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Nucleic Acids and Protein Calculations
Metric Prefixes
Spectrophotometric Conversions
1 A260 unit of double-stranded DNA=50 µg/ml
1 A260 unit of single-stranded DNA=33 µg/ml
1 A260 unit of single-stranded RNA=40 µg/ml
DNA Molar Conversions
Formulas for DNA Molar Conversions
Protein Molar Conversions
Protein/DNA Conversions
Agarose Gel(%):Resolution of Linear DNA
Polyacrylamide Gel(%):Resolution of Protein
Length/M.W. of Common Nucleic Acids.
Standards
1. Average MW of dsDNA base pair = 600.
2. Average MW of ssDNA base =330.
3. Average MW of RNA base =340.
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