During my first internship in Penn State University, I was mentored by Dr. Dudley in the research called “Characterization of Ethiopian and Pennsylvanian E. coli isolates”. These samples were obtained from a children’s hospital in Pennsylvania and from a farm in Ethiopia. In this research, we examined E. coli isolates and learned how to use the MiSeq machine. Once the data was uploaded to the website, we looked for the strains’ genes. Our focus were the genes that caused resistance to antibiotics. We came to a conclusion that the Pennsylvanian isolates had antibiotic resistance and the Ethiopian isolates didn’t. The Pennsylvanian ones caused diseases while the Ethiopian ones didn’t. In this research I learned a lot in the area of Microbiology.
Mentor: Dr. Dudley
During my second internship in Penn State University, I was continue being mentored by Dr. Dudley in the research titled "Young claves, missing bacteriophages: A fascinating phage isolation revelation. In this project we intended to isolate bacteriophages from Holstein cows' feces, characterizes those isolated bacteriophages using transmission electron microscope (TEM) and determine the capacity of infection they have in wild-type Escherichia coli. We collected thirty bacteriophages from cows that are at least one month old. They show different infection patterns, even when they were fromo the same family.
Mentor: Dr. Dudley
Charliz presenting the results of their research!!