XENOPURE™
XENOPURE™
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PLASMA/SERUM SMALL RNA
Product Description
XENOPURE™ Total RNA Purification Kit is a product that quickly and conveniently isolates high-quality total RNA (260/280 ratio>1.9) larger than 200 nt from various biological specimens, such as animal and plant cells, tissues, whole blood and bacteria.
In the process of sample lysis, XENOSEPA™-TR, which is a phenol-lysis buffer optimized for diverse samples, is used; and contaminants such as proteins and lipids are minimized by phase separation after adding chloroform. Moreover, extraction of high-purity RNA from plant samples containing large quantity of polyphenol, polysaccharide and lipid is possible.
In the process of RNA extraction, genomic DNA can be effectively removed by using spin-column which allows omission of DNase I enzyme treatment. The extracted RNA can be applied to various molecular biology and genetics experiments including northern blot, mRNA purification, in vitro translation, RT-PCR, real time PCR, and NGS.
High Yields
High purity sufficient for NGS, RT-PCR and related tests
No protease treatment required
Efficiently enrich small RNA
Product Performance
The XENOPURE Plasma Small RNA Purification Kit isolated more total RNAs and RNA spike-ins from 200 μl of human plasma than the competitor’s kit. For gel electrophoresis, 15% page-gel was used.
Total RNA in a human plasma sample was isolated using a XENOPURE Plasma Small RNA Purification Kit, and the presence of two target miRNAs was determined using a real-time PCR system.
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