Ludwig PE, Reddy V, Varacallo MA. Neuroanatomy, Neurons. [Updated 2023 Jul 24]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK441977/
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Ismaeil, Rehab & Hui, Chua & Ahmad Affandi, Khairunisa & Alallam, Batoul & Mohamed, Wael & Noor, Mohd. (2021). Neuroprotective effect of edible bird’s nest in chronic cerebral hypoperfusion induced neurodegeneration in rats. Neuroimmunology and Neuroinflammation. 2020. 10.20517/2347-8659.2020.63.
Primarily used as a neuronal stain, serving as a crucial part of the Nissl stain (named after German scientist Franz Nissl). The way cresyl violet stains neurons is by staining nissl bodies in particular. Nissl bodies are organelles located within neurons responsible for protein synthesis and the distribution of gray matter. In this way, by staining nissl bodies, you effectively stain the vast majority of a neuron.
There are no indications of any hazards associated with the use of cressyl violet. However, reasonable caution is still strongly recommended.