XENO-EVI kit
XENO-EVI kit
Plasma/Serum
Product Description
Exosomes are nano-sized vesicles released into the extracellular environment e.g. body fluids including plasma, urine, saliva, and amniotic fluid. Initial findings merely identified them as cellular waste or by-products of cell activities, having little significance in the field. It is only recently that exosomes were shown to carry complex cargo of genetic materials, allowing intercellular communication between various target tissues. This exciting finding led to implication of exosomes as potential non-invasive diagnostic biomarkers and therapeutic nanocarriers. Despite numerous approaches to exosome isolation and growing number of related studies, their role as diagnostic biomarkers of disease remain incompletely understood. Nevertheless, as exosomes represent a novel platform of next-generation diagnostic tool, there’s an urgent need for optimal exosome isolation technique to take our step towards improving human health.
An intact isolation of exosome is a critical part of studying the biological function of exosomes. Until now, methods for EV isolation have been time-consuming, difficult and usually inconsistent due to ultracentrifugation step and non-specific reagents. XENO-EVI™ KIT is designed for optimized isolation of EVs from cell culture media.
Time saving : no cumbersome ultracentrifugation step
Cost-effective : more affordable than other costly kits
Higher purity : reduces carry-over of non-exosomal proteins
General Guidelines
The reaction size is based on using 100 uL of plasma sample.
Related Product : XENO-EVIMEDI™ KIT, XENO-EVARI™ KIT, 10X XENO-EVARI Buffer
To remove large vesicles and increase purity of exosomes, follow B. Exosome Isolation of our protocol.
Extracellular vesicles (EVs) isolation and characterization
EVs were isolated from 100 μl of plasma using XENO-EVI and characterized by using ZetaView® nanoparticle tracking analysis (NTA) instrument (Particle Metrix).
Western blot analysis of exosome and non-exosome markers in plasma and exosomes isolated from plasma.
Exosomes were isolated from 100 μl of plasma using XENO-EVI.
Analysis of exosome purity and the protein yield
Exosomes were isolated from 100 μl of plasma using XENO-EVI and kits from company A and company B. Nanoparticle tracking analysis (NTA) was performed to count the number of particles by using a NanoSight NS300 system (Malvern Instruments). Total protein concentration was measured at 280 nm.