Embedded Files
SPRING SEMESTER - RESEARCH
Dr. Herberholz lab
Week 1 (2/3-2/7)
Met with Dr. Herberholz and discussed what I will be doing
Learned lab set-up and how to care for the crayfish
Learned basic lab techniques such as how to cannulate (insert tube into the heart)
Isolated 4 crayfish (2 male 2 female) into small tanks so that next week they will be ready to experiment on
Week 2 (2/10-2/14)
Injected strong dose of picrotoxin (50 micromolar) into a communal crayfishÂ
3 doses spread out over an hour, 20 mins apartÂ
Injected very weak dose of picrotoxin (7.5 micromolar) into isolated crayfish and then placed into alcohol for an hour
Isolated 4 more crayfish (2 male 2 female) to be ready to use next weekÂ
Week 3 (2/17-2/21)
Injected even stronger dose than last week of picrotoxin (100 micromolar) into a communal crayfishÂ
3 doses spread out over an hour, 20 mins apartÂ
Same procedure as last week
Isolated 1 female crayfish
Experimented on 1 female isolate and 1 male isolate using the picrotoxin and alcohol procedure
Week 4 (2/24-2/28)Â Â Â
experimented on 1 male communal with picrotoxin and alcohol
got to rest for about 5 hours after cannulation (4 hours more than normal)
experimented on 1 female communal with picrotoxin and alcohol
blood clot formed and made pushing picrotoxin very difficult, had to manipulate cannula, wiggle it, and draw back in order to be able to inject picrotoxin
experimented on female isolate with picrotoxin and alcohol
found a small patch of mold in the 7.5 uM picrotoxin solution, bleached the bottle and made a new mixture before experiments
analyzed videos from 2/11 and 2/14
Week 5 (3/2-3/6)
experimented on 1 male communal with picrotoxin and alcohol
animal died following experiment
experimented on 1 male communal with 100 um picrotoxin only, no alcohol
cannula came out after first picrotoxin injection, so only 1 mL injected rather than 3
analyzed videos from 2/14, 2/21, 2/18, and 2/25
Week 6 (3/9-3/13)
analyzed videos from 2/28, and 3/6
began compiling data and notes and uploaded to the Box
Week 7
Spring Break
Week 8
reviewed research from my PI
Neuro cellular effects of alcohol are not well understood becuase it works on many different receptors in the brain at once
affects serotonin and GABAÂ
many studies show how alcohol affects social behaviors but very few look at how social experiences affect neurobiology of alcohol
isolated crayfish spontaneously tail flipped later than communal crayfish
the potentiation threshold for the tail flip neuron was significantly lowered by alcohol exposure
this shows that prior social experience has significant effects on a single cellular level
Week 9
Compiled all data in excel
Â
FALL SEMESTER - SEMINAR
HW2
My shell is bash
I am in the cms-opendata directory
"ls" displays the directory and files in orderÂ
"ls -l" just uses a longer listing format
"ls -l" and "ls -l -h" seem to be almost identical except when -h is added it simply turns the 4096 and total 12 into 4K and 12K
"ls -h" doesn’t provide all of the info that "ls -l" does, it just keeps it simplified to the directory and file
"mkdir" creates a new folder, or directory, which we named new_directory. ls then confirmed that the new directory had been created by showing it as an option.Â
"cd" changed the directory we were in to the new_directory that was just created.
"pwd" command means to print working directory, or show the path taken to get to the current directory you are inÂ
the “..” command is similar to a back button, and indicates the previous location
"rmdir" is used to remove or delete a directory, so when the "ls" command is given to list the folders and files, new_directory is now gone
using the up arrow enters previous commands from lines above, so that you don’t have to type them out again
these seem to display similar information but in an inverse order
Hitting tab filled changed cms to cms- opendata/ and then allowed me to see the directories and files listed out
"whoami" tells you what home directory you are using, for example even when I switched into the downloads directory the whoami command still said cms -opendata
The ~ can be used as a symbol for your home directory instead of having to write out the whole name
"." is used to denote the current directory and ".." means one level back/above, so "cd.." is similar to a back button
File Operations (for HW)
Emacs
HW3
This is the result of running the script with "cms" as the argument. This seems to search for all files with cms in the title and display their pathÂ
"chmod" changes the permission of what you are able to do with a file, so "chmod +x" makes it an executable file
HW4
the "++" operator adds one to the variable while the "--" operator subtracts one
HW5
HW6
HW7
HW8
Page updated
Report abuse