Subtract uniform background

Subtract uniform background

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Description:

1. This macro subtracts the mean background fluorescence signal from each slice of a time-lapse stack.

2. User needs to draw an ROI representing the background area and make sure that the ROI does not pass through the real signal (i.e. a cell) in any of the slices.

3. The output is a new file with a name = original stack name + _bs