Pipette assay macro

Pipette assay macro

In a pipette assay, chemoattractant (e.g. EGF) is delivered through a pipette and the cell responds by extending a protrusion in the direction of the pipette. This macro (available here) was written to analyze the response of the cell (i.e. protrusion or retraction) along 16 radial directions (22.5 degrees apart) originating from the cell centroid to the cell periphery, where angle 0 degrees corresponds to the line joining the cell centroid to the pipette tip. The macro was successfully used in the following article:

Bravo-Cordero JJ, Sharma VP, Roh-Johnson M, Chen X, Eddy R, Condeelis J, Hodgson L. Spatial regulation of RhoC activity defines protrusion formation in migrating cells. J Cell Sci. 2013 Aug 1;126(Pt 15):3356-69

A flow diagram of how to use the macro is described below.