RT-PCR Using NOVASCRIPT-III-SS Kit

Reaction Mixture:

2X One-Step RT-PCR Buffer 25.0µl

One Step Enzyme Mix 2.0µl

Forward Primer (50µl) 0.2µl

Reverse Primer (50µl) 0.2µl

RNA Sample (500ng) 1.0µl

RNase Inhibitor(10 Units) 1.0µl

DEPC-Treated Water 20.6µl

PCR-Program:

1. T= 42 30min

2. T= 95 10min

3. T= 95 30sec

4. T= 55 30sec

5. T= 72 1:30min (Depending on Resulting Amplicon Size, 1min for 1kb DNA)

6. GO TO 3 REP 35

7. T= 72 7min

8. HOLD 4