Outline leading edge segment

Outline leading edge segment

This macro (available here) can be used to draw a segment ROI of user-specified thickness at the leading edge of a fluorescent cell, which can then be used to measure, e.g. mean fluorescence intensity in that segment. The macro was successfully used in the following article:

Bravo-Cordero JJ, Sharma VP, Roh-Johnson M, Chen X, Eddy R, Condeelis J, Hodgson L. Spatial regulation of RhoC activity defines protrusion formation in migrating cells. J Cell Sci. 2013 Aug 1;126(Pt 15):3356-69

A flow diagram of how to use the macro is described below.