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Bioreactor Characteristics
Bioreactor Characteristics
Media Consumption
Media Consumption
Media consumption approximated at different flow rates. Over three days, the faster flow rate showed to have the highest media consumption.
Residence Time with Different Flow Rates
Residence Time with Different Flow Rates
The residence time at different flow rates were measured. Take note that the reaction chamber volume of the PDMS chips are = 4,452 uL. Residence time exponentially decreases with flow rate.
Reynolds Number Vs. Flow Rate
Reynolds Number Vs. Flow Rate
The Reynolds number was approximated at different flow rates with our two different sized tubes of 0.3 mm and 0.8 mm inner diameter tubing. The fluid used was DMEM high glucose media. Through this figure, we can see that wide difference between 0.8 mm and 0.3 mm tubing. Near a flow rate of 250 uL/min the 0.8 mm tubing enters turbulent flow with a Re > 2000.
Cell Growth Characteristics: Batch Reactor Vs. Perfusion Bioreactor
Cell Growth Characteristics: Batch Reactor Vs. Perfusion Bioreactor
Cell Growth Full Model- Batch
Cell Growth Full Model- Batch
This is the cell growth model for the Batch reactor, showing the overall cell growth curve of a batch reactor until it reaches maximum confluency, shown in the horizontal line at the top. However, considering the experiments of interest are only around 3 days, we are only concerned with the initial growth of the curve, as we will compare with that of a perfusion bioreactor below.
3-Day Cell Growth Models
3-Day Cell Growth Models
3-Day Cell Growth: Batch Reactor
3-Day Cell Growth: Batch Reactor
This figure is a focused view of the above logistic growth batch reactor model for C2C12 cell growth. Each line represents a different initial cell seeding with cell number on the y-axis and time in the x-axis. This plot views the cell growth over 3 days, which was chosen for experimental relevance for future 3-day cell studies. It is noted that the cell growth in the batch reactor has a much slower rate as compared to the perfusion bioreactor and does not reach max confluency after this 3-day time period.
3-Day Cell Growth: Perfusion Bioreactor
3-Day Cell Growth: Perfusion Bioreactor
When taking samples of cells after three or more days, the perfusion bioreactor will exhibit a faster cell growth rate. When having an initial seeding of 100,000 cells, the perfusion bioreactor will nearly reach max confluency compared to the batch reactor. This shows the viability of the perfusion bioreactor to maintain and accelerate cell growth for a healthy cell environment.
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