XEN-EVIMEDI Kit
XEN-EVIMEDI Kit
Exosome Isolation
Product Description
Exosomes are nano-sized vesicles released into the extracellular environment e.g. body fluids including plasma, urine, saliva, and amniotic fluid. Initial findings merely identified them as cellular waste or by-products of cell activities, having little significance in the field. It is only recently that exosomes were shown to carry complex cargo of genetic materials, allowing intercellular communication between various target tissues. This exciting finding led to implication of exosomes as potential non-invasive diagnostic biomarkers and therapeutic nanocarriers. Despite numerous approaches to exosome isolation and growing number of related studies, their role as diagnostic biomarkers of disease remain incompletely understood. Nevertheless, as exosomes represent a novel platform of next-generation diagnostic tool, there’s an urgent need for optimal exosome isolation technique to take our step towards improving human health.
An intact isolation of exosome is a critical part of studying the biological function of exosomes. Until now, methods for EV isolation have been time-consuming, difficult and usually inconsistent due to ultracentrifugation step and non-specific reagents. XENO-EVIMEDI™ KIT is designed for optimized isolation of EVs from cell culture media.
Time saving : no cumbersome ultracentrifugation step
Cost-effective : more affordable than other costly kits
Broadly applicable : covers various media types
General Guidelines
The reaction size is based on using 500 uL of cell culture media sample.
To prevent contamination of exosomes, use exosome depleted fetal bovine serum (FBS) instead of normal FBS as they contain unwanted exosomes.
Related Product : XENO-EVI™ KIT, XENO-EVARI™ KIT, 10X XENO-EVARI Buffer
A and B. Exosomes were isolated from 1 ml of culture media using XENO-EVIDEMITM and kits from company A and company B. Nanoparticle tracking analysis (NTA) was performed to count the number of particles by using a TWIN ZetaView (PMX-230) system (Particle Metrix). Red circles indicate unfavorable fractions after exosome isolation.
C. TEM analyses of exosomes from two different types of neuronal cells. Size bar indicates 100 nm.