002

Dynamic exchange of abundant scaffold-bound and rare freely diffusing ligands forms Wnt gradient.

Yusuke Mii^1,2,3, Kenichi Nakazato⁴, Chan-Gi Pack^6,7, Takafumi Ikeda³, Yasushi Sako⁶, Atsushi Mochizuki^4,5, Masanori Taira^3,8, Shinji Takada¹

¹ National Institute for Basic Biology and Exploratory Research Center on Life and Living Systems (ExCELLS)

² JST, PRESTO

³ Graduate School of Science, The University of Tokyo

⁴ Theoretical Biology Laboratory, RIKEN

⁵ Institute for Frontier Life and Medical Sciences, Kyoto University

⁶ Cellular Informatics Laboratory, RIKEN

⁷ ASAN Institute for Life Sciences

⁸ Faculty of Science and Engineering, Chuo University

005

ERK-mediated Curvature Feedback in Branching Morphogenesis of Lung Epithelial Tissue

Tsuyoshi Hirashima ^{1, 2, 3}, Michiyuki Matsuda^{2, 4}

¹ The Hakubi Center, Kyoto University

² Graduate School of Biostudies, Kyoto University

³ Japan Science and Technology Agency, PRESTO

⁴ Graduate School of Medicine, Kyoto University

006

Endothelial Cell Signature and Potential of Mouse Skeletal Muscle Stem Cell Revealed by Single Cell RNA-seq

Mayank Verma^1,2,3, Yoko Asakura^2,3 , Xuerui Wang^2,3, Kasey Zhou^2,3, Mahmut Ünverdi^2,3, Allison P. Kann⁴, Robert S. Kraus⁴ and Atsushi Asakura^2,3

¹ Department of Pediatrics & Neurology, Division of Pediatric Neurology, The University of Texas Southwestern Medical Center

² Stem Cell Institute, University of Minnesota Medical School

³ Paul & Sheila Wellstone Muscular Dystrophy Center, University of Minnesota Medical School

⁴ Department of Cell, Developmental, and Regenerative Biology, Icahn School of Medicine at Mount Sinai

007

Exposure of light affects the mouth form dimorphism in the nematode Pristionchus pacificus

Chinatsu Kai ¹, Takahiro Chihara ^1,2, Misako Okumura ^1,2

¹ Department of Biological Science, School of Science, Hiroshima University

² Graduate School of Integrated Sciences for Life, Hiroshima University

012

Sall1/4 are required for cell proliferation of cardiac progenitors and cardiomyocytes

Wataru Katano¹, Shunta Mori ², Yuki Tajika², Jun K. Takeuchi³, and Kazuko Koshiba-Takeuchi ⁴

¹ Graduate School of Life Sciences, Toyo University

² Faculty of Life Sciences, Toyo University

³ Graduate School of Medicine, Gunma University

⁴ Medical Research Institute, Tokyo Medical and Dental University

020

Twist and elongation of gut tube independently arize through chiral cell sliding and convergent extension, respectively, in epithelial tissue

Mikiko Inaki, and Kenji Matsuno

Department of Biological Science, Graduate School of Science, Osaka University

024

Injury-induced TGF-β1 signaling is essential for tissue regeneration in the Xenopus tadpole tail

Makoto Nakamura ¹, Hitoshi Yoshida ², Yuka Moriyama ¹, Itsuki Kawakita ¹,

Marcin Wlizla ², Kimiko Takebayashi-Suzuki ¹, Marko E. Horb ² and Atsushi Suzuki ¹

¹ Graduate School of Integrated Sciences for Life, Hiroshima University, Japan

² National Xenopus Resource and Eugene Bell Center for Regenerative Biology and Tissue Engineering, Marine Biological Laboratory, USA

026

Dual-color live imaging reveals dynamic interplay between eRNA transcription and target gene expression in Drosophila embryos

Kota Hamamoto ^{1, 2} and Takashi Fukaya ^{1, 2}

¹ Institute for Quantitative Biosciences, University of Tokyo

² Graduate School of Arts and Sciences, University of Tokyo

027

Changes in the migration behavior of zebrafish neural crest cells under low O₂ conditions.

Ayaka Kasamatsu¹, Hidenori Hashimura², and Satoshi Sawai^1,2

¹ Department of Biological Sciences, Graduate School of Science, The University of Tokyo

² Graduate School of Arts and Sciences, the University of Tokyo

028

Maternal expression of dazl contributes to the eary stage of PGC differentiation in the urodele amphibian

Mitsuki Kyakuno ^1,2, Tetsushi Sakuma ¹, Ken-ichi Suzuki ^1,3, Takashi Yamamoto ¹, Ichiro Tazawa ^1,2, Nobuaki Furuno ^1,2, Toshiaki Noce ⁴, Naoki Tsunekawa ⁵, Takashi Takeuchi ⁶, and Toshinori Hayashi ^1,2

¹ Graduate School of Integrated Sciences for Life, Hiroshima University

² Amphibian Research Center, Hiroshima University

³ Center for the Development of New Model Organisms, NIBB

⁴ Faculty of Medicine, Keio University

⁵ College of Bioresource Science, Nihon University

⁶ School of Life Science, Tottori University

029

Spatio-temporal regulation of Msx1 in the vertebrate jaw primordium

Yoshio Wakamatsu ¹, Hikaru Kon ¹, and Kunihiro Suzuki ²

¹ Department of Developmental Neuroscience, Graduate School of Medicine, Tohoku University

² School of Dentistry at Matsudo, Nihon University

031

Major function of the cement gland of Xenopus larvae is to stop the reflective movements induced by both of the shadow or touch stimuli before swimming stages

Yumiko Harada¹, Megumi Takeyasu², Satomi Seii², Ayaka Ando², and Ryutaro Murakami¹

¹ Grad.Sch.Sci.Tech. for Innov., Yamaguchi Univ.,

² Dept.Biol Sci. Chem., Fac. Sci., Yamaguchi Univ.

032

Overlapping signal gradient of EGFR and DPP determine the position of dorsal appendages of the Drosophila egg

Ryutaro Murakami^{1, 2, 3}, Tsubasa Saitoh ², Yushi Matsuwaka ³, Yumiko Harada ¹

¹Grad Sch Sci Tec for Innov, Yamaguchi Univ

²Grad Sch Med, Yamaguchi Univ.

³Dept Biol, Yamaguchi Univ, Yamaguchi 753-8512, JAPAN

034

Regulation of androgen-dependent sexual character development in Japanese medaka: Combinatorial analyses of RNA-seq and ATAC-seq

Yukiko Ogino ¹, Satoshi Ansai ², and Taisen Iguchi ³,

¹ Faculty of Agriculture, Kyushu University

² Graduate School of Life Sciences, Tohoku University

³ Graduate School of Nanobioscience, Yokohama City University

035

Akaluc/AkaLumine bioluminescence system enables highly sensitive, non-invasive, and temporal monitoring of gene expression in Drosophila

Akira Ito ¹, Nagisa Matsuda ¹, Misako Okumura ¹, Takahiro Chihara ¹

¹ Program of Biomedical Science, Graduate School of Integrated Sciences for Life, Hiroshima University

036

Deletion of CTCF sites in the SHH locus alters enhancer–promoter interactions and leads to acheiropodia

Aki Ushiki ^1,2, Yichi Zhang ^1,2, Chenling Xiong ^1,2, Jingjing Zhao ^1,2,

Ilias Georgakopoulos-Soares ^1,2, Lauren Kane ³, Kirsty Jamieson ², Michael J. Bamshad ⁴, Deborah A. Nickerson ⁴, Yin Shen ^2,5, Laura A. Lettice ³,

Elizabeth Lemos Silveira-Lucas ⁶, Florence Petit ⁷, Nadav Ahituv ^1,2

¹ Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, USA

² Institute for Human Genetics, University of California San Francisco, USA

³ MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, UK

⁴ Department of Genome Sciences, University of Washington, USA

⁵ Department of Neurology, University of California San Francisco, USA

⁶ Consultorio Genetica Clinica, Brazil

⁷ CHU Lille, University of Lille, France

037

Modeling limb skeletogenesis in 3D culture derived from mouse embryonic limb bud and human ES cells

Rio Tsutsumi¹, Mototsugu Eiraku^1,2

¹ Institute for Advanced Study of Human Biology (WPI-ASHBi), Kyoto University

² Institute for Frontier Life and Medical Sciences, Kyoto University

038

RAR agonist antagonizes zebrafish fin regeneration

Kumpei Murase, Eri Tanishita, Tomoya Nakashima, Ayumi Nagashima, Atsushi Kawakami

Department of Life Science and Technology, Tokyo Institute of Technology

039

Regeneration mechanism of size and morphology in the zebrafish

Misato Yokozawa, Keina Matsumura, Yuuki Yokota, Atsushi Kawakami

Department of Life Science and Technology, Tokyo Institute of Technology

040

Mechanical role of Reichert’s membrane for early mouse morphogenesis in utero.

Yoko Ueda ¹, Chiharu Kimura-Yoshida ¹, Kyoko Mochida ¹, Mami Tsume ¹, Ryuji Hiramatsu ², and Isao Matsuo ¹

¹ Department of Molecular Embryology, Research Institute, Osaka Women’s and Children’s Hospital, Osaka Prefectural Hospital Organization

² Department of Veterinary Anatomy, The University of Tokyo

041

Reconstruction of 3D structures from serially sectioned histological slides after automated ROI detection and fine alignement

Sanae Oka¹, Ayumi Hamano², Timothy Day¹, Yoko Higuchi¹, Dongbo Shi¹, Jun Sakurai^1,3, Seiichi Uchida² and Toshihiko Fujimori^1,3

¹ Division of Embryology, National Institute for Basic Biology

² Department of Advanced Information Technologies, Kyushu University

³ Department of Basic Biology, School of Life Science, SOKENDAI, The Graduate University for Advanced Studies

042

Identification of an inhibitor against Notch signaling secreted from Drosophila hemocytes carrying pecanex mutation.

Rin Fujii¹, Tomoko Yamakawa², Kenji Matsuno³

¹ Department of Biological Science, Osaka University

050

Identification and characterization of Hiat, a novel Hippo pathway-interacting amino acid transporter

Daichi Honda¹, Misako Okumura¹, Tomoki Umehara², Chisako Sakuma³, Toshiyuki Fujii¹, Masayuki Miura² and Takahiro Chihara¹

¹Program of Biomedical Science, Graduate School of Integrated Sciences for Life, Hiroshima University. ²Graduate School of Pharmaceutical Sciences, The University of Tokyo. ³Department of Tropical Medicine, The Jikei University School of Medicine.

051

Hypothetical mechanism for variegated body pigmentation: epigenetic alteration of local PKA activity

Makoto Goda ¹, Yuko Wakamatsu ², Kenichi Kobayashi ³, Chikako Inoue ², Myra Villareal ⁴, Mariko Kikuchi ², Hiroko Isoda ⁴, Katsumi Ohtani ³, Toshiyuki Nishimaki ⁵, Hiroki Oota ⁵, Motoyuki Ogawa ⁵, Tetsuaki Kimura ⁶, Kiyoshi Naruse ⁶, Minoru Tanaka ², Robert N. Kelsh ⁷, Masahiko Hibi ², Hisashi Hashimoto ²

¹ Hamamatsu University School of Medicine, ² Graduate School of Science, Nagoya University, ³ National Institute of Occupational Safety and Health, Japan, ⁴ Faculty of Life and Environmental Sciences, Tsukuba University, ⁵ Kitasato University School of Medicine, ⁶ National Institute for Basic Biology, ⁷ University of Bath

052

Auxin-inducible protein knockdown system established in mice

Yumiko Saga ^1,2, Hatsune Ito ¹, Akemi Okubo ¹, Rieko Ajima ^1,2, Masato Kanemaki ^2,3

¹ Department of Gene Function and Phenomics, National Institute of Genetics, ROIS.

² Department of Genetics, SOKENDAI.

³ Department of Chromosome Science, National Institute of Genetics, ROIS.

053

EGFR-dependent feedback mechanism protects epithelia from mechanical instability in Drosophila embryos

Kentaro Yoshida ^1,2 and Shigeo Hayashi ^1,2

¹ RIKEN Center for Biosystems Dynamics Research

² Department of Biology, Kobe University Graduate School of Science

054

Nano-patterning of insect cuticles by self-organization of apical ECM molecules

Yuki Itakura ¹, Zhengkuan Sun ^1,2, Sachi Inagaki ¹, Housei Wada ¹, and Shigeo Hayashi ^1,2

¹ Center for Biosystems Dynamics Research, RIKEN

² Department of Biology, Kobe University

056

Visualization and quantitative analysis of endogenous Wnt8 protein in zebrafish

Taijiro Yabe¹², Yusuke Mii¹²³, Shinji Takada¹²

¹ Department of Division of Molecular and Developmental Biology, National Institute for Basic Biology

² Developmental Signaling Research Group, Exploratory Research Center on Life and Living Systems (ExCELLS)

³ PRESTO, JST

057

A novel molecular mechanism regulating chondrocyte cell polarity that is essential for endochondral ossification.

Koji Kikuchi¹, Mami Nakagawa², Toshihiko Fujimori², Kei-ichiro Ishiguro³, Kimi Araki⁴, Akira Nakamura⁵, Makoto Suzuki⁶, and Hiroyuki Nakanishi¹

¹ Department of Molecular Pharmacology., Graduate School of Medical Sciences, Kumamoto University

² Division of Embryology, National Institute for Basic Biology

³ Department of Chromosome Biology, Institute of Molecular Embryology and Genetics, Kumamoto University

⁴ Institute of Resource Development and Analysis, Kumamoto University

⁵ Department of Germline Development, Institute of Molecular Embryology and Genetics, Kumamoto University

⁶ Department of Pharmacology, Jichi Medical University

059

Mechanisms Underlying Left-Right Asymmetry Formation of the Drosophila Brain

So Sakamura¹, Fuyu Hsu ², Ann-shyn Chiang ², and Kenji Matsuno ¹

¹ Department of Biological Sciences, Graduate School of Science, Osaka University

² Institute of Biotechnology , National Tsing Hua University, Taiwan

060

The coordination of meiotic gene activation and pre-meiotic S phase entry ensure the reproductive life span in murine female

Ryuki Shimada ¹ and Kei-ichiro Ishiguro ¹

¹ Department of Chromosome Biology, IMEG, Kumamoto University

063

Mathematical models of cell sorting: micro- and macroscopic perspectives

Hideki Murakawa ¹

¹ Faculty of Advanced Science and Technology, Ryukoku University

067

Activities and morphology of individual cells derived from three germ layers associated with the region-specific movements of the larval zebrafish gut

Daiji Takamido ¹, Shin-ichi Okamoto ¹, Koudai Shimomura ², Koichi Kawakami ³, Masataka Nikaido ⁴, and Kohei Hatta ⁴

¹ Graduate School of Life Science, University of Hyogo

² Department of Science, University of Hyogo

³ National Institute of Genetics

⁴ Graduate School of Science, University of Hyogo

068

Single cell transcriptome analysis of basal chordate Branchiostoma japonicum

Hitoshi Tominaga ¹, Koki Nishitsuji ¹, Masato Kiyomoto ², Kanako Hisata ¹, Jun Inoue ³, and Noriyuki Satoh ¹

¹ Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University

² Tateyama Marine Laboratory, Marine and Coastal Research Center, Ochanomizu University

³ Atmosphere and Ocean Research Institute, University of Tokyo,

070

Cell competition effector Sas-Ptp10D facilitates apoptosis for the proper shaping of germ-line stem cell niche

Kiichiro Taniguchi ¹ and Tatsushi Igaki ¹

¹ Laboratory of Genetics, Graduate School of Biostudies, Kyoto University

074

Scleraxis-lineage cells are required for correct muscle patterning

Yudai Ono¹, Tempei Sato^2,3, Chisa Shukunami⁴, Hiroshi Asahara³, Masafumi Inui ^1,2

¹ Laboratory of Animal Regeneration Systemology, Department of Life Science, School of Agriculture, Meiji University

² Department of Systems BioMedicine, National Research Institute for Child Health and Development, Tokyo 157-8535, Japan

³ Department of Systems BioMedicine, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo 113-8510, Japan

⁴ Department of Molecular Biology and Biochemistry, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, 734-8553, Japan.

078

Possible involvement of the cellular localization of Ouro1 and Ouro2 proteins in the tadpole tail cell death of Xenopus laevis

Haruka Kobayashi ¹, Yumi Izutsu ²

¹ Graduate School of Science and Technology, Niigata University

² Faculty of Science, Niigata University

079

Investigation of the role of germ cells in the regulation of organismal aging using a shortest-lived vertebrate model

Kota Abe ¹ and Tohru Ishitani ¹

¹ Division of Cellular and Molecular Biology, Department of Homeostatic regulation, Research Institute for Microbial Diseases, Osaka University

080

FGF controls axis rotation and concave-to-convex conversion of columnar cells during spheroidal eye lens formation

Yuki Sugiyama and Ichiro Masai

Developmental Neurobiology Unit, Okinawa Institute of Science and Technology Graduate University (OIST)

083

Water permeation into the vacuoles facilitates cell rounding and release during endothelial-to-hematopoietic transition

Mugiho Shigematsu ¹, Sho Maejima ², Maria Shibata ¹, Chie Tamura ¹, Thomas M Schultheiss ³, Hirotaka Sakamoto ², and Yuki Sato ¹

¹ Graduate School of Medical Sciences, Kyushu University, Japan

² Ushimado Marine Institute, Graduate School of Natural Science and Technology, Okayama University, Japan

³ Department of Genetics and Developmental Biology, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Israel

084

Time-course individual RNA-Seq revealed a transcriptomic landscape of environmental sex determination sex determination in zebrafish

Makoto Kashima ¹, Kanako Nishimura ¹, Hirata Hiromi ¹

¹ College of Science and Engineering, Aoyama Gakuin University

085

Starvation induces precocious maturation in Drosophila melanogaster

Yuko Shimada-Niwa ^1,5, Hsin Kuang Lin ², Yuya Ohhara ³, Hassna Hanif ⁴, Kanato Abe ⁴, Naoki Okamoto ¹, Ryusuke Niwa ¹

¹ Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba

² Graduate School of Life and Environmental Sciences, University of Tsukuba

³ School of Food and Nutritional Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka

⁴ College of Biological Sciences, University of Tsukuba

⁵ PRESTO, JST

086

The interaction between nutrient and gut hormone in mating-induced germline stem cell proliferation in the fruit fly Drosophila melanogaster

Ryo Hoshino ¹, Yuto Yoshinari ¹, Shu Kondo ², Hiromu Tanimoto ³, Hiroko Sano ⁴, and Ryusuke Niwa ^5,6

¹ Faculty of Life and Environmental Sciences, University of Tsukuba

² Faculty of Advanced Engineering, Tokyo University of Science

³ Graduate School of Life Sciences, Tohoku University

⁴ Institute of Life Science, Kurume University

⁵ Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance, University of Tsukuba

⁶ AMED-CREST, AMED

089

Periodic accumulation of interstitial cells generates the branched architecture of Cladonema medusa tentacles

Shiting Hou¹, Jiangrong Zhu¹, Saki Shibata², Ayaki Nakamoto³ and Gaku Kumano¹

1. Asamushi Research Center for Marine Biology, Graduate School of Life Sciences, Tohoku University; 2. School of Veterinary Medicine, Kitasato University; 3. Department of Pharmacy, Aomori University

090

Force-dependent remodeling of a tight junction protein ZO-1 is regulated by phase separation

Noriyuki Kinoshita ^{1, 3}, Takamasa Yamamoto ¹, Naoko Yasue ¹, Chiyo Takagi ¹, Toshihiko Fujimori ^{2, 3}, and Naoto Ueno ^{1, 3}

¹ Division of Morphogenesis, Department of Developmental Biology, National Institute for Basic Biology

² Division of Embryogenesis, Department of Developmental Biology, National Institute for Basic Biology

³ School of Life Science, SOKENDAI (The Graduate University for Advanced Studies)

092

Glycolysis inhibits primordial germ-cell development of Drosophila melanogaster

Yoshiki Hayashi ^1,2, Ryo Kimura², Satoru Kobayashi^1,2

¹ Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba

² Graduate School of Science and Technology, University of Tsukuba

093

Single cell RNA-Seq to identify all cell types in the planarian Dugesia japonica.

Rei Komura ¹, Makoto Kashima ¹, Hiromi Hirata ¹

¹ College of Science and Engineering, Aoyama Gakuin University

094

Morphological changes of uterine epithelium during mouse implantation

Jun Sakurai ^1,2, Youko Higuchi ², Toshihiko Fujimori ,^1,2

¹ Department of Basic Biology, School of Life Science, SOKENDAI

² Division of Embryology, NIBB

095

Transcriptom analysis of Werner syndrome model in zebrafish

Kota Ujibe ¹, Makoto Kashima ¹, and Hiromi Hirata ¹

¹ Department of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University

096

Emergence of circumferential actin cables from clustered cross linked actin filaments during tubulogenesis

Sayaka Sekine ^{1, 2}, Mustafa M. Sami ¹, Housei Wada ¹ and Shigeo Hayashi ¹

¹ RIKEN Center for Biosystems Dynamics Research

² Graduate School of Life Sciences, Tohoku University

097

Feedback regulation among heparan sulfate proteoglycans, Wnt11, and core PCP components are involved in organization of planar cell polarity

Minako Suzuki ^{1, 2, 3}, Shinji Takada^{1, 2, 3}, Yusuke Mii ^{1, 2, 3, 4}

¹ Department of Basic Biology, The Graduate University for Advanced Studies, SOKENDAI

² National Institute for Basic Biology (NIBB)

³ Exploratory Research Center on Life and Living Systems (ExCELLS)

⁴ JST PRESTO

099

Heterogeneity of adult pluripotent stem cells revealed by single-cell analysis in planarian Dugesia japonica

Yoshihito Kuroki ^1,2 and Kiyokazu Agata ^1,2

¹ The Graduate University for Advanced Studies

² National Institute for Basic Biology

100

Physiological analysis of GABA_A receptor family in Zebrafish

Kenichiro Sadamitsu¹, Makoto Kashima¹, Hiromi Hirata¹

¹ Graduate School of Science and Engineering, Aoyama Gakuin University

101

The Drosophila PGC-1 homolog, Spargel, is required for germ granule assembly during oogenesis

Kazuko Hanyu-Nakamura ¹, Keisuke Aimi ^{2, 3}, Takuya Kurogi ², and Akira Nakamura ^{1, 2, 3}

¹ Institute of Molecular Embryology and Genetics, Kumamoto University

² School of Pharmacy, Kumamoto University

³ Graduate School of Pharmaceutical Sciences, Kumamoto University

105

Ultrastructural studies of nano-level ECM assembly during olfactory organ development in Drosophila

Sachi Inagaki¹, Takeshi Itabashi², Kenta Onoue³, Satoko Okayama³, Shigenobu Yonemura^3,4, Atsuko H. Iwane², Shigeo Hayashi¹

¹ Laboratory for Morphogenetic Signaling, RIKEN Center for Biosystems Dynamics Research

² Laboratory for Cell Field Structure, RIKEN Center for Biosystems Dynamics Research

³ Laboratory for Ultrastructural Research, RIKEN Center for Biosystems Dynamics Research

⁴ Department of Cell Biology, Tokushima University Graduate School of Biomedical Sciences

107

Roles of transcription regulators Skor1b/2 and Foxp1b/4 in differentiation of Purkinje cells in zebrafish

Tsubasa Itoh¹, Shinnosuke Yura¹, Akiko Nakanishi², Mari Uehara¹, Takashi Shimizu^1,2, and Masahiko Hibi^1,2

¹Grad. School of Science, Nagoya University, Nagoya, Japan

²Bioscience and Biotechnology Center, Nagoya University, Nagoya, Japan

109

Genomic and chromatin structure of narigoma enhancer that is required for left-right asymmetric development of embryonic gut.

Takuya Nomura ¹, Florian neugebauer ², Mikiko Inaki ³, Kenji Matsuno ⁴, and Oral Presenter ¹

¹ Department of Science, Osaka University

² Department of Science, Osaka University

³ Faculty of Science, Osaka University

⁴ Faculty of science, Osaka University

111

Analysis of Cajal Body Formation Mechanism

Saki Ohazama ¹, Hiroshi Maita ^1,2, Shinichi Nakagawa ^1,2

¹ Graduate School of Life Science, Hokkaido University

² Faculty of Pharmaceutical Sciences, Hokkaido University

113

Wnt-dependent mechanism of the apical constriction of roof-plate cells in the mouse spinal cord

Takuma Shinozuka ^1,2, and Shinji Takada ^1,2

¹ Division of Molecular and Developmental Biology, NIBB

² Developmental Signaling Research Group, ExCELLS

114

Left-right bias in the arrangements of the mother and daughter centrioles of the basal bodies of mouse node cilia.

Hiroshi Yoke ¹, Atsushi Taniguchi ¹ and Shigenori Nonaka ^1,2

¹ Laboratory for Spatiotemporal Regulations, National Institute for Basic Biology, National Institutes of Natural Sciences

² Department of Creative Research, The Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences

117

polished rice regulates Drosophila embryonic organogenesis in an ecdysone-dependent manner.

Yuki Taira¹, Housei Wada², Shigeo Hayashi² and Yuji Kageyama^{1, 3}

¹Biosignal Research Center, Kobe University

²RIKEN Center for Biosystems Dynamics Research

³Department of Biology, Graduate School of Science, Kobe University

120

Characterization of Exogenous Ribosome-Mediated Induced Somatic Cell Multipotency

Arif Istiaq^1,2,3,4, Mikiko Kudo ¹, Kousei Takashi ¹, Gu Haoxuan ¹, Tan Fuchao ¹, and Kunimasa Ohta¹

¹ Department of Stem Cell Biology, Kyushu University

² Graduate School of Medical Sciences, Kumamoto University

³ Institute of Molecular Embryology and Genetics, Kumamoto University

⁴ HIGO Program, Kumamoto University

122

The gene regulatory system for specifying germ layers in early ascidian embryos

Miki Tokuoka ¹, Kazuki Maeda ², Kenji Kobayashi ¹, Atsushi Mochizuki ³, and Yutaka Satou1 ¹

¹ Department of Zoology, Graduate School of Science, Kyoto University

² Faculty of Informatics, The University of Fukuchiyama

³ Institute for Frontier Life and Medical Sciences, Kyoto University

123

A catalytic activity-independent role for the extrinsic apoptotic pathway interacting with early endosomes to restrict axonal arbor growth and synaptogenesis in vivo

Ilya N. Kotov¹ and Douglas S. Campbell ^1,2

¹ Institute of Neuronal Cell Biology, Technical University of Munich, Munich, Germany

² Department of Neuronal Remodeling, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan

124

Functional analysis of the soluble protein Akhirin in the developing brain nerve stem cell niche

Mikiko Kudo ¹, Arif Istiaq^1,2, Kunimasa Ohta¹.

¹ Faculty of Arts & Science, Kyushu University

² Department of life science, Kumamoto University

125

Investigation of mesenchymal cell fate with FGF-8b supplementation in vitro

Takayoshi Otsuka ^{1, 2, 3}, Paulos Y. Mengsteab ^{1, 2, 3, 4}, and Cato T. Laurencin ^{1, 2, 3, 4, 5, 6}

¹ Connecticut Convergence Institute for Translation in Regenerative Engineering, University of Connecticut Health

² Raymond and Beverly Sackler Center for Biological, Physical and Engineering Sciences, University of Connecticut Health

³ Department of Orthopedic Surgery, University of Connecticut Health

⁴ Department of Biomedical Engineering, University of Connecticut

⁵ Department of Materials Science and Engineering, University of Connecticut

⁶ Department of Chemical and Biomolecular Engineering, University of Connecticut

129

Changes in the pattern of genes expression could provide the fore- and hind limbs digital patterns of the newt (Pleurodeles waltl)

Haruka Matsubara ¹ and Takashi Takeuchi ¹

¹ Division of Developmental Biology, Department of Biomedical Sciences, School of Life Science, Faculty of Medicine Tottori University

133

Polymorphism in the symmetries of gastric pouch arrangements in the sea anemone Diadumene lineata

Safiye E. Sarper^1,2, Tamami Hirai², Take Matsuyama², Shigeru Kuratani², and Koichi Fujimoto¹

¹Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560-0043, Japan

²RIKEN Center for Biosystems Dynamics Research (BDR)

134

Transcriptomic analyses of the fast-developing chordate, Oikopleura dioica, uncover re-organization of transcription factors functioning in early embryos and the animal-vegetal axis specification

Kai Wang¹, Ritsuko Suyama², Nakako Mizutani¹, Masaki Matsuo¹, Yu Peng¹, Masahide Seki³, Yutaka Suzuki³, Nicholas M. Luscombe², Christelle Dantec⁴, Patrick Lemaire⁴, Atsushi Toyoda⁵, Hiroki Nishida¹ and Takeshi A. Onuma*^{1, 6}

¹ Department of Biological Sciences, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560-0043, Japan

² Genomics and Regulatory Systems Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, Onna-son, Kunigami-gun, Okinawa 904-0495, Japan

³ Laboratory of Systems Genomics, Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan

⁴ Centre de Recherches de Biochimie Macromoleculaire (CRBM), UMR5237, CNRS-Universite de Montpellier, 1919 route de Mende, F-34090 Montpellier, France

⁵ Comparative Genomics Laboratory, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, Japan

⁶ Present address: Graduate School of Science and Engineering, Faculty of Science, Kagoshima University, 1–21–35 Korimoto, Kagoshima 890– 0065, Japan

136

Single-cell profiling of mouse oviduct epithelial cells

Masaki Arata ^{1, 2}, Fumiko Matsukawa Usami ^{1, 4}, Katsushi Yamaguchi ³, Shuji Shigenobu ^{3, 4}, Toshihiko Fujimori ^{1, 4}

¹ Division of Embryology, National Institute for Basic Biology

² Graduate School of Science, Nagoya University

³ NIBB Core Research Facilities, National Institute for Basic Biology

⁴ The Graduate University for Advanced Studies, SOKENDAI

137

Bioelectrical size regulation of bones and appendages in zebrafish

Toshihiro Aramaki¹ and Shigeru Kondo¹

¹ Graduate School of Frontier Biosciences, Osaka University

139

Gating mechanism of blastopore to regulate extracellular fluid dynamics

Soichiro Kato ^1,2, Hidehiko Inomata ^1,2

¹ Department of Biological Sciences, Graduate school of Science, Osaka university

² RIKEN BDR, Laboratory for Axial Pattern Dynamics

141

Cis-regulatory code for determining the action of Foxd as both an activator and a repressor in ascidian embryos

Shinichi Tokuhiro ¹ and Yutaka Satou ¹

¹ Department of Zoology, Graduate School of Science, Kyoto University

142

Two distinct motifs for Zic-r.a drive specific gene expression in two cell lineages

Izumi Oda-Ishii ¹, Deli-Yu ¹, and Yutaka Satou ¹

¹ Department of Zoology, Graduate School of Science, Kyoto University

143

Fbxo47 is essential for stability of the synaptonemal complex in male meiosis

Nobuhiro Tanno ¹, Kazumasa Takemoto ¹, Sayoko Fujimura ¹, Kimi Araki ^3,4, Kei-ichiro Ishiguro ¹

¹ Department of Chromosome Biology, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University

² Liaison Laboratory Research Promotion Center, IMEG, Kumamoto University

³ Center for Animal Resources and Development, Kumamoto University, Kumamoto

⁴ Center for metabolic Regulation of Healthy Aging, Kumamoto University, Kumamoto

144

NeuroGT: A brain atlas of neurogenic tagging CreER drivers for birthdate-based classification and manipulation of mouse neurons

Tatsumi Hirata¹, Yukako Tohsato², Hiroya Itoga³, Go Shioi³, Hiroshi Kiyonari³, Sanae Oka⁴, Toshihiko Fujimori⁴ and Shuich Onami³

¹ National Institute of Genetics

² Ritsumeikan University

³ RIKEN Center for Biosystems Dynamics Research

⁴ National Institute for Basic Biology

148

Meiosis-specific ZFP541 repressor complex promotes developmental progression of meiotic prophase towards completion during mouse spermatogenesis

Yuki Horisawa-Takada¹, Chisato Kodera¹, Kazumasa Takemoto¹, Akihiko Sakashita², Ryuki Shimada¹, Satoshi H. Namekawa³, Kimi Araki⁴ and Kei-ichiro Ishiguro¹

¹ Department of Chromosome Biology, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University

² Department of Molecular Biology, Keio University School of Medicine

³ Department of Microbiology and Molecular Genetics, University of California

⁴ Institute of Resource Development and Analysis, and Center for Metabolic Regulation of Healthy Aging, Kumamoto University

150

Repeated pulse illumination efficiently activates photoactivatable-Cre in vitro and in vivo

Kanae Kishi ^{1, 2}, Hiroshi Koyama ^{1, 3}, Sanae Oka ¹, Moritoshi Sato ⁴, and Toshihiko Fujimori ^{1, 2, 3}

¹ Division of Embryology, National Institute for Basic Biology

² Japan Science and Technology Agency, Core Research for Evolutional Science and Technology (JST-CREST)

³ The Graduate University for Advanced Studies, SOKENDAI

⁴ Graduate School of Arts and Sciences, The University of Tokyo

152

The evolution of lamprey vertebra

Hirofumi Kariyayama^1,2, Natalia Gogoleva^1,3, Keishi Harada¹, Hiroshi Wada¹

¹ Graduate School of Life and Environmental Science, University of Tsukuba

² Degree Programs in Medical Science, University of Tsukuba

³Ph.D. Program in Human Biology, University of Tsukuba

154

The dorsal arcopallium of chicks expresses the orthologues of mammalian fear-related 5-hydroxytryptamine receptor (5-HTR) genes

Shinji Yamaguchi ¹, Naoya Aoki ¹, Chiriro Mori ¹, Eiko Fujita ¹, Koichi J. Homma ¹, and Toshiyuki Fujita ¹

¹ Faculty of Pharmaceutical Sciences, Department of Life and Health Sciences, Teikyo University

155

Importance of paracrine function of Wnt3a in maintenance of the neuromesoderm progenitor population

Yudai Hatakeyama ^{1, 2, 3}, Yusuke Mii^{1, 2, 3, 4}, Shinji Takada^{1, 2, 3}

¹National Institute for Basic Biology, ²Exploratory Research Center on Life and Living Systems, NINS ³Graduate University for Advanced Studies (SOKENDAI), ⁴PREST, JST

157

A novel role of numb prevents the embryo from anti-neurogenic through the inhibition of Notch signaling downstream gene, tramtrack isoform 69

Elzava Y Mujizah ¹, Satoshi Kuwana ^1,3, Takuma Gushiken ¹, Kenjiroo Matsumoto ^1,4, Tomoko Yamakawa ¹, Martin Baron ², and Kenji Matsuno ¹

¹ Department of Biological Sciences, Osaka University

² Faculty of Life Sciences, University of Manchester

³ Department of Basic Science, University of Tokyo (current)

⁴ Department of Biochemistry and Molecular Biology, University of Georgia (current)

161

Analysis of genetic regulatory network of the “narigoma” enhancer responsible for left-right asymmetry of the anterior gut in Drosophila melanogaster

Florian Neugebauer ¹, Takuya Nomura ², Mikiko Inaki ³, Kenji Matsuno ⁴

¹ Department of Biological Sciences, Osaka University

² Department of Biological Sciences, Osaka University

³ Faculty of Science, Osaka University

⁴ Faculty of Science, Osaka University

162

Computational model of root hair morphogenesis

Hisako Takigawa-Imamura ¹, Tomoko Hirano ², and Masa H. Sato ²

¹ Department of Medical Sciences, Kyushu University

² Graduate School of Life and Environmental Sciences, Kyoto Prefectural University

163

Establishment of Knock-in Rats with CRISPR/Cas9 system in zygotes

Takaya Abe¹, Ken-ichi Inoue¹, and Hiroshi Kiyonari¹

¹Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Biosystems Dynamics Research

165

Investigating the molecular mechanisms regulating nutrient-dependent hyperarborization of somatosensory neurons in Drosophila larvae

Yasutetsu Kanaoka ¹, Henrik Skibbe ², Yusaku Hayashi ¹, Koun Onodera ¹, Ayumi Mure ¹, Yuuki Takahashi ¹, Tadao Usui ¹, Yukako Hattori ¹, and Tadashi Uemura ^1,3,4

¹ Graduate School of Biostudies, Kyoto University,

² Graduate School of Informatics, Kyoto University,

³ Research Center for Dynamic Living Systems, Kyoto University,

⁴ AMED-CREST

169

Functional analysis of Exocyst complex in mice spermatogenesis

Natsuki Mikami ¹, Kazuya Murata ², Masatsugu Ema ³, Satoru Takahashi ², Mizuno Seiya ², Sugiyama Fumihiro ²

¹ Ph.D Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba

² Laboratory Animal Resource Center, University of Tsukuba

³ Research Center for Animal Life Science, Shiga University of Medical Science

170

Extracellular phosphorylation drives neuronal circuit formation

Hidekiyo Harada ^1,2, Nahal Farhani¹, Xue-Fan Wang¹, Shuzo Sugita¹, Jason Charish^1,2,

Liliana Attisano ^3, Michael Moran⁴, Jean-Francois Cloutier⁵, Michael Reber^1,6,7, Rod Bremner⁸ and Philippe P. Monnier ^1,2,6*

¹ Krembil Research Institute, Vision Division, University Health Network

² Department of Physiology, Faculty of Medicine, University of Toronto

³ Department of Biochemistry, Donnelly Center, University of Toronto

⁴ Department of Molecular Genetics, University of Toronto

⁵ Montreal Neurological Institute

⁶ Department of Ophthalmology and Vision Sciences, Faculty of Medicine, University of Toronto

⁷ CNRS UPR3212, University of Strasbourg

⁸ Lunenfeld Tannenbaum Research Institute, Mount Sinai Hospital

171

Toward functional analysis in non-model organism, flounder

Michiharu Sekine ¹, Masaki Iwaizumi ¹, Minato Miyake ^1,2, Tohru Suzuki ¹, and Hayato Yokoi ¹

¹ Graduate School of Agricultural Science, Tohoku University

² Present address: Okinawa Institute of Science and Technology Graduate University

172

ECM remodeling drives epithelial unfolding of Drosophila imaginal discs during development

Emi Maekawa ¹, Katsuya Nozaki ¹, Raiki Yoshimura ¹, Megumi Nakayama ², Yuto Terada ¹, Tatsushi Igaki ², and Shizue Ohsawa ¹

¹ Genetics, Division of Biological Science, Graduate School of Science, Nagoya University

² Genetics, Graduate School of Biostudies, Kyoto University

175

Morphological complexity and its genetic background in vertebrate skeletal muscles

Rie Kusakabe ¹ and Shigeru Kuratani^{1, 2}

¹ Laboratory for Evolutionary Morphology, RIKEN BDR

² Evolutionary Morphology Laboratory, RIKEN CPR

176

Neurod1 expressing cells are a major source for the regenerating pancreatic β cell in zebrafish

Hiroki Matsuda

Department of Biomedical Sciences, College of Life Sciences, Ritsumeikan University

177

Admp regulates ventral curvature of ascidian tailbud embryo by controlling the cell polarity of ventral midline epidermal cells through myosin phosphorylation

Yuki Kogure ¹, Hiromochi Muraoka ¹, Wataru C. Koizumi ¹, Benoit Godard ², C. P. Heisenberg ², Kotaro Oka ^1,3,4 and Kohji Hotta ¹

¹ Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University, Kouhoku-ku, Yokohama 223-8522, Japan

² Institute of Science and Technology Austria, Klosterneuburg, Austria

³ Waseda Research Institute for Science and Engineering, Waseda University, 2-2 Wakamatsucho, Shinjuku, Tokyo 162-8480, Japan

⁴ Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung City 80708, Taiwan

180

Formal proof of the requirement of MESP1 and MESP2 in mesoderm specification and the transcriptional control via specific enhancers in mice.

Rieko Ajima^1,2, and Yumiko Saga^1,2

¹ Department of Gene Function and Phenomics, National Institute of Genetics, ROIS.

² Department of Genetics, SOKENDAI.

181

Synchronization dynamics of the zebrafish segmentation clock: effect of delayed coupling and period gradient

Koichiro Uriu ¹, Bo-Kai Liao ², Andrew C. Oates ³ and Luis G. Morelli ⁴

¹ Graduate School of Natural Science and Technology, Kanazawa University

² Department of Aquaculture, National Taiwan Ocean University

³ Institute of Bioengineering, École Polytechnique Fédérale de Lausanne

⁴ Instituto de Investigación en Biomedician de Buenos Aires

183

A novel creation of reporter mouse model for visualizing germ layer formation

Hayate Suzuki^1,2, Tra Thi Huong Dinh³, Yoko Daitoku³, Kanako Kato³, Yoko Tanimoto³, Kazuya Murata³, Seiya Mizuno³, and Fumihiro Sugiyama³

¹Doctor’s Program in Biomedical Sciences, University of Tsukuba

²Research Fellow of Japan Society for the Promotion of Science DC1

²Laboratory Animal Resource Center, Faculty of Medicine, University of Tsukuba

184

Oviductal glycine supports the embryonic development of fertilized eggs via glycine receptors on the cell membrane.

Hirofumi Nishizono ¹

¹ Medical Research Institute, Kanazawa Medical University

187

Global TCF/POP-1 polarity establishment by two Wnt receptors, Frizzled/MOM-5 and Ror1/CAM-1 in C. elegans mid-stage embryo

Takefumi Negishi ^1,2, Sohei Nakayama ¹, Misato Matsuo ¹ and Hitoshi Sawa ^1.2

¹ Multicellular Organization Laboratory, National Institute of Genetics

² Department of Genetics, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies)

190

Tiling mechanisms of the compound eye through geometrical tessellation

Takashi Hayashi ¹, Takeshi Tomomizu ², Takamichi Sushida ³, Masakazu Akiyama ⁴, Shin-Ichiro Ei ⁵, and Makoto Sato ¹

¹ Mathematical Neuroscience Unit, Institute for Frontier Science Initiative, Kanazawa University

² Graduate School of Frontier Science Initiative, Kanazawa University

³ Department of Computer Science and Technology, Salesian Polytechnic

⁴ Meiji Institute for Advanced Study of Mathematical Sciences, Meiji University

⁵ Department of Mathematics, Faculty of Science, Hokkaido University

194

Emergence of the median fin is owing to the primordia elongation rather than the carving out of the larval median fin fold.

Kazuhide Miyamoto ¹, Koichi Kawakami ^2,3, Gembu Abe ¹, and Koji Tamura ¹

¹ Department of Ecological Developmental Adaptability Life Sciences, Graduate School of Life Sciences, Tohoku University, Aobayama Aoba-ku, Sendai, 980-8578, Japan

² Laboratory of Molecular and Developmental Biology, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka, 411-8540, Japan.

³ Department of Genetics, The Graduate University for Advanced Studies, SOKENDAI, Mishima, Shizuoka, 411-8540, Japan

198

A single missense mutation of the RET gene in Hirschsprung disease induces intestinal aganglionosis via a dominant-negative mechanism

199

Interaction of cell migration with surrounding tissues during early cardiogenesis in zebrafish

Hitoshi Morita ¹

¹ Graduate School of Medical Sciences, University of Yamanashi

201

Airway basal stem cells reutilize the embryonic proliferation regulator, Tgfß-Id2 axis, for tissue regeneration

Hirofumi Kiyokawa¹, Akira Yamaoka¹, Chisa Matsuoka¹, Tomoko Tokuhara², Takaya Abe², Mitsuru Morimoto^1,*

¹ Laboratory for Lung Development and Regeneration, RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan

² Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan

202

Identification of protein motifs in MyosinIC and MyosinID responsible for left-right asymmetry of the hindgut in Drosophila

Asuka Yamaguchi ¹, Takeshi Sasamura ¹, Koji Ito ², Chinami Maeda ¹, Kenji Matsuno ¹

¹ Graduate School of Science, Osaka University

² Graduate School of Science, Chiba University

203

Live imaging of Ca²⁺ waves in the embryonic chick gut

Masafumi Inaba, Yuuki Shikaya and Yoshiko Takahashi

Department of Zoology, Graduate School of Science Kyoto University

204

Uncovering the role of PML bodies in gene expression by AP-CLaP

Misuzu Kurihara¹ and Yusuke Miyanari²

1 Faculty of Pharmaceutical Sciences, Hokkaido University

2 WPI Nano Life Science Institute, Kanazawa University

206

Identification of initial cue that leads to the left-right asymmetric morphogenesis of the embryonic midgut using a novel live-imaging analysis in Drosophila melanogaster

Takamasa Higashi ¹, Dongsun Shin¹, Mikiko Inaki ¹, Takuya Nomura ¹ ,Florian Neugebauer ¹, and Matsuno Kenji ¹

¹ Faculty of Science, Osaka University

210

Proper progress of mitosis ensured by greatwall kinase is important to prevent cell detachment from the axial tissue in zebrafish tailbud

Hideko Utsumi^1,2, Taijiro Yabe^1,2 and Shinji Takada^1,2

¹ Division of Molecular and Developmental Biology, National Institute for Basic Biology, National Institutes of Natural Sciences

² Developmental Signaling Research Group, Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences

213

The diversity of spot patterns in ladybirds can be explained by modulation of Turing patterns.

Ryo takeda

Oral Presenter ¹

¹ Department of Osaka University

214

Evolutionary History of GLIS1 and GLIS3 Illuminates Their Roles in Cell Reprograming and Ciliogenesis

Yuuri Yasuoka ¹, Masahito Matsumoto ^1,2, Ken Yagi ¹, Yasushi Okazaki ^1,2

¹ RIKEN Center for Integrative Medical Sciences

² Graduate School of Medicine, Juntendo University