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Batch reactors refer to culturing cells in a container with a constant volume with no addition or removal of media. This technique is widely used in both academic laboratory settings and industry.
Batch Growth Model
Batch Growth Model
Typical batch cultures follow similar growth patterns. The inoculums (seed organisms) uptake the dissolved nutrients within the medium and convert it into biomass. The figure on the left illustrates a mammalian batch growth model that has 5 phases (1) lag phase, (2) logarithmic/exponential phase, (3) deceleration phase, (4) stationary phase, and (5) death phase.
Importantly, throughout these phases, many organisms synthesize new enzymes. Since the environmental conditions change over time - nutrients are depleted, biomass increases, pH changes, enzyme concentration increases, etc - the intracellular machinery will adapt and regulate metabolic processes as a function of time. (note VCD = viable cell density in [cells/L]) [2].
Mathmatical Model
Mathmatical Model
Substate-limited growth model
Key Assumptions:
Constant Volume (dV/dt = 0)
No initial product (Po = 0)
Monod Kinetics
Negligable endogenous metabolism (kd =0)
Common Batch Reactor - Laboratory Setting
Common Batch Reactor - Laboratory Setting
This image is an image of a sequencing batch reactor (SBR). Typical SBRs are ysed in sludge process for the treatment of wastewater. The particular image on the left, is a laboratory scaled SBR [3].
Common Batch Reactor - Industry Setting
Common Batch Reactor - Industry Setting
This illustration is of the batch reactor geometry and the temperature control. In many industry settings, batch reactors are used like this due to the dynamics of non-isothermal operations [4].
References
References
[1] Feeder-Free Medium for Stem Cell Culture | Scientist Live. https://www.scientistlive.com/content/feeder-free-medium-stem-cell-culture. Accessed 14 May 2020.
[2] Bioprocess Engineering - 2nd Edition. https://www.elsevier.com/books/bioprocess-engineering/liu/978-0-444-63783-3. Accessed 14 May 2020.
[3] Laboratory Scale Experiments in Sequencing Batch Reactors. https://www.uv.es/joriber2/angles/info/labSBR.htm. Accessed 19 May 2020.
[4] Dimian, Alexandre C., et al. “Chapter 11 - Batch Processes.” Computer Aided Chemical Engineering, edited by Alexandre C. Dimian et al., vol. 35, Elsevier, 2014, pp. 449–88. ScienceDirect, doi:10.1016/B978-0-444-62700-1.00011-5.
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