Expertise available with the AMR Lab
Molecular Biology Techniques, Microbiological techniques, Bioinformatics, Nanotechnology, Metagenomics, Oxidative stress and DNA Repair study related techniques.
Services available with the AMR Lab
Sample Testing: antimicrobial activity testing/Efficacy studies/Validation studies of drugs/ APIs/antimicrobial agents etc. based on AST/MIC/disc diffusion/ Agar Cup/Zone of Inhibition/Fractional Inhibitory Concentration Test (FIC test: It is the test to estimate the interaction between two or more drugs intended to be used in combination. The purpose of this method is to test two different antimicrobial agents or new antimicrobial agents in combination with existing for determining the Synergistic effect, Additive effect and/or Antagonism) etc., in plate/tube/microtitre plate formats, on standard/reference microbial strains (like ATCC strains) or on our own repository of AMR microbial strains isolated from various ecosystems (especially from animal, food and environment samples).
Collaborative Research work: We welcome collaborations involving any aspect of AMR, including Isolation and detection of antibiotic resistant bacteria from environment and food samples, isolation of plasmid, genomic DNA, Isolation of metagenomic DNA from various sources (food, water, soil, sewage etc.), Identification of AMR microorganisms, identification and characterization of antibiotic resistance genes (ARGs), microbiological testing of nanoparticle samples etc.
Microbial Repository: pure, characterized AMR/ non-AMR microbial strains can be provided on a charged basis.
Outsourcing services: for various government & private agencies, for AMR based research work, short-term projects, analysis services on genomic and metagenomic samples, data collection, collation, database generation etc.
Consultancy Services: for various regulatory bodies/ private agencies etc.
Training: aimed at capacity building, skill development, internship, mentoring etc. for students, researchers, teachers, in-service & pre-service personnel (for government, public/private organizations and industries)
Courses: diploma/ certificate/ short-term/ orientation & refresher programs and hand-on workshops on AMR can be carried out.
Research Funding: Major research grant proposal writing for National /international government/ private agencies.
Scientific Social Responsibility (SSR): free community outreach for school/college students or society is carried out for training and community sensitization & awareness about AMR, Health and Hygiene.
Techniques routinely carried out in the AMR Lab
Microbiology:
Antibiotic Susceptibility Test (AST) as per CLSI and EUCAST guidelines
Determination of MIC and FIC of antimicrobial agents (antibiotics, nanoparticles, organic solvents and other materials) as per CLSI and EUCAST guidelines
Microbial Growth curve experiments with/without stressors
Cytotoxicity studies on bacteria (using different dyes like MTT)
Biofilm formation and inhibition experiments on microtiter plates
Isolation, purification and identification of microbial strains from any sample.
Maintenance of microbial strains on culture plates and slants.
Molecular Biology:
Various formats of Polymerase chain reactions (PCR)
DNA isolation (plasmid and genomic)
Metagenomic DNA isolation
Agarose Gel Electrophoresis & purification of samples from gels
Polyacrylamide gel electrophoresis (native and SDS) and subsequent analysis of gel
Bioinformatics:
Primer Designing
Amplification and 16S rRNA sequence
Identification of microorganism using 16S rRNA sequence (BLAST)
Analysis of metagenomic DNA sequence data (16S or WGS)
Biochemistry:
Measurement of total DNA/ RNA/protein concentration of sample
Sample purity checking (plasmid/genomic DNA/proteins spectrophotometrically/gel based)
Measurement of total sugar/ carbohydrate concentration of sample (DNSA method)
Spectrophotometric studies of samples (UV-Visible/ fluorescent/ luminescent), cuvette and microtitre well formats
Materials Science/Bio-Nanotechnology:
Method standardization & Synthesis of metal nanoparticles (silver, zinc oxide etc.)
Stability testing of nanoparticles (measuring UV-Vis peak)
Characterization of nanoparticles w.r.t. size, shape etc.
Major Equipment available with the AMR Lab