Considerations

• Cancerous cells and other biologics can have adverse environmental effects if disposed of improperly

• Use of human cells for cancer studies may require patient consent to meet any privacy regulations

• No human or animal subjects used in our experiments, so IRB and IACUC approval are not applicable

• Other ethical considerations involving the acquisition of stem cells, however, our current experimentation does not involve these cell types

• Handling of biologics and chemicals

• Proper PPE to prevent contamination and exposure to hazardous materials

• Biological waste must be properly disposed of

• Utilize autoclave and other sterilization techniques to prevent contamination

• Safety concerns regarding mechanical components including high voltages and electrical shocks

• Important components such as the tubing, connectors, and pump come from reputable companies such as Qosina and Cole-Parmer, which are known to consistently produce standard-compliant products

• Tubing is autoclavable ensuring minimizing possibility of contamination

• 3D-printed customized mounts offer user ease of operation and safety control when extracting samples

• Microfluidic design is very resource efficient, minimizing both space and media consumption, while maintaining an n=3 platform to generate significant results

• Protocol produces consistent and precise results that provide for interfacing with other equipment

• Comparison between batch culture and bioreactor continuous culture can be achieved with standard techniques

Since our product can do four major things, we can convince industry to adapt the standard. These accomplishments include reducing the cost of manufacturing, parts, and consumables, increasing reliability of production, decreasing manual labor, and minimizing waste. By accomplishing these 4 things, we can show that our standard can bring value into this market space. It can be advantageous for others in the business to alter their protocols and adapt to this new standard.

• Appeals to buyers as it has high throughput with minimal waste

• Costs are greatly reduced by constructing our own PDMS slides