 09-MAY-2017, 09-FEB-2017
C. Antibiotic Resistant gene sequences amplified from transconjugants:
No. of genes: 3
Accession Numbers: KY924468-KY924470
Publication date on NCBI: 12-JUN-2017
D. Antibiotic Resistant gene sequences amplified from isolates:
No. of genes: 11
Accession Numbers: KY867437-KY867447
Publication date on NCBI: 19-APR-2017
National Seminar-cum-Event on Biotechnology Entrepreneurship (NSBTE) in India: Recent Trends and Challenges held on 26th March 2018.
BIOINNOVA National Conference in collaboration with Thakur College, Kandivali held on 10th February 2018.
National Science Day held on 28th February 2017 & 2018.
'Tackling Anti-microbial Resistance (AMR) from One Health Perspective' delivered on 4th-5th March 2024, during the Two day International Conclave on Antimicrobial Resistance, organised by the DBT-CTEP, sponsored and organized by the Centre of Excellence in Life Sciences, PSGR Krishnammal College for Women, Coimbatore, India.
'Seafood Safety from One Health Perspective' Lecture during ICAR-CIFE organized and DST-SERB sponsored two-day National Seminar "Seafood Safety and Public Health" on 4th & 5th January, 2024.
Understanding AMR through Metagenomic Approaches - ICAR Sponsored Winter School (9-29th January 2023) on Antimicrobial resistance in food fish: Challenges and Mitigation, conducted by CIFE, Mumbai – 25th January 2023
Metagenomics in Clinical Microbiology - PG Assembly (Western Region) of IAMM at Seth G.S. Medical College & K.E.M. Hospital, Mumbai – 29th July 2019.
1. Title of the Paper presented: Antibacterial activity of differently shaped ZnO nanostructures on UTI causing microorganisms
Title of Conference/ Seminar: The International Conference on Reproductive Health with Emphasis on Occupational, Environmental and Lifestyle Factors –ISSRF 2016
Date of the event: 18 to 20th February 2016 (Date of the Oral presentation 20th February 2016)
Organized by: National Institute of Occupational Health (ICMR) and ISSRF
2. Title of the Paper presented: Morphological dependence of ZnO nanostructures on antibiotic activity
Title of Conference/ Seminar: 6th World Congress on Biotechnology
Date of the event: 05 to 07th October 2016
Organized by: OMICS International Conferences
3. Title of the Paper presented: Prevalence of Antibiotic Resistant Micro – organisms in urinary tract infections and Sexually Transmitted Diseases
Title of Conference/ Seminar: ‘Science and Technology for Women Development.’(Women’s Science Congress under 102nd Indian Science Congress Association)
Date of the event: 3rd-7th January 2015 (Date of the Oral presentation 5th January 2015) Organized by: Indian Science Congress Association
4. Title of the Paper presented: Prevalence and Molecular characterization of antibiotic resistant micro-flora in ready-to-eat sprouts
Title of Conference/ Seminar: Science and Technology for Human Development.
Date of the event: 3rd-7th January 2015 (Date of the Oral presentation 7th January 2015) Organized by: Indian Science Congress Association
5. Title of the Paper presented: A study on the prevalence and characterization of antibiotic resistant micro-organisms in fish
Title of Conference/ Seminar: 83rd Annual meeting of the Society of Biological Chemists (India) and symposium on Evolution: Molecules to Life
Date of the event: 18-21 December, 2014
Organized by: KIIT University, Institute of Life Sciences and National Institute of Science Education & Research)
6. Title of the Paper presented: Response of Escherichia coli mutants to oxidative stress and the evaluation of the DNA damage by TUNEL assay.
Title of Conference/ Seminar: XL All India Cell Biology Conference & International Symposium on “Functional Genomics and Epigenomics”
Date of the event: November 17-19, 2016
Organized by: Indian Society of Cell Biology (ISCB)
7. Title of the Paper presented: Involvement of DNA Helicases in Response to Oxidative Stress Damage in Escherichia coli
Title of Conference/ Seminar: XXXXII Annual Conference of Environmental Mutagen Society of India (EMSI) & National Conference on “Environmental Mutagenesis: Integration of Basic Biology & Omics to improve Human Health”
Date of the event: 25-27 January, 2018
Organized by: Environmental Mutagen Society of India (EMSI) &Bhabha Atomic Research Centre (BARC), Mumbai
8. Title of the Paper presented: Incidence, antibiotic resistance and molecular characterization of micro-organisms isolated from chicken
Title of Conference/ Seminar: DAE-BRNS LIFE SCIENCES SYMPOSIUM (LSS-2015) Advances in Microbiology of Food, Agriculture, Health and Environment
Date of the event: February 3-5, 2015
Organized by: Bioscience Group, BARC, Mumbai
9. Title of the Paper presented: Food commensals: Vast pool of antibiotic resistance and mode of its dissemination
Title of Conference/ Seminar: International Conference on “Recent Trends in Environment, Technology & Economy – SWAYAZAS -2017
Date of the event: 10th – 11th February 2017
Organized by: SIES(Nerul) College of Arts, Science and Commerce (SIESASCN) in association with Bombay Natural History Society
10. Title of the Paper presented: Horizontal gene transfer: Common mechanism of antibiotic resistance spread in food
Title of Conference/ Seminar: National Conference on Food, Nutrition & Healthcare
Date of the event: 10th February 2018
Organized by: Thakur College of Science & Commerce, Mumbai- Department of Biotechnology & IQAC, In collaboration with Department of Biotechnology, University of Mumbai
11. Title of the Paper presented: An Investigative Study of Antibacterial Potential of Streptococcus pneumoniae Synthesized Silver Nanoparticles on Antibiotic Resistant Micro-organisms
Title of Conference/ Seminar: International Conference on Nanoscience and Nanotechnology – “NanoSciTech 2017
Date of the event: 8th – 10th November 2017
Organized by: Panjab University, Chandigarh
12. Title of the Paper presented: Biochemical and Molecular Analysis of Antibacterial Activity of Streptococcus pneumoniae Synthesized Silver Nanoparticles
Title of Conference/ Seminar: International Congress of Cell Biology 2018 The Dynamic Cell: Molecules and Networks to Form and Function
Date of the event: 27th – 31st January 2018
Organized by: Centre for Cellular and Molecular Biology (CCMB), Hyderabad
13. Title of the Paper presented: Food Associated Antibiotic Resistance and Its Implications to Human Health – Invited Talk
Title of Conference/ Seminar: International Conference on Interdisciplinary Approaches Towards Environmental Sustainability (IAETS- 2018)
Date of the event: 17th November 2018
Organized by: Chhatrapati Shivaji Maharaj University, Panvel
14. Title of the Paper presented: A study on antibiotic resistant Escherichia coli isolated from retail foods in Mumbai, India
Title of Conference/ Seminar: World Congress on Infectious Diseases and Antibiotics-2018
Date of the event: 28th and 29th November 2018
Organized by: J.N.TATA Auditorium, Indian Institute of Science, Bengaluru, Karnataka
15. Title of the Paper presented: Elucidation of Anti-microbial Activity of Biologically Synthesized Silver Nanoparticles against Multiple Antibiotic Resistant Micro-organisms
Title of Conference/ Seminar: Society of Biological Chemists (SBC) Annual Meeting 2018- Mumbai Chapter
Date of the event: 13th October, 2018
Organized by: ICT, Mumbai
16. Title of the Paper presented: Elucidation of Mechanism of Antibacterial Activity of Microbially Synthesized Nanoparticles
Title of Conference/ Seminar: International Conference on Advances in Materials Science & Applied Biology (AMSAB)
Date of the event: 8th-10th January 2019
Organized by: NMIMS, Mumbai
17. Title of the Paper presented: Analysis of co-resistance associated Multiple Antibiotic Resistance in microorganisms isolated from retail food
Title of Conference/ Seminar: 10th DAE-BRNS Life Science Symposium -2019 (LSS-2019) on "Molecular and Cellular Responses to Stressors and Cancer Therapeutics”
Date of the event: March 28-30, 2019
Organized by: BARC, Mumbai
18. Title of the Paper presented: Metagenomic analysis as a complementary surveillance method to conventional culturable technique for analysis of Antimicrobial Resistant (AMR) microorganisms across Mumbai region
Title of Conference/ Seminar: XLIII All India Cell Biology Conference (AICBC)
Date of the event: 19-21 Dec 2019
Organized by: IISER Mohali
19. Title of the Paper presented: Dissemination of Antibiotic Resistance Traits from sea food to humans via Horizontal Gene Transfer
Title of Conference/ Seminar: International Conference on Emerging areas of Biosciences and Biomedical Technologies (eBBT-2)
Date of the event: Feb 7-9, 2020
Organized by: IIT Indore
20. Title of the Paper presented: Contribution of anthropogenic activity and Climate change towards development of anti-microbial resistance
Title of Conference/ Seminar: National Conference on Climate Change (NCCC)
Date of the event: March 12-13, 2020
Organized by: Utkal University, Bhubaneshwar