Product Description

The Xenohelix First-Strand Synthesis kit provides a high sensitivity and reproductivity, and bias-free cDNA synthesis for the two-step reverse transcription quantitative PCR (RT-qPCR). This kit includes three tubes, which contain all reagents required for successful reverse transcription. This kit offers outstanding results with high speed and convenience.

The Xenohelix Reverse transcriptase (RT) is a modified Moloney murine leukemia virus (MMLV) reverse transcriptase, optimized for reliable cDNA synthesis which covers a broad range of input RNA. This RTase enzyme has mutations within the RNase H domain, resulting in reduced RNase H activity and provides increased thermostability and higher yield of cDNA synthesis. With Xenohelix RT, synthesis of cDNA at temperature up to 50 ⁰C is possible, which results in improved outcome with RNA secondary structure. The enzyme is blended in advance with appropriate units of RNase inhibitor that prevent RNA from degradation. The optimized blend of random primers and oligo(dT) in the 4X Xeno RT Reaction Mix enables excellent performances with a wide range of targets. The Xeno cDNA synthesis Kit delivers superior results in both standard and real-time RT-qPCR.

Simple one tube protocol for cDNA synthesis under 26 minutes

• RNA concentration dependent results

• Reduced RNase H activity facilitates efficient cDNA synthesis up to 12kb

• Unique blending of oligo dT and random hexamer primers provides high specificity