Department of Zoology

Target Audience: Undergraduate students with basic knowledge of Zoology, Genetics and Molecular Biology

Description/objective:

• Week 1: Animal Handling & Tissue Culture (4 hours)

Introduction to animal handling, handling of Drosophila culture (as model system); Introduction to mammalian cell culture (WBC as model).

• Week 2: DNA Isolation & Quantitation (4 hours)

Isolation of DNA from Drosophila, quantitation by spectrophotometry/ biochemical assay, Agarose gel electrophoresis.

• Week 3: Protein Isolation & Quantitation (4 hours)

Isolation of Protein from Drosophila, quantitation by spectrophotometry/biochemical assay, Polyacrylamide gel electrophoresis.

• Week 4: ELISA (4 hours)

Analysis of human blood samples for disease identification.

• Week 5: Histological Methods (4 hours)

Sectioning of organ tissue using microtome, and Hematoxylin/Eosin staining of tissue.

• Week 6: PCR, RAPD, RFLP (4 hours)

Basic procedures to identify different diseases.

• Week 7: Microbiological Techniques (4 hours)

Basic microbiological techniques including sterilization, inoculation, isolation, culture and incubation methods of bacteria.

• Week 8: Evaluation

Test of 30 marks (Objective type and analytical) [1 hour] and Seminar Presentation with interactive session [1 hour] will be organized.

Learning Outcomes:

After completion of the course, the student will be able to

(i) explain the molecular aspects of human diseases (Cognitive level*: understand)

(ii) explain the role of molecular markers to identify different diseases (Cognitive level: understand)

(iii) explain the role of signaling molecule during diseases (Cognitive level: understand)

(iv) identify the molecular tools to dissect out any particular disease (Cognitive level: apply)

(v) manage and care model animals used for scientific research (Cognitive level: apply)

(vi) manage cell and tissue culture for scientific research (Cognitive level: apply)

(vii) analyze the molecular data for the development of the disease per se (Cognitive level: analyze)

*Cognitive levels are as per the revised Bloom’s Taxonomy