Histology is the study of tissue structure at a microscopic level, connecting tissue structure with its function.
Histology almost always uses dyes and stains to color tissues before examining them under the microscope. Without staining a tissue sample, structures tend to be difficult or impossible to see. Further, stains and dyes allow scientists to highlight specific key structures, with certain dyes targeting specific molecules or organelles.
During your time at Mount Holyoke, you may find yourself in a position where you want to use histological stains in your research. Follow this page to learn about the history of these dyes and to guide you towards the dye best suited to your sample.
Roetzer-Pejrimovsky, T., Moser, AC., Atli, B. et al. The Digital Brain Tumour Atlas, an open histopathology resource. Sci Data 9, 55 (2022). https://doi.org/10.1038/s41597-022-01157-0
(2023, October 26). histology. Encyclopedia Britannica. https://www.britannica.com/science/histology
When using any lab material (including these stains) you should be at least wearing long sleeves, closed-toed shoes, and nitrile gloves. When handling certain materials you may want to add on safety goggles or perform your task under a ventilated hood. Make sure that before you use any chemical you look up its lab safety data sheet (SDS). This will tell you any hazards associated with the chemical and what you should do if it comes in contact with you.
PubChem has good information on chemicals and lists their hazards
Sigma Aldrich has downloadable safety data sheets
Wikipedia can be a good way to find general information (like boiling point)
Confused about histology vocabulary you see being used online? Check out the Biological Stain Commission's online glossary!
[still under construction]
[still under construction]
Interested in learning about the fascinating history of biological stains? Check out this webpage!