The Advanced Flow Cytometry Platform offer users two identical Beckman Coulter "CytoFLEX" LX cell analysers. Having two instruments allows for equipment redundancy in robust immune monitoring assays
These are high end analytical flow cytometers with 5 lasers and up to 21 fluorescent parameters.
This system is our entry-level machine, and features in our Basic Training.
The Beckman Coulter CytoFLEX LX analysers are high end analytical flow cytometers with 5 lasers and up to 21 fluorescent parameters. They are the tools of choice for high end polychromatic flow cytometry where improved compensation and better quantification of fluorescence measurements are essential. The instruments feature avalanche photdiodes (APD) for exceptional linearity. Specifications:
Lasers (5): 355 nm (UV), 405 nm (violet), 488nm (blue), 561nm (yellow-green), 633 nm (red).
Detectors (20 parameters): 3 Blue/3 Red/5 Violet /4 UV/ 5 Yellow-Green.
Both of our CytoFLEX instruments have tube and built-in plate loader options. CytExpert software is used for acquisition and analysis.
The analysers are available self-service only, 24 hours a day, 7 days a week for use by investigators except during weekly maintenance and repair periods.
You can book on the Equipment pages of our iLab calendar.
Before an investigator can book and use the equipment, they must register with the Flow Core and attend mandatory Basic Training. Existing users who had training prior to 2022 may only have had FACSCanto training, in which case a free CytoFLEX top-up training is available (provided they remained regular users of the Flow Core).
IMPORTANT Compared with the Fortessa there is one less channel on the violet laser (BV570/BV650 is not supported). There are extra channels on the UV and yellow-green lasers. The PerCPCy5.5 channel detects all PerCP derivatives (PerCP, PerCP-Cy5.5, PerCP-Vio700, BB700).
Filter swaps: unlike the Fortessas, we do not permit or recommend users swap the detector filters on the CytoFLEX systems.