One of the key tools scientists use to study how traits are passed down from parents to offspring is a PUNNETT SQUARE. Punnett squares are useful for predicting the probability that the offspring of a pairing will have a certain genotype and phenotype.
A Punnett square is a model that helps you find the probability that an offspring will have a genotype or phenotype. But the probabilities you find with a Punnett square are not guaranteed to happen in real life. Probability is just a predictor of the chances that an event will happen; it does not definitively state that it will actually occur.
To illustrate how a Punnett square works, let's look at the experiments done by "The Father of Genetics" Gregor Mendel's work with pea plants.
The pea plants Mendel studied had two alleles for height: a dominant tall allele (T) and a recessive short allele (t). Since pea plants produce using sexual reproduction each pea plant puts one of its two alleles for height into every gamete that it makes. Each allele for height has an equal chance of becoming part of a gamete, so half of the gametes carry one allele, and half of the gametes carry the other allele.
In a Punnett square, the alleles of the parents are placed on the top and side of the square. So if one plant is homozygous dominant for height (TT), and the other plant is homozygous recessive for height (tt) we would arrange these two PARENT/P GENERATION plants around the outside of the Punnett square. And to find the possible genotypes made from this pairing you would fill the inside of the square with the corresponding allele from each parent.
The offspring of the P-generation are called the FIRST FILIAL/F1 GENERATION. And the offspring of two F1-generation parents is known as the SECOND FILIAL/F2 GENERATION.
We will be focusing on two major types of crosses: MONOHYBRID CROSSES that involve one trait and DIHYBRID CROSSES that involve two traits.
How to Solve Genetic Problems:
Make a key, by assigning each trait a letter. (ALWAYS WRITE DOWN YOUR KEY!)
Dominant Trait = Capital Letter (Ex: D)
Recessive Trait = lowercase letter (Ex: d)
Determine the Genotypes of the Parents.
Pure = Homozygous dominant (DD) or Homozygous Recessive (dd)
Hybrid/Mixed/Carrier = Heterozygous (Ex: Dd)
Fill out the possible offspring genotypes in the Punnett Square!
Remember to place the gametes or alleles from each parent on the tops and sides
Answer the question(s).
How to solve a monohybrid Punnet Square
How to Solve Genetic Problems:
Make a key, by assigning each trait a letter. (ALWAYS WRITE DOWN YOUR KEY!)
Dominant Trait = Capital Letter (Ex: D)
Recessive Trait = lowercase letter (Ex: d)
Determine the Genotypes of the Parents.
Pure = Homozygous dominant (DD) or Homozygous Recessive (dd)
Hybrid/Mixed/Carrier = Heterozygous (Ex: Dd)
Fill out the possible offspring genotypes in the Punnett Square!
Remember to place the gametes or alleles from each parent on the tops and sides
Answer the question(s).
How do you find the parent's gametes in a dihybrid cross?
F.O.I.L. = First, Outer, Inner, Last